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Yars (NM_134151) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208462
Yars (Myc-DDK-tagged) - Mouse tyrosyl-tRNA synthetase (Yars)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $492.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Yars
Synonyms AL024047; AU018965
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208462 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGATGCTCCAAGCCCTGAGGAGAAGCTGCATCTTATCACCCGGAACCTGCAGGAGGTTCTGGGGG
AAGAGAAGCTGAAGGAGATCCTGAAGGAGCGGGAACTTAAAGTTTACTGGGGCACGGCCACCACGGGCAA
GCCACACGTGGCTTACTTTGTACCCATGTCCAAGATCGCTGACTTCTTGAAGGCAGGGTGTGAGGTAACC
ATCCTGTTTGCGGACCTCCATGCATACCTGGACAACATGAAAGCCCCCTGGGAGCTTCTAGAACTTCGAA
CCAGTTACTATGAGAATGTGATCAAGGCCATGCTGGAGAGTATTGGCGTGCCCTTGGAGAAGCTCAAGTT
TATCAAAGGCACCGACTACCAGCTCAGCAAAGAGTACACACTGGATGTGTACCGACTGTCCTCTGTGGTC
ACACAACATGACGCCAAGAAAGCGGGGGCTGAGGTTGTGAAGCAGGTGGAACACCCTTTGCTGAGTGGTC
TGCTGTACCCCGGTCTGCAGGCTTTGGATGAAGAGTACTTAAAAGTAGACGCCCAGTTCGGCGGTGTTGA
TCAGAGGAAGATTTTTACCTTTGCAGAGAAGTATCTTCCTGCACTTGGCTACTCAAAACGGGTCCACCTG
ATGAATCCCATGGTCCCTGGGTTAACTGGGAGCAAAATGAGCTCGTCAGAAGAGGAGTCCAAAATCGACC
TCCTTGATCGGAAGGAGGACGTGAAGAAAAAGCTGAAGAAGGCCTTCTGTGAGCCCGGCAATGTGGAGAA
CAACGGGGTTCTGTCCTTCATCAAGCATGTCCTCTTTCCCCTCAAGTCTGAGTTTGTGATCCTGAGAGAT
GAGAAGTGGGGTGGAAACAAGACCTACACCGTTTACCTAGAGCTGGAGAAGGACTTCGCTGCTGAGGTCG
TACACCCTGGAGACCTAAAGAATTCTGTTGAAGTCGCCCTGAACAAGTTGCTGGACCCCATTAGAGAGAA
GTTTAATACTCCAGCCCTGAAGAAGCTGGCCAGTGCTGCCTACCCAGATCCTTCAAAGCAGAAGCCTCCT
GCCAAAGGCCCTGCCAAGAACTCAGAACCAGAGGAAGTCATCCCATCCCGGCTGGATATCCGTGTGGGCA
AAATCCTCAGCGTGGAGAAGCACCCAGATGCAGATAGCCTTTATGTGGAGAAGATTGATGTGGGGGAAGC
TGAACCCAGGACTGTGGTGAGCGGCCTCGTACAGTTTGTGCCCAAAGAAGAACTGCAGGACAGGCTGGTG
GTGGTGCTGTGCAATCTGAAACCCCAGAAGATGAGAGGCGTGGACTCGCAGGGCATGCTACTGTGTGCTT
CTGTAGAAGGGGTGAGCCGCCAGGTTGAACCTCTGGACCCTCCTGCTGGCTCTGCTCCTGGTGAACGAGT
GTTCGTACAGGGCTATGAGAAGGGCCAGCCAGATGAGGAGCTCAAGCCCAAGAAGAAAGTCTTTGAGAAG
CTGCAGGCCGACTTTAAAATTTCTGAGGAGTGCATCGCACAGTGGAAGCAAACCAACTTCATGACCAAGC
TGGGATTCGTCTCCTGTAAATCACTAAAAGGGGGTAACATCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208462 protein sequence
Red=Cloning site Green=Tags(s)

MGDAPSPEEKLHLITRNLQEVLGEEKLKEILKERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVT
ILFADLHAYLDNMKAPWELLELRTSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVV
TQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYSKRVHL
MNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRD
EKWGGNKTYTVYLELEKDFAAEVVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYPDPSKQKPP
AKGPAKNSEPEEVIPSRLDIRVGKILSVEKHPDADSLYVEKIDVGEAEPRTVVSGLVQFVPKEELQDRLV
VVLCNLKPQKMRGVDSQGMLLCASVEGVSRQVEPLDPPAGSAPGERVFVQGYEKGQPDEELKPKKKVFEK
LQADFKISEECIAQWKQTNFMTKLGFVSCKSLKGGNIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_134151
ORF Size 1584 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134151.1
RefSeq Size 2874 bp
RefSeq ORF 1695 bp
Locus ID 107271
UniProt ID Q91WQ3
Cytogenetics 4 D2.2
MW 59.1 kDa
Summary Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_134151" in other vectors (4)
SKU Description Size Price
MC202144 Yars (untagged) - Mouse tyrosyl-tRNA synthetase (Yars), (10ug) 10 ug
$492.00
MG208462 Yars (tGFP-tagged) - Mouse tyrosyl-tRNA synthetase (Yars) 10 ug
$692.00
MR208462L3 Lenti ORF clone of Yars (Myc-DDK-tagged) - Mouse tyrosyl-tRNA synthetase (Yars) 10 ug
$792.00
MR208462L4 Lenti ORF clone of Yars (mGFP-tagged) - Mouse tyrosyl-tRNA synthetase (Yars) 10 ug
$792.00

Plasmid Map for MR208462

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208462
Circular map for MR208462

file-alt Citations

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