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Cel (NM_009885) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209300
Cel (Myc-DDK-tagged) - Mouse carboxyl ester lipase (Cel)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $558.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cel
Synonyms 1810036E18Rik; BAL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209300 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCGCCTGGAGGTTCTATTTCTTGGCCTCACCTGCTGCTTGGCAGCGGCTTGTGCTGCAAAGTTGG
GGGCTGTGTACACAGAAGGCGGTTTCGTGGAGGGCGTCAACAAGAAGCTCAGTCTCTTGGGTGGTGACTC
TGTTGACATCTTCAAGGGTATCCCCTTTGCCACTGCCAAGACCTTGGAGAATCCTCAGCGTCACCCTGGC
TGGCAAGGGACGCTGAAGGCTACAAACTTCAAGAAACGATGCCTACAGGCCACCATCACCCAGGACAACA
CCTATGGGCAAGAAGACTGCCTCTACCTCAATATCTGGGTGCCCCAGGGCAGGAAGCAGGTGTCCCACAA
CCTGCCTGTGATGGTCTGGATCTATGGAGGTGCCTTCCTCATGGGATCTGGCCAAGGGGCCAATTTTCTC
AAAAACTACCTGTATGACGGGGAGGAGATCGCCACTCGGGGCAACGTCATTGTGGTCACCTTCAACTACC
GTGTTGGACCCTTGGGTTTCCTTAGCACCGGGGATGCTAACCTTCCAGGTAACTTTGGTCTTCGAGATCA
GCACATGGCTATCGCCTGGGTGAAGAGGAACATTGCAGCCTTTGGGGGGGACCCTGATAACATCACCATC
TTTGGGGAATCTGCTGGAGCTGCCAGTGTCTCCCTGCAGACCCTCTCCCCATACAACAAGGGTCTCATCC
GGAGAGCCATCAGTCAGAGTGGTATGGCACTGAGCCCCTGGGCCATCCAGAAGAACCCACTTTTCTGGGC
CAAAACGATTGCTAAGAAGGTAGGATGCCCCACAGAGGATACTGGCAAGATGGCTGCGTGTCTGAAGATC
ACAGATCCCCGGGCCTTGACACTGGCCTACAAGTTGCCCGTGAAAAAGCAGGAGTACCCTGTTGTGCACT
ACCTGGCCTTCATCCCTGTCATCGATGGTGACTTCATTCCCGATGATCCCATCAACCTATACAACAACAC
TGCTGACATTGACTACATAGCGGGCATTAACAACATGGACGGCCACCTATTTGCTACCATTGATGTGCCC
GCTGTCGACAAGACCAAGCAGACTGTCACCGAGGAGGACTTCTACAGGCTAGTCAGTGGACACACTGTCG
CCAAGGGGCTGAAGGGGGCCCAAGCCACCTTTGACATCTACACTGAGTCCTGGGCCCAGGACCCGTCCCA
GGAGAACATGAAGAAGACAATGGTGGCCTTTGAGACTGACGTACTCTTCCTGATCCCCACGGAGATGGCG
CTGGCCCAGCACAAAGCCCATGCCAAGAGCGCCAAGACCTATTCTTACCTGTTTTCCCACCCTTCACGGA
TGCCTATCTACCCCAAATGGATGGGGGCAGACCATGCTGATGACCTCCAGTACGTCTTTGGAAAGCCTTT
TGCCACCCCACTGGGCTACCGGCCCCAAGATAGGGCTGTCTCCAAGGCCATGATCGCCTATTGGACCAAC
TTTGCCAGGAGTGGGGACCCCAACATGGGCAACTCACCTGTGCCCACACACTGGTACCCTTATACCTTGG
AGAATGGCAACTACCTGGACATCACTAAGACGATAACCAGCGCTTCCATGAAGGAGCACCTGAGAGAAAA
GTTCCTGAAGTTCTGGGCTGTGACATTCGAGGTGCTGCCCACAGTGACTGGTGACCAGGACACCCTCACT
CCCCCTGAGGATGACTCAGAGGTTGCCCCTGACCCACCTTCAGACGACTCCCAGGTTGTTCCTGTCCCAC
CTACAGATGACTCTGTGGAGGCTCAGATGCCTGCCACCATTGGCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209300 protein sequence
Red=Cloning site Green=Tags(s)

MGRLEVLFLGLTCCLAAACAAKLGAVYTEGGFVEGVNKKLSLLGGDSVDIFKGIPFATAKTLENPQRHPG
WQGTLKATNFKKRCLQATITQDNTYGQEDCLYLNIWVPQGRKQVSHNLPVMVWIYGGAFLMGSGQGANFL
KNYLYDGEEIATRGNVIVVTFNYRVGPLGFLSTGDANLPGNFGLRDQHMAIAWVKRNIAAFGGDPDNITI
FGESAGAASVSLQTLSPYNKGLIRRAISQSGMALSPWAIQKNPLFWAKTIAKKVGCPTEDTGKMAACLKI
TDPRALTLAYKLPVKKQEYPVVHYLAFIPVIDGDFIPDDPINLYNNTADIDYIAGINNMDGHLFATIDVP
AVDKTKQTVTEEDFYRLVSGHTVAKGLKGAQATFDIYTESWAQDPSQENMKKTMVAFETDVLFLIPTEMA
LAQHKAHAKSAKTYSYLFSHPSRMPIYPKWMGADHADDLQYVFGKPFATPLGYRPQDRAVSKAMIAYWTN
FARSGDPNMGNSPVPTHWYPYTLENGNYLDITKTITSASMKEHLREKFLKFWAVTFEVLPTVTGDQDTLT
PPEDDSEVAPDPPSDDSQVVPVPPTDDSVEAQMPATIGF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009885
ORF Size 1797 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009885.1, NM_009885.2, NP_034015.1
RefSeq Size 2046 bp
RefSeq ORF 1800 bp
Locus ID 12613
UniProt ID Q64285
Cytogenetics 2 19.38 cM
MW 65.9 kDa
Summary Catalyzes fat and vitamin absorption. Acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009885" in other vectors (4)
SKU Description Size Price
MC200295 Cel (untagged) - Mouse carboxyl ester lipase (Cel), (10ug) 10 ug
$558.00
MG209300 Cel (tGFP-tagged) - Mouse carboxyl ester lipase (Cel) 10 ug
$758.00
MR209300L3 Lenti ORF clone of Cel (Myc-DDK-tagged) - Mouse carboxyl ester lipase (Cel) 10 ug
$858.00
MR209300L4 Lenti ORF clone of Cel (mGFP-tagged) - Mouse carboxyl ester lipase (Cel) 10 ug
$858.00

Plasmid Map for MR209300

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209300
Circular map for MR209300

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