Trim29 (NM_023655) Mouse Tagged ORF Clone
$874.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Trim29 |
| Synonyms | 1110047J21Rik; 2810431N19Rik; 4732461M22Rik; AI119726 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_023655 |
| ORF Size | 1761 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_023655.2, NP_076144.2 |
| RefSeq Size | 2830 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 72169 |
| UniProt ID | Q8R2Q0 |
| Cytogenetics | 9 A5.1 |
| MW | 65.8 kDa |
| Summary | Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of proinflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of TMEM173/STING in a similar way, leading to its degradation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_023655" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC219424 | Trim29 (untagged) - Mouse tripartite motif-containing 29 (Trim29), (10ug) | 10 ug |
$875.00
|
|
| MR209159L3 | Lenti ORF clone of Trim29 (Myc-DDK-tagged) - Mouse tripartite motif-containing 29 (Trim29) | 10 ug |
$1,174.00
|
|
| MR209159L4 | Lenti ORF clone of Trim29 (mGFP-tagged) - Mouse tripartite motif-containing 29 (Trim29) | 10 ug |
$1,174.00
|
|
Citations
TRIM29 negatively controls antiviral immune response through targeting STING for degradation
Li, Lin, Tong et al
Cell Discov (2018) 4, 13 DOI: 10.1038/s41421-018-0010-9
Li, Lin, Tong et al
Cell Discov (2018) 4, 13 DOI: 10.1038/s41421-018-0010-9
AI Summary: OriGene products, specifically the Myc-Flag-tagged TRIM29 plasmid and the pCDH-CMV-EF1-cop GFP expression vector, were used to express and study TRIM29 and its mutants, enabling investigation of TRIM29’s role as a negative regulator of antiviral immune responses in this study.
Snippet: … Plasmids Myc-Flag-tagged TRIM29 were from OriGene, different truncate TRIM29 mutants were cloned using the same vector, and mTRIM29 was cloned into pCDH-CMV-EF1-cop green fluorescent protein (GFP) (from OriGene) expression vector prepared for;... … Plasmids Myc-Flag-tagged TRIM29 were from OriGene, different truncate TRIM29 mutants were cloned using the same vector, and mTRIM29 was cloned into pCDH-CMV-EF1-cop green fluorescent protein (GFP) (from OriGene) expression vector prepared for
Showing 1-1 of 1 papers.
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