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Atg9a (BC079884) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208753
Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $513.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atg9a
Synonyms Atg9, Atg9l1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208753 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACAGTTTGACACTGAATACCAGCGCCTAGAGGCCTCCTATAGCGATTCGCCCCCCGGGGAAGAGG
ACCTGTTGGTGCATGTGGCCGAGGGGAGCAAATCACCTTGGCACCACATTGAAAACCTTGACCTCTTCTT
CTCTCGAGTTTATAATCTCCACCAGAAGAATGGCTTCACATGTATGCTCATTGGAGAGATGTTTGAACTC
ATGCAGTTCCTCTTTGTGGTTGCCTTCACCACCTTCCTGGTTAGCTGTGTGGACTATGACATCCTCTTTG
CCAACAAGATGGTGAACCACAGTCTCCATCCTACTGAGCCTGTCAAGGTCACTCTGCCAGATGCCTTTTT
GCCTGCCCAAGTCTGTAGTGCCAGGATTCAGGAAAATGGCTCTCTTATCACCATCCTGGTCATTGCTGGT
GTCTTCTGGATCCACCGGCTTATCAAGTTTATCTATAACATTTGCTGCTATTGGGAGATCCACTCCTTCT
ACCTACATGCTCTTCGGATCCCAATGTCTGCCCTTCCATACTGCACATGGCAGGAAGTTCAGGCCCGGAT
TGTGCAGACGCAGAAAGAGCATCAGATCTGCATCCACAAGCGTGAGCTGACAGAGTTGGACATCTACCAT
CGCATCCTACGTTTCCAGAACTACATGGTGGCCCTGGTGAACAAATCCCTCCTGCCTCTGCGCTTCCGTC
TGCCCGGCCTCGGAGAGGTTGTCTTCTTCACCCGTGGCCTCAAGTACAACTTTGAGCTCATCCTCTTCTG
GGGACCCGGCTCTCTGTTTCTCAATGAGTGGAGCCTCAAGGCCGAGTACAAACGTGGAGGGCAACGGCTA
GAGCTGGCCCAGCGTCTCAGCAACCGCATCTTGTGGATTGGCATCGCCAACTTCCTGCTGTGTCCCCTCA
TCCTCATCTGGCAGATCCTCTATGCCTTCTTCAGCTATGCCGAGGTGCTGAAGAGAGAGCCGGGGGCCCT
GGGAGCGCGTTGCTGGTCACTCTATGGCCGTTGTTACCTCCGCCACTTCAATGAGCTGGAGCATGAGCTG
CAGTCCCGCCTCAACCGAGGCTACAAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCGCTGCTGA
CTCTGCTGGCCAAGAATGGTGCCTTCTTCGCTGGCTCTATCCTGGCTGTGCTTATTGCCCTCACCATCTA
TGATGAAGATGTGTTAGCTGTGGAACACGTCCTCACCACGGTCACCCTCCTGGGAGTCACGGTGACTGTG
TGCAGGTCCTTCATCCCAGACCAGCACATGGTGTTCTGCCCCGAGCAGCTGCTCCGAGTGATTCTTGCAC
ACATCCACTACATGCCTGACCACTGGCAGGGTGTTCATCTTGGAGGAGTTGCTGAGTCCCATCGTCACAC
CCCTCATTCTCATCTTCTGCCTCCGCCCTCGGGCCCTGGAGATCATAGACTTCTTCCGCAACTTTACGGT
CGAGGTCGTGGGTGTGGGAGACACCTGCTCCTTTGCTCAGATGGACGTTCGCCAGCATGGCCATCCTCAG
TGGCTGTCTGGAGGGCAGACAGAGGCCTCAGTGTACCAGCAAGCCGAGGACGGGAAGACCGAGTTGTCGC
TCATGCACTTTGCCATCACCAATCCCGGCTGGCAGCCCCCTCG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208753 protein sequence
Red=Cloning site Green=Tags(s)

MAQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTCMLIGEMFEL
MQFLFVVAFTTFLVSCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQENGSLITILVIAG
VFWIHRLIKFIYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYH
RILRFQNYMVALVNKSLLPLRFRLPGLGEVVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRL
ELAQRLSNRILWIGIANFLLCPLILIWQILYAFFSYAEVLKREPGALGARCWSLYGRCYLRHFNELEHEL
QSRLNRGYKPASKYMNCFLSPLLTLLAKNGAFFAGSILAVLIALTIYDEDVLAVEHVLTTVTLLGVTVTV
CRSFIPDQHMVFCPEQLLRVILAHIHYMPDHWQGVHLGGVAESHRHTPHSHLLPPPSGPGDHRLLPQLYG
RGRGCGRHLLLCSDGRSPAWPSSVAVWRADRGLSVPASRGREDRVVAHALCHHQSRLAAPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC079884
ORF Size 1653 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC079884, AAH79884
RefSeq Size 3792 bp
RefSeq ORF 1655 bp
Locus ID 245860
Cytogenetics 1 C4
MW 63.1 kDa
Summary Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H (By similarity). Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP)-induced ATG8 family proteins lipidation, a key autophagy step.UniProtKB/Swiss-Prot Function
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"BC079884" in other vectors (6)
SKU Description Size Price
MC218655 Atg9a (untagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050), (10ug) 10 ug
$565.00
MG208753 Atg9a (tGFP-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) 10 ug
$713.00
MR208753L1 Lenti ORF clone of Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) 10 ug
$813.00
MR208753L2 Lenti ORF clone of Atg9a (mGFP-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) 10 ug
$813.00
MR208753L3 Lenti ORF clone of Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) 10 ug
$813.00
MR208753L4 Lenti ORF clone of Atg9a (mGFP-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) 10 ug
$813.00

Plasmid Map for MR208753

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file-download TXT (FASTA format)

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Cloning scheme for MR208753
Circular map for MR208753

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