Slc22a1 (NM_009202) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR208809
Slc22a1 (Myc-DDK-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1)
$289.00
$519.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Slc22a1 |
| Synonyms | Lx1; Oct1; Orct; Orct1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009202 |
| ORF Size | 1668 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009202.5 |
| RefSeq Size | 1994 bp |
| RefSeq ORF | 1671 bp |
| Locus ID | 20517 |
| UniProt ID | O08966 |
| Cytogenetics | 17 8.63 cM |
| MW | 61.5 kDa |
| Summary | Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009202" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC200831 | Slc22a1 (untagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1), (10ug) | 10 ug |
$519.00
|
|
| MG208809 | Slc22a1 (tGFP-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1) | 10 ug |
$719.00
|
|
| MR208809L3 | Lenti ORF clone of Slc22a1 (Myc-DDK-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1) | 10 ug |
$819.00
|
|
| MR208809L4 | Lenti ORF clone of Slc22a1 (mGFP-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1) | 10 ug |
$819.00
|
|
Citations
Fine Mapping and Functional Analysis Reveal a Role of SLC22A1 in Acylcarnitine Transport
Kim, Raffler, Lu et al
Am. J. Hum. Genet. (2017)
Kim, Raffler, Lu et al
Am. J. Hum. Genet. (2017)
AI Summary: OriGene's Mouse Slc22a1 cDNA was used in this study to generate adeno-associated virus (AAV) constructs for liver-specific expression, enabling functional analysis of SLC22A1's role in acylcarnitine transport.
Snippet: … Mouse Slc22a1 cDNA (Origene) was subcloned to an AAV vector plasmid provided by the Penn Vector core of the University of Pennsylvania. …Mouse Slc22a1 cDNA (Origene) was subcloned to an AAV vector plasmid provided by the Penn Vector core of the University of Pennsylvania. … Adeno-Associated Virus (AAV) Mouse Slc22a1 cDNA (Origene) was subcloned to an AAV vector plasmid provided by the Penn Vector core of the University of Pennsylvania. …
Sex-, Species-, and Tissue-Specific Metabolism of Empagliflozin in Male Mouse Kidney Forms an Unstable Hemiacetal Metabolite (M466/2) That Degrades to 4-Hydroxycrotonaldehyde, a Reactive and Cytotoxic Species
Taub, Ludwig-Schwellinger, Ishiguro et al
Chem. Res. Toxicol. (2015)
Taub, Ludwig-Schwellinger, Ishiguro et al
Chem. Res. Toxicol. (2015)
Cited by 34
AI Summary: OriGene contributed to this study by providing plasmid vectors (pCMV6-ENTRY) containing cDNA for various transporters—including oat1, oat3, oct1, oct2, oatp1a1, sglt1, and sglt2—which were used to investigate empagliflozin transport in transfected HEK293 cells.
Snippet: commercial suppliers. Plasmid vectors (pCMV6-ENTRY) containing cDNA for target transporters (oat1, oat3, oct1, oct2, oatp1a1, sglt1and sglt2) were obtained from Origene (Rockville, MD, USA). The plasmid vector, pSPORT1, containing either Oct1 or Oct2 were ... Cite SaveSaving
Showing 1-2 of 2 papers.
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