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Ttc26 (NM_153600) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208793
Ttc26 (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 26 (Ttc26)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $517.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ttc26
Synonyms 9330141E21Rik; 9430097H08Rik; hop; hpy
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208793 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCTTTCAAGGGCCAAACCCGCTGTGGGCGGGGAGTCACCGCACACTGACAAAAGAAAGAAGAAAG
GGAGGAAGATTCCAAAACTAGAGGATCTACTTTCGCAGAGAGATTTCACTGGCGCCATTACCCTATTGGA
GTTCAAGCGCCACGTTGGGGAGCAAGAAGACGACACTAACTTATGGATTGGATACTGTGCTTTTCACCTG
GGTGACTACAAGAGAGCTTTGGAGGAATATGAAAATGCAACGAAGGAGGAAAATTGCAATCCAGAAGTGT
GGGTGAACTTGGCCTGCACCTACTTTTTTCTTGGGATGTATAAACAAGCAGAAGCTGCTGGGTTTAAAGC
TCCAAAAAGCCGGCTCCAAAACCGCCTACTGTTCCATCTGGCTCACAAGTTTAATGATGAGAAGAAATTA
ATGAACTTTCATCAGAATCTTCAGGATATCAAGGAAGACCAGCTTAGTTTGGCCTCAATCCACTACATGC
GATCCCATTACCAAGAAGCTATTGATATATACAAGCGGATCCTGCTGGACAACAGGGAATACCTGGCCCT
CAATGTGTATGTGGCCCTCTGCTACTACAAACTGGATTACTATGATGTGTCTCAAGAAGTCCTGGCTGTT
TACCTTCAGCAGATTCCTGACAGTACCATCGCCCTCAACCTTAAGGCCTGCAATCACTTTCGCCTTTATA
ACGGCAAAGCAGCTGAGGCAGAGCTCAAAAGCTTGATGGACAATGCATCTTCACCCTTTGAATTTGCTAA
AGAACTCATCAGGCACAATCTGGTTGTTTTCCGAGGAGGCGAAGGGGCTTTGCAGGTTTTACCTCCCCTG
GTTGATGTCATTCCTGAAGCTAGGCTCAACCTAGTGATTTACTACCTTCGTCAAGATGATGTGCAGGAAG
CCTATAATTTAATCAAGGATTTGGAACCCACTACACCTCAGGAGTATATCCTCAAGGGAGTTGTCAATGC
AGCCCTTGGCCAGGAAATGGGTTCAAGGGATCATATGAAAATTGCCCAGCAGTTTTTCCAGTTGGTAGGA
GGATCAGCTAGTGAATGTGATACAATACCAGGAAGGCAGTGCATGGCTTCCTGTTTCTTCCTACTTAAAC
AATTTGATGATGTCTTGATTTACCTCAACTCTTTTAAGAGTTACTTCTATAATGATGACATCTTTAACTT
TAATTATGCCCAAGCCAAAGCTGCAACAGGCAATACCAGTGAGGGTGAAGAGGTTTTCCTCTTGATCCAA
AGTGAGAAACTGAAAAATGACTACATTTACCTTAGCTGGTTAGCTCGCTGCTATATTATGAATAAGAAAC
CAAGACTAGCTTGGGAGCTTTATCTCAAGATGGAAACCTCTGGCGAGTCCTTCAGCCTCTTACAGCTCAT
TGCCAATGACTGTTACAAGATGGGCCAGTTCTACTATTCAGCCAAAGCTTTTGATGTCTTAGAAAGACTG
GATCCCAACCCTGAGTACTGGGAAGGCAAGAGGGGTGCCTGTGTGGGCATCTTCCAGATGATCTTAGCTG
GGAGAGAACCCAAAGAGACCCTCCGAGAAGTGCTGCATTTACTGAGAAGCACAGGAAACACCCAAGTGGA
GTATATCATCAGGATCATGAAGAAGTGGGCCAAGGAGAATAGAGTGCCCATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208793 protein sequence
Red=Cloning site Green=Tags(s)

MMLSRAKPAVGGESPHTDKRKKKGRKIPKLEDLLSQRDFTGAITLLEFKRHVGEQEDDTNLWIGYCAFHL
GDYKRALEEYENATKEENCNPEVWVNLACTYFFLGMYKQAEAAGFKAPKSRLQNRLLFHLAHKFNDEKKL
MNFHQNLQDIKEDQLSLASIHYMRSHYQEAIDIYKRILLDNREYLALNVYVALCYYKLDYYDVSQEVLAV
YLQQIPDSTIALNLKACNHFRLYNGKAAEAELKSLMDNASSPFEFAKELIRHNLVVFRGGEGALQVLPPL
VDVIPEARLNLVIYYLRQDDVQEAYNLIKDLEPTTPQEYILKGVVNAALGQEMGSRDHMKIAQQFFQLVG
GSASECDTIPGRQCMASCFFLLKQFDDVLIYLNSFKSYFYNDDIFNFNYAQAKAATGNTSEGEEVFLLIQ
SEKLKNDYIYLSWLARCYIMNKKPRLAWELYLKMETSGESFSLLQLIANDCYKMGQFYYSAKAFDVLERL
DPNPEYWEGKRGACVGIFQMILAGREPKETLREVLHLLRSTGNTQVEYIIRIMKKWAKENRVPI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153600
ORF Size 1662 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153600.2, NP_705828.2
RefSeq Size 4162 bp
RefSeq ORF 1665 bp
Locus ID 264134
UniProt ID Q8BS45
Cytogenetics 6 B1
MW 64.2 kDa
Summary Component of the intraflagellar transport (IFT) complex B required for transport of proteins in the motile cilium. Required for transport of specific ciliary cargo proteins related to motility, while it is neither required for IFT complex B assembly or motion nor for cilium assembly. Required for efficient coupling between the accumulation of GLI2 and GLI3 at the ciliary tips and their dissociation from the negative regulator SUFU (PubMed:22718903, PubMed:25340710). Plays a key role in maintaining the integrity of the IFT complex B and the proper ciliary localization of the IFT complex B components. Not required for IFT complex A ciliary localization or function. Essential for maintaining proper microtubule organization within the ciliary axoneme (PubMed:28264835).UniProtKB/Swiss-Prot Function
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"NM_153600" in other vectors (4)
SKU Description Size Price
MC205317 Ttc26 (untagged) - Mouse tetratricopeptide repeat domain 26 (Ttc26), (10ug) 10 ug
$517.00
MG208793 Ttc26 (tGFP-tagged) - Mouse tetratricopeptide repeat domain 26 (Ttc26) 10 ug
$717.00
MR208793L3 Lenti ORF clone of Ttc26 (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 26 (Ttc26) 10 ug
$817.00
MR208793L4 Lenti ORF clone of Ttc26 (mGFP-tagged) - Mouse tetratricopeptide repeat domain 26 (Ttc26) 10 ug
$817.00

Plasmid Map for MR208793

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR208793
Circular map for MR208793

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