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Grk5 (NM_018869) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209199
Grk5 (Myc-DDK-tagged) - Mouse G protein-coupled receptor kinase 5 (Grk5)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $550.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Grk5
Synonyms Gprk5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209199 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCCGGGAAGGGGGTGGAGGAAAGCGCA
AAGGGAAAAGCAAGAAGTGGAAGGAAATCCTGAAGTTTCCTCACATCAGCCAGTGTGAAGACCTCCGAAG
GACCATAGACAGAGATTACTACAGTCTCTGTGACAAGCAACCAATTGGGAGACTGCTTTTTCGACAGTTC
TGTGAAACCAGGCCTGGGCTGGAGTGCTACATTCAGTTCCTGGACTTAGTGGCAGAATATGAAATTACTC
CAGATGAAAACCTTGGGGCGAAGGGGAAGGAAATAATGACCAAGTACCTCACTCCAAAGTCCCCAGTCTT
CATTGCCCAAGTTGGACAGGACCTGGTCTCCCAGACAGAGAAGAAGCTCCTGCAGAGCCCCTGCAAAGAA
CTCTTCTCTGCTTGTGCTCAGTCTGTCCATGACTACTTGACGGGAGACCCCTTCCACGAGTACCTGGATA
GCATGTATTTTGACCGTTTTCTGCAGTGGAAATGGTTAGAAAGACAACCAGTGACCAAAAACACTTTCCG
GCAGTACCGAGTGCTGGGCAAAGGGGGCTTTGGAGAGGTCTGTGCCTGCCAGGTTCGGGCCACTGGTAAA
ATGTATGCTTGTAAACGCTTAGAGAAGAAGAGGATCAAAAAGAGGAAAGGCGAATCCATGGCACTCAACG
AAAAGCAGATTCTTGAGAAGGTCAACAGCCAGTTTGTGGTCAACCTGGCCTATGCCTATGAAACCAAAGA
TGCACTATGCCTGGTTCTGACCATTATGAATGGTGGTGACCTGAAGTTTCACATCTACAATATGGGGAAT
CCTGGCTTTGAGGAAGAGCGAGCCTTATTTTATGCAGCTGAGATCCTCTGTGGCCTAGAAGACTTACACC
GTGAGAACACTGTCTATAGAGATCTAAAACCCGAAAACATCTTGCTGGATGATTATGGCCACATAAGGAT
CTCAGACCTCGGACTGGCCGTGAAGATCCCCGAGGGAGACCTTATCCGTGGCCGGGTAGGCACTGTTGGC
TACATGGCCCCAGAAGTTCTGAACAACCAGCGATATGGACTGAGCCCTGACTACTGGGGCCTGGGCTGCC
TCATCTATGAGATGATTGAAGGCCAGTCACCATTTCGAGGTCGCAAGGAGAAGGTTAAGCGGGAAGAGGT
GGATCGCCGGGTGCTGGAGACTGAGGAAGTATATTCCTCCAAGTTCTCTGAAGAGGCCAAGTCCATCTGC
AACATGCTGCTCACCAAAGACTCGAAGCAGAGGCTGGGCTGCCAGGAGGAGGGAGCCGCCGAGGTCAAGA
GGCACCCCTTCTTCAGGAACATGAACTTTAAGCGCCTGGAGGCTGGGATGTTGGACCCTCCCTTCGTTCC
AGATCCCCGGGCTGTATACTGCAAGGATGTGCTGGACATTGAGCAGTTCTCCACTGTGAAAGGTGTCAAC
CTGGACCATACGGACGATGATTTTTACTCAAAGTTCTCTACAGGCTCTGTGCCAATTCCATGGCAAAATG
AGATGATAGAAACAGAATGTTTCAAGGAGCTGAATGTGTTCGGACCTAACGGTACCCTCTCACCAGACCT
GAACAGAAGTCAGCCTCCAGAACCGCCAAAGAAAGGGCTGTTCCACAGACTCTTCAGGCGGCAGCATCAA
AGCAATTCCAAGAGTTCACCTACTCCTAAGACCAGTTGTAACCACCGAATAAATTCAAACCACATCAATT
CAAACTCCACTGGAAGCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209199 protein sequence
Red=Cloning site Green=Tags(s)

MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYYSLCDKQPIGRLLFRQF
CETRPGLECYIQFLDLVAEYEITPDENLGAKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEKKLLQSPCKE
LFSACAQSVHDYLTGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGK
MYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGN
PGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVG
YMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSIC
NMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVN
LDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQ
SNSKSSPTPKTSCNHRINSNHINSNSTGSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018869
ORF Size 1770 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018869.2
RefSeq Size 3182 bp
RefSeq ORF 1773 bp
Locus ID 14773
UniProt ID Q8VEB1
Cytogenetics 19 56.52 cM
MW 67.7 kDa
Summary Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).UniProtKB/Swiss-Prot Function
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"NM_018869" in other vectors (2)
SKU Description Size Price
MR209199L3 Lenti ORF clone of Grk5 (Myc-DDK-tagged) - Mouse G protein-coupled receptor kinase 5 (Grk5) 10 ug
$850.00
MR209199L4 Lenti ORF clone of Grk5 (mGFP-tagged) - Mouse G protein-coupled receptor kinase 5 (Grk5) 10 ug
$850.00

Plasmid Map for MR209199

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209199
Circular map for MR209199

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