Npnt (NM_001029836) Mouse Tagged ORF Clone
$289.00
$523.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Npnt |
| Synonyms | 1110009H02Rik; AA682063; AI314031; Nctn; POEM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001029836 |
| ORF Size | 1683 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001029836.1, NM_001029836.2, NP_001025007.1 |
| RefSeq Size | 4619 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 114249 |
| UniProt ID | Q91V88 |
| Cytogenetics | 3 G3 |
| MW | 61.4 kDa |
| Summary | Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001029836" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC218928 | Npnt (untagged) - Mouse nephronectin (Npnt), transcript variant 2, (10ug) | 10 ug |
$575.00
|
|
| MG208888 | Npnt (tGFP-tagged) - Mouse nephronectin (Npnt), transcript variant 2 | 10 ug |
$723.00
|
|
| MR208888L3 | Lenti ORF clone of Npnt (Myc-DDK-tagged) - Mouse nephronectin (Npnt), transcript variant 2 | 10 ug |
$823.00
|
|
| MR208888L4 | Lenti ORF clone of Npnt (mGFP-tagged) - Mouse nephronectin (Npnt), transcript variant 2 | 10 ug |
$823.00
|
|
Citations
Phorbol-12-myristate 13-acetate inhibits Nephronectin gene expression via Protein kinase C alpha and c-Jun/c-Fos transcription factors
Kinoshita, Yamada, Sasa et al
Sci Rep (2021) 11 (1), 20360 DOI: 10.1038/s41598-021-00034-x
Kinoshita, Yamada, Sasa et al
Sci Rep (2021) 11 (1), 20360 DOI: 10.1038/s41598-021-00034-x
Cited by 6
AI Summary: OriGene’s pCMV6-Entry (Cat. No. PS100001) and Npnt-pCMV6-Entry (Cat. No. MR208888) expression vectors were used in this study to investigate the regulation of Nephronectin gene expression in osteoblasts.
Snippet: om R&D Systems, Inc. (Cat. No.355-BEC-010) and Gö6983 from Cayman Chemical, Inc. (Cat. No.13311). pCMV6-Entry (Cat. No. PS100001) and Npnt-pCMV6-Entry (Cat. No. MR208888) vectors were purchased from ORIGENE. Quantitative real-time PCR Total RNA was extracted from cells using TRIzol Reagent (Life Technologies, Cat. No. 15596018), then cDNA was synthesized using ReverTra Ace qPCR RT Master Mix
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
