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Tpp1 (NM_009906) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208901
Tpp1 (Myc-DDK-tagged) - Mouse tripeptidyl peptidase I (Tpp1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $524.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tpp1
Synonyms Cl; Cln2; LPIC; TPP-1; TPP-I
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208901 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACTCCAAGCCCGCCTCCTAGGGCTCCTTGCTCTCGTCATCGCCGGCAAATGCACTTACAACCCTG
AGCCGGACCAGCGGTGGATGCTGCCTCCAGGCTGGGTGTCCCTGGGCCGCGTGGATCCCGAGGAAGAGCT
GAGTCTCACTTTTGCGCTGAAACAGCGGAACCTGGAAAGACTCTCGGAGCTGGTGCAGGCTGTGTCGGAT
CCTAGCTCTCCTCAATATGGAAAGTACCTAACCCTGGAGGATGTAGCTGAGCTGGTTCAACCATCACCCC
TGACCCTCCTCACTGTCCAAAAGTGGCTCTCAGCAGCTGGAGCCCGGAACTGCGATTCAGTGACCACCCA
GGACTTTCTGACTTGCTGGCTGAGTGTCCGACAGGCTGAGCTGCTGCTCCCAGGAGCTGAGTTTCATCGC
TATGTAGGGGGACCTACAAAGACCCATGTTATAAGGTCCCCACATCCCTACCAGCTTCCCCAGGCCTTGG
CCCCTCATGTGGATTTTGTGGGGGGGCTGCACCGTTTCCCCCCTTCATCTCCAAGACAACGTCCAGAACC
ACAACAGGTAGGAACTGTTAGCCTGCACTTGGGAGTGACTCCGTCTGTGCTCCGTCAGCGATACAACCTG
ACAGCCAAAGATGTGGGCTCAGGCACCACCAACAATAGCCAGGCCTGTGCCCAGTTCCTGGAACAGTACT
TCCATAACTCGGATCTGACTGAGTTCATGCGCCTATTCGGTGGCAGTTTTACACACCAGGCCTCAGTAGC
AAAAGTTGTTGGAAAGCAAGGGCGAGGCCGAGCTGGGATCGAGGCCAGTCTAGATGTGGAATACCTGATG
AGTGCTGGTGCCAATATCTCCACTTGGGTCTACAGTAGCCCTGGCCGCCATGAGGCACAGGAGCCCTTCT
TACAATGGCTCCTGCTTCTTAGCAATGAGTCATCTTTGCCACATGTACATACTGTGAGTTACGGAGACGA
TGAAGACTCCCTCAGCAGCATCTACATCCAGAGAGTCAACACTGAGTTCATGAAGGCTGCTGCTCGGGGT
CTCACCCTCCTTTTTGCCTCAGGTGACACTGGAGCTGGGTGTTGGTCTGTCTCCGGAAGACACAAGTTCC
GCCCTAGCTTCCCTGCTTCCAGCCCCTATGTTACTACAGTTGGAGGAACCTCCTTCAAGAATCCTTTCCT
CATCACAGATGAAGTAGTTGACTATATCAGTGGTGGAGGCTTCAGCAATGTTTTCCCACGGCCTCCCTAC
CAGGAGGAAGCAGTGGCCCAGTTCTTGAAATCCAGCTCTCATCTACCACCATCCAGTTACTTCAATGCTA
GTGGCCGTGCCTACCCAGATGTTGCCGCACTATCTGATGGCTACTGGGTGGTCAGCAACATGGTCCCCAT
TCCATGGGTATCTGGAACCTCGGCCTCTACTCCAGTGTTTGGGGGAATTTTATCCTTGATAAATGAGCAC
AGAATCCTCAATGGCCGCCCTCCTCTTGGCTTTCTCAACCCCAGGCTCTATCAGCAGCATGGGACAGGAC
TCTTTGATGTAACCCACGGCTGCCATGAGTCCTGTCTGAATGAAGAAGTGGAGGGTCAGGGTTTCTGCTC
TGGTCCTGGCTGGGATCCTGTGACAGGTTGGGGAACACCCAACTTCCCAGCCCTACTGAAGACCCTGCTC
AACCCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208901 protein sequence
Red=Cloning site Green=Tags(s)

MGLQARLLGLLALVIAGKCTYNPEPDQRWMLPPGWVSLGRVDPEEELSLTFALKQRNLERLSELVQAVSD
PSSPQYGKYLTLEDVAELVQPSPLTLLTVQKWLSAAGARNCDSVTTQDFLTCWLSVRQAELLLPGAEFHR
YVGGPTKTHVIRSPHPYQLPQALAPHVDFVGGLHRFPPSSPRQRPEPQQVGTVSLHLGVTPSVLRQRYNL
TAKDVGSGTTNNSQACAQFLEQYFHNSDLTEFMRLFGGSFTHQASVAKVVGKQGRGRAGIEASLDVEYLM
SAGANISTWVYSSPGRHEAQEPFLQWLLLLSNESSLPHVHTVSYGDDEDSLSSIYIQRVNTEFMKAAARG
LTLLFASGDTGAGCWSVSGRHKFRPSFPASSPYVTTVGGTSFKNPFLITDEVVDYISGGGFSNVFPRPPY
QEEAVAQFLKSSSHLPPSSYFNASGRAYPDVAALSDGYWVVSNMVPIPWVSGTSASTPVFGGILSLINEH
RILNGRPPLGFLNPRLYQQHGTGLFDVTHGCHESCLNEEVEGQGFCSGPGWDPVTGWGTPNFPALLKTLL
NP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009906
ORF Size 1686 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009906.6
RefSeq Size 3494 bp
RefSeq ORF 1689 bp
Locus ID 12751
UniProt ID O89023
Cytogenetics 7 55.97 cM
MW 61.3 kDa
Summary This gene encodes a lysosomal serine protease that cleaves N-terminal tripeptides from protein substrates. The encoded preproprotein undergoes autocatalytic processing to generate a mature enzyme. Mice lacking the encoded protein exhibit a progressive neurodegeneration and a greatly shortened lifespan. At the cellular level, mice lacking the encoded protein exhibit accumulation of autofluorescent lipopigments. Mutations in the human ortholog of this gene cause classical late-infantile neuronal ceroid lipofuscinosis. provided by RefSeq, Nov 2015
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Documents
Resources
"NM_009906" in other vectors (4)
SKU Description Size Price
MC204227 Tpp1 (untagged) - Mouse tripeptidyl peptidase I (Tpp1), (10ug) 10 ug
$524.00
MG208901 Tpp1 (tGFP-tagged) - Mouse tripeptidyl peptidase I (Tpp1) 10 ug
$724.00
MR208901L3 Lenti ORF clone of Tpp1 (Myc-DDK-tagged) - Mouse tripeptidyl peptidase I (Tpp1) 10 ug
$824.00
MR208901L4 Lenti ORF clone of Tpp1 (mGFP-tagged) - Mouse tripeptidyl peptidase I (Tpp1) 10 ug
$824.00

Plasmid Map for MR208901

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208901
Circular map for MR208901

file-alt Citations

Shelterin TPP1 Reduction Causes Telomere Attrition and Cellular Senescence via Sirt1 Deacetylase in Chronic Obstructive Pulmonary Disease
Ahmad, Sundar, Tormos et al
Am. J. Respir. Cell Mol. Biol. (2016)
AI Summary: OriGene's Myc-tagged TPP1 vectors were used to transfect cells, enabling the study of TPP1's role in telomere protection and cellular senescence in the context of COPD.
Snippet: used and treated with CSE with concentration of 0.5%, 0.25% and 0.2% for different experiments. Constructs and transfections Myc-tagged TPP1 vectors were obtained from Origene. HFL1 cells were transfected using various carriers as described in online data supplements ... Cite SaveSaving
Showing 1-1 of 1 papers.
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