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Ehd4 (NM_133838) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208637
Ehd4 (Myc-DDK-tagged) - Mouse EH-domain containing 4 (Ehd4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $505.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ehd4
Synonyms 2210022F10Rik; AI197390; AI846352; AV006278; Past2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208637 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCAGCTGGATGGGGCGGCAGGCTGGCGGGCGCGAGCGCTCGGGCGGCATGGACGCGGTGCAGACGG
TGACGGGCGGCCTGCGCTCTCTGTACCAGCGCAAGGTGCTGCCGCTGGAGGAGGCGTACCGCTTCCACGA
GTTCCACTCGCCAGCGCTGGAGGACGCCGACTTCGAAAACAAGCCCATGATCCTGCTGGTGGGCCAGTAC
AGCACGGGCAAGACCACGTTCATCAGATACTTACTGGAGCAAGATTTCCCAGGCATGAGAATTGGTCCAG
AGCCAACCACAGATTCCTTCATTGCTGTGATGTATGGGGAGACTGAGGGAAGCACCCCTGGGAATGCTTT
AGTTGTGGATCCCAAAAAGCCATTCCGAAAGCTTAGTCGCTTTGGAAATGCTTTCCTGAACAGATTCATG
TGCTCACAGCTGCCCAACCAAGTTCTGAAGAGCATCAGCATCATCGACAGTCCTGGCATTCTGTCTGGGG
AGAAGCAGCGCATAAGCCGAGGGTATGACTTCTGCCAGGTCTTACAGTGGTTTGCCGAGAGGGTTGACAG
GATCATCCTGCTCTTTGACGCTCACAAATTGGACATCTCAGATGAGTTCTCAGAGGCGATCAAGGCCTTC
CGGGGCCAGGATGACAAAATCCGAGTGGTACTGAACAAGGCTGACCAAGTGGATACACAGCAGCTGATGC
GAGTCTATGGGGCCCTCATGTGGTCTCTGGGCAAGGTCATCAACACACCTGAAGTACTTCGAGTCTACAT
TGGTTCATTTTGGGCACAGCCCCTGCAAAACACCGACAACCGCCGGCTCTTTGAGGCTGAAGCACAGGAC
CTCTTCCGAGACATCCAGAGCCTGCCCCAGAAGGCTGCAGTGCGCAAACTCAATGACCTCATCAAGCGTG
CAAGGTTGGCCAAAGTCCACGCCTACATCATCAGCTACCTGAAGAAGGAAATGCCAAATATGTTTGGAAA
AGAAAATAAGAAGCGAGAACTTATCTACAGGCTCCCGGAAATCTATGTTCAGCTGCAGCGAGAATACCAG
ATTTCTGCAGGGGACTTCCCTGAGGTCAAGGCCATGCAGGAGCAGCTGGAGAACTACGACTTCACCAAGT
TCCACTCACTGAAGCCCAAGCTGATCGAGGCTGTGGACAACATGCTGACCAACAAGATCTCATCCCTGAT
GGGCCTCATCAGCCAGGAGGAGATGAACATGCCCACGCAGATGGTGCAGGGCGGCGCCTTTGATGGCACC
ACAGAGGGGCCCTTCAACCAGGGCTATGGGGAGGGGGCCAAGGAGGGGGCCGATGAGGAAGAGTGGGTCG
TGGCTAAAGACAAGCCGGTGTATGACGAACTCTTTTACACGCTGTCACCCATCAATGGCAAGATATCGGG
TGTCAACGCCAAGAAGGAGATGGTGACCTCCAAGCTGCCCAACAGTGTCCTGGGCAAGATCTGGAAGCTG
GCCGACTGCGACTGCGACGGCATGCTAGACGAGGAGGAGTTCGCGCTGGCCAAGCACCTCATCAAGATCA
AGCTGGACGGCTACGAGCTGCCCAACAGCCTGCCCCCACACCTGGTGCCCCCCTCCCACAGGAAGTCCCT
GCCAAAGGCTGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208637 protein sequence
Red=Cloning site Green=Tags(s)

MFSWMGRQAGGRERSGGMDAVQTVTGGLRSLYQRKVLPLEEAYRFHEFHSPALEDADFENKPMILLVGQY
STGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMYGETEGSTPGNALVVDPKKPFRKLSRFGNAFLNRFM
CSQLPNQVLKSISIIDSPGILSGEKQRISRGYDFCQVLQWFAERVDRIILLFDAHKLDISDEFSEAIKAF
RGQDDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVINTPEVLRVYIGSFWAQPLQNTDNRRLFEAEAQD
LFRDIQSLPQKAAVRKLNDLIKRARLAKVHAYIISYLKKEMPNMFGKENKKRELIYRLPEIYVQLQREYQ
ISAGDFPEVKAMQEQLENYDFTKFHSLKPKLIEAVDNMLTNKISSLMGLISQEEMNMPTQMVQGGAFDGT
TEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKL
ADCDCDGMLDEEEFALAKHLIKIKLDGYELPNSLPPHLVPPSHRKSLPKAD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133838
ORF Size 1623 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133838.3
RefSeq Size 3387 bp
RefSeq ORF 1626 bp
Locus ID 98878
UniProt ID Q9EQP2
Cytogenetics 2 E5
MW 61.5 kDa
Summary ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133838" in other vectors (4)
SKU Description Size Price
MC203670 Ehd4 (untagged) - Mouse EH-domain containing 4 (Ehd4), (10ug) 10 ug
$505.00
MG208637 Ehd4 (tGFP-tagged) - Mouse EH-domain containing 4 (Ehd4) 10 ug
$705.00
MR208637L3 Lenti ORF clone of Ehd4 (Myc-DDK-tagged) - Mouse EH-domain containing 4 (Ehd4) 10 ug
$805.00
MR208637L4 Lenti ORF clone of Ehd4 (mGFP-tagged) - Mouse EH-domain containing 4 (Ehd4) 10 ug
$805.00

Plasmid Map for MR208637

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208637
Circular map for MR208637

file-alt Citations

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