Pip5k1a (NM_008847) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR208688
Pip5k1a (Myc-DDK-tagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha (Pip5k1a)
$289.00
$509.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Pip5k1a |
| Synonyms | PI4P5K-Ia; PIP5K1-alpha; PIP5KIalpha; Pipk5a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008847 |
| ORF Size | 1638 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008847.3 |
| RefSeq Size | 3673 bp |
| RefSeq ORF | 1641 bp |
| Locus ID | 18720 |
| UniProt ID | P70182 |
| Cytogenetics | 3 F2.1 |
| MW | 60.5 kDa |
| Summary | Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs (PubMed:10679324, PubMed:18772378, PubMed:19153220, PubMed:20622009, PubMed:8798574). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes (PubMed:27739494).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008847" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC201475 | Pip5k1a (untagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha (Pip5k1a), (10ug) | 10 ug |
$509.00
|
|
| MG208688 | Pip5k1a (tGFP-tagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha (Pip5k1a) | 10 ug |
$709.00
|
|
| MR208688L3 | Lenti ORF clone of Pip5k1a (Myc-DDK-tagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha (Pip5k1a) | 10 ug |
$809.00
|
|
| MR208688L4 | Lenti ORF clone of Pip5k1a (mGFP-tagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha (Pip5k1a) | 10 ug |
$809.00
|
|
Citations
Pip5k1γ regulates axon formation by limiting Rap1 activity
Meo, Kundu, Ravindran et al
Life Science Alliance (2024) 7 (5), e202302383-e202302383 DOI: 10.26508/lsa.202302383
Meo, Kundu, Ravindran et al
Life Science Alliance (2024) 7 (5), e202302383-e202302383 DOI: 10.26508/lsa.202302383
Cited by 2
AI Summary: OriGene products pMyc-DDK-Pip5k1a (#MR208688), pMyc-DDK-Pip5k1b (#MR20779), and pMyc-DDK-Pip5k1c (#MR209646) were used to generate RNAi-resistant Pip5k1α, Pip5k1β, and Pip5k1γ constructs, enabling the study of Pip5k1γ’s role in regulating axon formation by limiting Rap1 activity.
Snippet: …Pip5k1α, Pip5k1β, and Pip5k1γ RNAi-resistant constructs were generated from pMyc-DDK-Pip5k1a (#MR208688;OriGene), pMyc-DDK-Pip5k1b (#MR20779;OriGene), and pMyc-DDK-Pip5k1c (#MR209646;OriGene), respectively. To express C3G (Rapgef1), the complete mouse C3G coding sequence (accession number BC129881) was cloned in the pEGFP-C2 expression vector
Showing 1-1 of 1 papers.
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