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Ces1f (NM_144930) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208892
Ces1f (Myc-DDK-tagged) - Mouse carboxylesterase 1F (Ces1f)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $523.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ces1f
Synonyms AU018778; CesML1; TGH-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208892 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCTTAGCACTCTGTTCCTGGTGTCTCTAGCAACCTGTGTGATTTGCGGAAATCCCTCTTCACCAC
CTGTGGTAGACACTGCTCATGGTAAAGTCCTGGGGAAACACGTGAACGTAGAAGGATTTTCACAGCCTGT
GGCCGTCTTCCTGGGAATCCCCTTTGCCAAGCCCCCTCTTGGATCCCTGAGGTTTGCTCCACCACAGCCT
GCAGAGCCCTGGAGCTCAGTGAAGAATGCCACCACCTACCCACCTATGTGCTCCCAAGATGCAGCTAGAG
GACAGGCGGTCAATGACCTCATAACCAATAGAAAGGAGAAAATCCATCTTGAATTTTCTGAAGATTGCCT
GTACCTAAATATTTACACTCCTGCGGACTTTTCAAAGAACAGTAGGCTACCGGTGATGGTGTGGATCCAT
GGAGGTGGACTGAAGCTGGGTGGGGCATCAAGCTTTGATGGACGGGCTCTCTCTGCATACGAAAATGTGG
TGGTGGTGGCCATTCAATATCGCCTGAGTATCTGGGGATTCTTCAGCACAGGGGATGAACACAGTCGGGG
AAACTGGGGTCATTTGGACCAAGTGGCTGCTCTGCATTGGGTCCAGGACAACATTGCCAACTTTGGCGGG
GACCCAGGCTCTGTGACCATCTTTGGAGAGTCAGCAGGAGGTTACAGTGTCTCAATTCTTATATTGTCCC
CATTGTCCAAGAACCTCTTCCACAGGGCCATTTCTGAGAGTGGTGTGGCCTTCATTCCTGGAATGTTTAC
CAAAGATGTGAGGCCAATTACTGAGCAAATTGCTGTTACTGCTGGCTGTAAGACCACCACGTCTGCCGTC
ATTGTTCACTGCATGCGCCAGAAGACGGAGGAGGAGCTATTAGAGATCATGCATAAATTGAATCTGTATA
AACTGAGTTTACAAGGAGATACCAAAAATAGCGACCAGTTCGTGACAAGTGTGCTTGATGGAGTGGTGCT
ACCAAAGGACCCCAAAGAGATCCTGGCTGAGAAGAACTTCAACACTGTGCCTTACATTGTGGGAATCAAC
AAGCAAGAATGTGGCTGGCTTCTGCCAACAATGACGGGATTTCTACCAGCTGATGTAAAATTGGACAAGA
AGAAAGCCATTGCACTCCTGGAGCAATTTGCTTCCATGACTGGCATACCAGAGGATATTATTCCAGTTGC
TGTTGAGAAGTACACAAAAGGTAGTGATGACCCTGATCAGATCAGAGAGGGAGTTCTCGACGCAATGGGG
GATGTGGCATTTGGTGTTCCATCGGTGATTGTGTCCCGTGGCCACAGAGACACTGGAGCTCCCACCTACA
TGTATGAGTATCAATACTACCCAAGCTTCTCATCACCCCAAAGACCCAAGAATGTAGTAGGAGACCATGC
AGATGATGTCTACTCTGTCTTCGGTGCTCCAATTTTAAGAGAGGGTGCCTCCGAAGAGGAGATCAATCTC
AGCAAGATGGTGATGAAATTCTGGGCCAACTTTGCTCGGAATGGGAACCCTAATGGCAAAGGGCTGCCTC
ATTGGCCAAAGTATGATCAGAAAGAAGGATATCTTCATATTGGTGGCACCACCCAGCAAGCCCAGAGACT
GAAGGAAGAGGAAGTGACTTTCTGGACACAGTCCCTTGCCAAGAAACAACCCCAGCCATACCACAATGAG
CTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208892 protein sequence
Red=Cloning site Green=Tags(s)

MFLSTLFLVSLATCVICGNPSSPPVVDTAHGKVLGKHVNVEGFSQPVAVFLGIPFAKPPLGSLRFAPPQP
AEPWSSVKNATTYPPMCSQDAARGQAVNDLITNRKEKIHLEFSEDCLYLNIYTPADFSKNSRLPVMVWIH
GGGLKLGGASSFDGRALSAYENVVVVAIQYRLSIWGFFSTGDEHSRGNWGHLDQVAALHWVQDNIANFGG
DPGSVTIFGESAGGYSVSILILSPLSKNLFHRAISESGVAFIPGMFTKDVRPITEQIAVTAGCKTTTSAV
IVHCMRQKTEEELLEIMHKLNLYKLSLQGDTKNSDQFVTSVLDGVVLPKDPKEILAEKNFNTVPYIVGIN
KQECGWLLPTMTGFLPADVKLDKKKAIALLEQFASMTGIPEDIIPVAVEKYTKGSDDPDQIREGVLDAMG
DVAFGVPSVIVSRGHRDTGAPTYMYEYQYYPSFSSPQRPKNVVGDHADDVYSVFGAPILREGASEEEINL
SKMVMKFWANFARNGNPNGKGLPHWPKYDQKEGYLHIGGTTQQAQRLKEEEVTFWTQSLAKKQPQPYHNE
L

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144930
ORF Size 1683 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144930.2
RefSeq Size 1925 bp
RefSeq ORF 1686 bp
Locus ID 234564
UniProt ID Q91WU0
Cytogenetics 8 C5
MW 61.6 kDa
Summary Hydrolyzes p-nitrophenyl butyrate (PNPB), triacylglycerol and monoacylglycerol. Shows higher activity against PNPB, a short-chain fatty acid ester, than against triolein, a long-chain fatty acid ester. Shows no detectable activity against diacylglycerol, cholesterol ester or phospholipids. May play a role in adipocyte lipolysis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144930" in other vectors (3)
SKU Description Size Price
MG208892 Ces1f (tGFP-tagged) - Mouse expressed sequence AU018778 (AU018778) 10 ug
$723.00
MR208892L3 Lenti ORF clone of Ces1f (Myc-DDK-tagged) - Mouse carboxylesterase 1F (Ces1f) 10 ug
$823.00
MR208892L4 Lenti ORF clone of Ces1f (mGFP-tagged) - Mouse carboxylesterase 1F (Ces1f) 10 ug
$823.00

Plasmid Map for MR208892

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR208892
Circular map for MR208892

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