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Nop56 (NM_024193) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209091
Nop56 (Myc-DDK-tagged) - Mouse NOP56 ribonucleoprotein homolog (yeast) (Nop56)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $541.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nop56
Synonyms 2310044F10Rik; Nol5a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209091 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCTGCTGCACGTGCTGTTCGAGCATGCGGTCGGCTACGCGCTGTTGGCGCTGAAGGAAGTGGAGG
AGATCAGCCTGCTGCTGCCGCAGGTGGAGGAATGTGTGCTTAACCTTGGCAAATTCCACAATGTCGTTCG
TCTGGTGGCCTTCTGTCCCTTCTCTTCATCCCAGGTGGCCTTGGAAAATGCCAATGCTGTGTCTGAAGGT
GTTGTTCACGAGGACCTCCGCCTGCTCCTGGAGACATACCTGCCATCTAAAAAGAAGAAAGTACTTCTGG
GGGTTGGAGACCCCAAGATTGGTGCTGCAATACAGGAAGAGTTGGGGTACAACTGCCAGACTGGTGGTGT
GATAGCTGAGATCCTTCGAGGAGTTCGTCTGCATTTCCATAATCTGGTGAAGGGTCTGACAGATCTTTCT
GCTTGTAAGGCCCAGCTAGGGCTGGGACACAGCTACTCTCGTGCCAAAGTTAAATTTAATGTGAACCGAG
TGGACAACATGATTATTCAGTCCATAAGCCTCCTGGACCAACTGGATAAAGATATCAATACCTTCTCGAT
GCGTGTCAGGGAGTGGTATGGGTACCACTTTCCAGAGCTGGTGAAGATCGTCAATGACAACGCCACATAC
TGTCGCTTGGCTCAGTTCATTGGGAACCGCAGGGAGCTGAATGAAGAGAAGTTAGAGAAACTGGAAGAAA
TTACGATGGACGGGGCCAAGGCTAAGGCCATCTTGGATGCTTCTCGGTCCTCCATGGGCATGGACATATC
TGCTATCGACTTGATAAACATCGAGAGCTTCTCCAGTCGTGTGGTGTCTTTGTCAGAGTATCGCCAGAGC
CTACACACTTACCTTCGCTCCAAGATGAGCCAAGTAGCCCCCAGCCTGTCAGCCTTAATTGGGGAAGCGG
TAGGTGCACGCCTCATTGCTCATGCTGGCAGCCTCACCAACCTGGCCAAGTATCCAGCGTCCACAGTACA
GATCCTTGGGGCTGAAAAGGCCCTGTTCAGAGCCCTGAAGACAAGGGGTAACACACCAAAATATGGACTC
ATTTTTCATTCCACCTTCATTGGCCGAGCAGCTGCAAAGAACAAAGGCCGTATCTCCCGATACCTAGCAA
ACAAATGCAGTATTGCCTCAAGAATTGATTGCTTCTCTGAGGTCCCCACAAGTGTATTTGGGGAAAAACT
CCGAGAACAAGTTGAGGAACGGCTGTCTTTCTATGAGACTGGAGAGATCCCACGGAAGAACCTGGATGTC
ATGAAGGAAGCTGTGGTTCAGGCAGAGGAAGCGGCTGCTGAAATCGCCAGAAAGCTGGAAAAACAGGAGA
AGAAGCGCTTGAAGAAGGAAAAGAAGCGACTGGCTGCACTGGCCCTGGCCTCGTCAGAAAACAGCAGTAC
CCCAGAGGAGTGTGAGGAGGTAAATGAAAAATCTAAGAAGAAAAAGAAGTTAAAACCCCAGGAAAATGGA
ATGGAAGACCCACCTGTCTCTCTACCTAAATCCAAGAAAAAGAAAGCTCCCAAGGAGGAGTTGGCCAGTG
ACCTTGAAGAGATGGCCACTAGCAGTGCTAAGAGAAAGAAGTCCTCACCTAAGGAGGAAGTGGCCAGTGA
ACCAGAAGAGGCAGCAAGCCCCACCACCCCTAAGAAGAAAAGGAAATTTTCTGAGGAGCCTGAAGTTGCT
GCTAACTTCACAAAGAGCAGCACAAAGAAAAAGAAAAAGTCCCAGAAAGCACAGGAGGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209091 protein sequence
Red=Cloning site Green=Tags(s)

MVLLHVLFEHAVGYALLALKEVEEISLLLPQVEECVLNLGKFHNVVRLVAFCPFSSSQVALENANAVSEG
VVHEDLRLLLETYLPSKKKKVLLGVGDPKIGAAIQEELGYNCQTGGVIAEILRGVRLHFHNLVKGLTDLS
ACKAQLGLGHSYSRAKVKFNVNRVDNMIIQSISLLDQLDKDINTFSMRVREWYGYHFPELVKIVNDNATY
CRLAQFIGNRRELNEEKLEKLEEITMDGAKAKAILDASRSSMGMDISAIDLINIESFSSRVVSLSEYRQS
LHTYLRSKMSQVAPSLSALIGEAVGARLIAHAGSLTNLAKYPASTVQILGAEKALFRALKTRGNTPKYGL
IFHSTFIGRAAAKNKGRISRYLANKCSIASRIDCFSEVPTSVFGEKLREQVEERLSFYETGEIPRKNLDV
MKEAVVQAEEAAAEIARKLEKQEKKRLKKEKKRLAALALASSENSSTPEECEEVNEKSKKKKKLKPQENG
MEDPPVSLPKSKKKKAPKEELASDLEEMATSSAKRKKSSPKEEVASEPEEAASPTTPKKKRKFSEEPEVA
ANFTKSSTKKKKKSQKAQED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024193
ORF Size 1740 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024193.1
RefSeq Size 1904 bp
RefSeq ORF 1743 bp
Locus ID 67134
UniProt ID Q9D6Z1
Cytogenetics 2 F1
MW 64.4 kDa
Summary Involved in the early to middle stages of 60S ribosomal subunit biogenesis. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles. Required for the biogenesis of box C/D snoRNAs such U3, U8 and U14 snoRNAs (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024193" in other vectors (3)
SKU Description Size Price
MC205364 Nop56 (untagged) - Mouse NOP56 ribonucleoprotein homolog (yeast) (Nop56), (10ug) 10 ug
$541.00
MR209091L3 Lenti ORF clone of Nop56 (Myc-DDK-tagged) - Mouse NOP56 ribonucleoprotein homolog (yeast) (Nop56) 10 ug
$841.00
MR209091L4 Lenti ORF clone of Nop56 (mGFP-tagged) - Mouse NOP56 ribonucleoprotein homolog (yeast) (Nop56) 10 ug
$841.00

Plasmid Map for MR209091

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209091
Circular map for MR209091

file-alt Citations

The Diabetes Gene JAZF1 Is Essential for the Homeostatic Control of Ribosome Biogenesis and Function in Metabolic Stress
Kobiita, Godbersen, Araldi et al
Cell Rep (2020) 32 (1), 107846 DOI: 10.1016/j.celrep.2020.107846
Cited by 59
AI Summary: In this study, OriGene’s Myc-DDK-tagged expression plasmids for Nop56, Tsr3, Rps2, Rps11, Rps14, Rps15, Rps20, Rpl10a, Rpl12, Rpl13, and Rpl13a were used to investigate the role of specific ribosomal proteins and factors in elucidating how JAZF1 regulates ribosome biogenesis and function in pancreatic β-cells under metabolic stress.
Snippet: Jazf1 adenovirus: Ad: Jazf1-HA-tagged This paper N/A Gfp adenovirus: Ad:GFP This paper N/A Nop56 (Myc-DDK-tagged) Origene MR209091 Tsr3 (Myc-DDK-tagged) Origene MR204683 Rps2 (Myc-DDK-tagged) Origene MR204027 Rps11 (Myc-DDK-tagged) Origene MR216516 Rps14 (Myc-DDK-tagged) Origene RC203889 Rps15 (Myc-DDK-tagged) Origene MR200959 Rps20 (Myc-DDK-tagged) Origene MR200561 Rpl10a (Myc-DDK-tagged) Origene MR200782 Rpl12 (Myc-DDK-tagged) Origene MR201323 Rpl13 (Myc-DDK-tagged) Origene MR202263 Rpl13a (Myc-DDK-tagged) Origene MR202103
Showing 1-1 of 1 papers.
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