Foxc1 (NM_008592) Mouse Tagged ORF Clone
$772.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Foxc1 |
| Synonyms | ch; fkh-1; Fkh1; FREAC3; frkhda; Mf1; Mf4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008592 |
| ORF Size | 1659 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008592.2, NP_032618.2 |
| RefSeq Size | 3990 bp |
| RefSeq ORF | 1662 bp |
| Locus ID | 17300 |
| UniProt ID | Q61572 |
| Cytogenetics | 13 13.52 cM |
| MW | 57.4 kDa |
| Summary | DNA-binding transcriptional factor that plays a role in a broad range of cellular and developmental processes such as eye, bones, cardiovascular, kidney and skin development (PubMed:9635428, PubMed:9106663, PubMed:10479458, PubMed:10395790, PubMed:11562355, PubMed:18187037, PubMed:19668217, PubMed:22493429, PubMed:24590069, PubMed:25808752, PubMed:28223138). Acts either as a transcriptional activator or repressor (PubMed:28223138). Binds to the consensus binding site 5'-G/CA/TAAAT/CAAA/C-3' in promoter of target genes (PubMed:25808752). Upon DNA-binding, promotes DNA bending. Acts as a transcriptional coactivator (PubMed:25808752). Stimulates Indian hedgehog (Ihh)-induced target gene expression mediated by the transcription factor GLI2, and hence regulates endochondral ossification (PubMed:25808752). Acts also as a transcriptional coregulator by increasing DNA-binding capacity of GLI2 in breast cancer cells. Regulates FOXO1 through binding to a conserved element, 5'-GTAAACAAA-3' in its promoter region, implicating FOXC1 as an important regulator of cell viability and resistance to oxidative stress in the eye (By similarity). Cooperates with transcription factor FOXC2 in regulating expression of genes that maintain podocyte integrity (PubMed:28223138). Promotes cell growth inhibition by stopping the cell cycle in the G1 phase through TGFB1-mediated signals. Involved in epithelial-mesenchymal transition (EMT) induction by increasing cell proliferation, migration and invasion (By similarity). Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression (PubMed:18187037). Plays a role in the gene regulatory network essential for epidermal keratinocyte terminal differentiation (By similarity). Essential developmental transcriptional factor required for mesoderm-derived tissues formation, such as the somites, skin, bone and cartilage (PubMed:9106663, PubMed:10479458, PubMed:10395790, PubMed:10704385, PubMed:11562355, PubMed:15196959). Positively regulates CXCL12 and stem cell factor expression in bone marrow mesenchymal progenitor cells, and hence plays a role in the development and maintenance of mesenchymal niches for haematopoietic stem and progenitor cells (HSPC) (PubMed:24590069). Plays a role in corneal transparency by preventing both blood vessel and lymphatic vessel growth during embryonic development in a VEGF-dependent manner (PubMed:22171010). May function as a tumor suppressor (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008592" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC218787 | Foxc1 (untagged) - Mouse forkhead box C1 (Foxc1), (10ug) | 10 ug |
$567.00
|
|
| MG208772 | Foxc1 (tGFP-tagged) - Mouse forkhead box C1 (Foxc1) | 10 ug |
$972.00
|
|
| MR208772L1 | Lenti ORF clone of Foxc1 (Myc-DDK-tagged) - Mouse forkhead box C1 (Foxc1) | 10 ug |
$1,072.00
|
|
| MR208772L2 | Lenti ORF clone of Foxc1 (mGFP-tagged) - Mouse forkhead box C1 (Foxc1) | 10 ug |
$1,072.00
|
|
| MR208772L3 | Lenti ORF clone of Foxc1 (Myc-DDK-tagged) - Mouse forkhead box C1 (Foxc1) | 10 ug |
$1,072.00
|
|
| MR208772L4 | Lenti ORF clone of Foxc1 (mGFP-tagged) - Mouse forkhead box C1 (Foxc1) | 10 ug |
$1,072.00
|
|
Citations
Targeting EZH2 reactivates a breast cancer subtype-specific anti-metastatic transcriptional program
Hirukawa, Smith, Zuo et al
Nat Commun (2018) 9 (1), 2547 DOI: 10.1038/s41467-018-04864-8
Hirukawa, Smith, Zuo et al
Nat Commun (2018) 9 (1), 2547 DOI: 10.1038/s41467-018-04864-8
Cited by 84
AI Summary: In this study, OriGene products—including EZH2 shRNA (OriGene, #TL508431) and FOXC1 cDNA (OriGene, #RC215307)—were used to manipulate gene expression in breast cancer cells, enabling the authors to investigate the roles of EZH2 and FOXC1 in metastatic behavior and anti-metastatic transcriptional programs.
FOXC1-induced non-canonical WNT5A-MMP7 signaling regulates invasiveness in triple-negative breast cancer
Han, Zhou, Qu et al
Oncogene (2017)
Han, Zhou, Qu et al
Oncogene (2017)
Foxc1 Ablated Mice Are Anhidrotic and Recapitulate Features of Human Miliaria Sweat Retention Disorder
Cui, Ishii, Campbell et al
J. Invest. Dermatol. (2017) 137 (1), 38-45 DOI: 10.1016/j.jid.2016.08.012
Cui, Ishii, Campbell et al
J. Invest. Dermatol. (2017) 137 (1), 38-45 DOI: 10.1016/j.jid.2016.08.012
AI Summary: OriGene’s pCMV-Foxc1 plasmid was used in this study to overexpress Foxc1 in mouse keratinocytes, enabling investigation of Foxc1’s role in regulating sweat duct cell differentiation and modeling features of human miliaria.
Snippet: For Immunocytochemistry, mouse 308 keratinocytes were transfected with pCMV-Foxc1 plasmid (OriGene), fixed in 4% paraformaldehyde (MP Biomedicals), treated with 0.1% Triton X-100 (Sigma), and stained with Sprr2a/2b and Foxc1 antibodies
Showing 1-3 of 3 papers.
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