Ehd3 (NM_020578) Mouse Tagged ORF Clone

SKU MR208579

Ehd3 (Myc-DDK-tagged) - Mouse EH-domain containing 3 (Ehd3)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ehd3
• Synonyms: Ehd2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208579 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTCAGTTGGCTGGGTAACGATGATCGCCGCAAGAAGGACCCTGAGGTCTTCCAGACGGTGAGCGATG; GACTCAAAAAACTCTACAAGACCAAGCTGCTGCCTCTGGAAGAGTATTACCGCTTCCACGAGTTCCACTC; GCCCGCCCTGGAAGATGCTGATTTCGACAACAAGCCCATGGTCCTGTTGGTGGGCCAGTACTCTACCGGC; AAGACCACCTTCATCAGGTACCTGCTGGAACAGGATTTTCCAGGCATGAGGATTGGGCCTGAGCCGACCA; CTGATTCCTTCATAGCAGTGATGCAGGGAGATGTGGAGGGGATCATTCCCGGGAATGCCTTGGTGGTGGA; TCCAAAGAAACCCTTCAGAAAGCTCAACGCCTTTGGCAACGCCTTCCTGAACAGGTTTGTGTGTGCCCAG; CTGCCCAACGCCGTGCTTGAAAGTATCAGTGTGATCGACACACCGGGGATCCTCTCTGGGGAGAAGCAGA; GGATCAGCCGAGGGTATGATTTTGCTGCGGTCCTCGAATGGTTTGCTGAGCGGGTGGACCGAATTATCCT; ACTCTTCGACGCCCACAAGCTGGACATCTCCGATGAGTTCTCAGAAGTCATCAAGGCTCTCAAGAACCAT; GAGGACAAGATGCGCGTAGTGTTGAACAAAGCTGACCAGATCGAGACCCAGCAGCTGATGCGAGTATACG; GAGCCCTCATGTGGTCCCTGGGGAAGATCGTGAACACCCCCGAGGTGATCCGGGTCTACATTGGCTCCTT; CTGGTCCCACCCACTCCTCATTCCCGACAACCGGAAGCTCTTTGAAGCTGAAGAGCAAGACTTGTTCAGA; GACATTCAGAGTCTACCCCGTAATGCTGCTCTTCGAAAGCTCAACGATCTCATCAAGAGAGCCCGGCTGG; CCAAGGTCCACGCCTACATCATCAGCTCCTTGAAGAAGGAGATGCCCTCAGTGTTTGGGAAGGACACCAA; AAAGAAAGAACTGGTGAACAACCTGGCTGAGATCTATGGCCGGATTGAGCGAGAACACCAGATCTCCCCT; GGAGACTTCCCCAACCTGAAGAGGATGCAGGACCAGCTGCAGGCCCAGGACTTCAGCAAATTCCAGCCAC; TGAAGAGCAAGCTGCTGGAAGTGGTTGATGATATGCTGGCTCATGACATTGCCCAGCTCATGGTGCTGGT; GCGCCAGGAAGAGACCCAACGGCCTGTCCAGATGGTGAAGGGCGGAGCATTTGAGGGAACCTTACAAGGC; CCCTTCGGGCACGGCTATGGAGAGGGAGCTGGGGAGGGCATCGATGATGCCGAGTGGGTGGTGGCGCGGG; ACAAGCCTATGTATGATGAGATCTTCTACACCTTATCCCCAGTGGATGGCAAGATCACAGGTGCCAACGC; CAAGAAGGAGATGGTGCGCTCCAAGTTGCCCAACAGCGTGCTGGGCAAGATCTGGAAGCTAGCCGACATT; GACAAGGATGGCATGTTGGATGACGAGGAGTTTGCCCTGGCCAACCACCTTATCAAAGTCAAGCTAGAGG; GGCATGAGCTGCCCAGTGAGCTACCTGCCCACCTCCTCCCTCCATCTAAGAGGAAAGTATCAGAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208579 protein sequence; Red=Cloning site Green=Tags(s); ; MFSWLGNDDRRKKDPEVFQTVSDGLKKLYKTKLLPLEEYYRFHEFHSPALEDADFDNKPMVLLVGQYSTG; KTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDVEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQ; LPNAVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNH; EDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFR; DIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDTKKKELVNNLAEIYGRIEREHQISP; GDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEETQRPVQMVKGGAFEGTLQG; PFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADI; DKDGMLDDEEFALANHLIKVKLEGHELPSELPAHLLPPSKRKVSE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020578
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020578.3
• RefSeq Size: 3658 bp
• RefSeq ORF: 1608 bp
• Locus ID: 57440
• UniProt ID: Q9QXY6
• Cytogenetics: 17 45.2 cM
• MW: 60.8 kDa
• Summary: ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes (By similarity). Binding to phosphatidic acid induces its membrane tubulation activity (PubMed:26896729). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology (By similarity). Involved in the recycling of internalized D1 dopamine receptor (By similarity). Plays a role in cardiac protein trafficking probably implicating ANK2. Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte excitability and cardiac conduction (PubMed:20489164, PubMed:24759929, PubMed:25825486). In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function (PubMed:21408024). Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance (By similarity). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing. Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with Ehd1 (PubMed:25686250).[UniProtKB/Swiss-Prot Function]