Mtpap (NM_026157) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR209138
Mtpap (Myc-DDK-tagged) - Mouse mitochondrial poly(A) polymerase (Mtpap), nuclear gene encoding mitochondrial protein
$289.00
$545.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Mtpap |
| Synonyms | 0610027A18Rik; AW551379; Papd1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_026157 |
| ORF Size | 1755 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_026157.3 |
| RefSeq Size | 2651 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 67440 |
| UniProt ID | Q9D0D3 |
| Cytogenetics | 18 A1 |
| MW | 65.2 kDa |
| Summary | Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_026157" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC202352 | Mtpap (untagged) - Mouse mitochondrial poly(A) polymerase (Mtpap), nuclear gene encoding mitochondrial protein, (10ug) | 10 ug |
$545.00
|
|
| MG209138 | Mtpap (tGFP-tagged) - Mouse PAP associated domain containing 1 (Papd1) | 10 ug |
$745.00
|
|
| MR209138L3 | Lenti ORF clone of Mtpap (Myc-DDK-tagged) - Mouse mitochondrial poly(A) polymerase (Mtpap), nuclear gene encoding mitochondrial protein | 10 ug |
$845.00
|
|
| MR209138L4 | Lenti ORF clone of Mtpap (mGFP-tagged) - Mouse mitochondrial poly(A) polymerase (Mtpap), nuclear gene encoding mitochondrial protein | 10 ug |
$845.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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