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Galnt2 (BC053063) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208588
Galnt2 (Myc-DDK-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (cDNA clone MGC:62366
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $499.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Galnt2
Synonyms AI480629
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208588 representing BC053063
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTCCACAACCCACAGGGTGACTGGAATGACATCGACTCCATTAAGAAGAAAGACCTTCACCACA
GCAGAGGAGATGAGAAAGCACAGGGTGTGGAGACCCTCCCTCCAGGCAAAGTCCGGTGGCCAGACTTTAA
CCAGGAGGCATACGTTGGCGGTACAATGGTTCGCTCTGGGCAGGACCCGTACGCACGCAACAAGTTCAAC
CAGGTGGAGAGTGACAAGCTGCACATGGACAGAGGCATCCCCGACACCCGGCATGACCAGTGTCAGCGCA
AGCAGTGGCGAGTGGACCTGCCAGCCACCAGCGTGGTGATCACATTCCACAATGAAGCCAGGTCGGCCCT
GCTGCGGACGGTGGTCAGTGTCCTCAAGAGGAGTCCACCTCACCTCATCAAGGAGATAATCCTGGTGGAT
GACTACAGCAACGACCCTGAGGACGGGGCCCTCTTGGGGAAAATCGAGAAGGTGCGGGTCCTCAGAAATG
ACCGGAGAGAAGGTCTAATGCGCTCTCGGGTCCGGGGTGCCGACGCCGCCCAGGCCAAGGTCCTGACCTT
CCTAGACAGTCACTGCGAGTGTAACGAGCGATGGCTGGAGCCACTCTTGGAGCGGGTTGCTGAGGACAGG
ACCCGAGTTGTATCCCCCATTATCGATGTCATTAACATGGATAATTTCCAGTATGTGGGCGCCTCTGCTG
ACCTCAAGGGTGGTTTTGACTGGAACTTGGTGTTCAAGTGGGATTACATGACACCAGAGCAGAGGCGATC
CCGGCAGGGGAACCCAGTTGCCCCTATAAAAACTCCGATGATCGCTGGCGGCCTCTTCGTGATGGACAAG
CTCTATTTTGAAGAGCTGGGGAAGTACGACATGATGATGGACGTGTGGGGAGGAGAGAACTTGGAGATCT
CATTCCGAGTGTGGCAGTGTGGTGGCAGCCTGGAGATCATCCCGTGCAGCCGCGTGGGCCACGTGTTCCG
GAAGCAGCATCCCTACACGTTCCCCGGTGGCAGCGGCACTGTCTTTGCCCGGAACACACGCCGGGCAGCT
GAAGTCTGGATGGATGAGTACAAACATTTCTACTACGCAGCGGTGCCTTCTGCACGAAACGTGCCCTATG
GGAATATTCAGAGCAGGCTGGAGCTCAGGAAAAAGCTGGGCTGCAAGCCCTTCAAGTGGTACCTGGACAA
CGTCTACCCGGAGCTGAGGGTTCCAGACCACCAGGACATAGCTTTCGGGGCCTTACAGCAGGGAACCAAC
TGCCTGGACACCTTGGGACACTTCGCGGATGGAGTTGTTGGAATTTATGAGTGTCACAACGCTGGAGGAA
ACCAGGAATGGGCCTTGACAAAGGAGAAGTCGGTGAAGCACATGGACCTGTGCCTTACTGTGGTGGACCG
TTCGCCTGGGTCACTCATCAGGCTGCAGGGCTGCCGGGAGAACGACAGCAGACAGAAGTGGGAGCAGATC
GAAGGCAACTCCAAACTGCGACACGTGGGCAGCAACCTGTGCCTGGATAGCCGCACTGCCAAGAGCGGAG
GCCTGAGTGTGGAGGTGTGTGGCCCGGCCCTGTCACAACAGTGGAAGTTCTCACTCAACCTGCAGCAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208588 representing BC053063
Red=Cloning site Green=Tags(s)

MALHNPQGDWNDIDSIKKKDLHHSRGDEKAQGVETLPPGKVRWPDFNQEAYVGGTMVRSGQDPYARNKFN
QVESDKLHMDRGIPDTRHDQCQRKQWRVDLPATSVVITFHNEARSALLRTVVSVLKRSPPHLIKEIILVD
DYSNDPEDGALLGKIEKVRVLRNDRREGLMRSRVRGADAAQAKVLTFLDSHCECNERWLEPLLERVAEDR
TRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDK
LYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAA
EVWMDEYKHFYYAAVPSARNVPYGNIQSRLELRKKLGCKPFKWYLDNVYPELRVPDHQDIAFGALQQGTN
CLDTLGHFADGVVGIYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRSPGSLIRLQGCRENDSRQKWEQI
EGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFSLNLQQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC053063
ORF Size 1608 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC053063.1
RefSeq Size 3975 bp
RefSeq ORF 1610 bp
Locus ID 108148
Cytogenetics 8 E2
MW 145.8 kDa
Summary Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region (By similarity).UniProtKB/Swiss-Prot Function
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"BC053063" in other vectors (4)
SKU Description Size Price
MC218529 Galnt2 (untagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (cDNA clone MGC:62366, (10ug) 10 ug
$550.00
MG208588 Galnt2 (tGFP-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (cDNA clone MGC:62366 10 ug
$699.00
MR208588L3 Lenti ORF clone of Galnt2 (Myc-DDK-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (cDNA clone MGC:62366 10 ug
$799.00
MR208588L4 Lenti ORF clone of Galnt2 (mGFP-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (cDNA clone MGC:62366 10 ug
$799.00

Plasmid Map for MR208588

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208588
Circular map for MR208588

file-alt Citations

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