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Txnrd2 (NM_013711) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208458
Txnrd3 (Myc-DDK-tagged) - Mouse thioredoxin reductase 3 (Txnrd3), transcript variant 2, (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)
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ORF Plasmid:
  $544.00
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Specifications
Specifications
Product Data
Type Mouse Untagged ORF Clone
Target Symbol Txnrd2
Synonyms AA118373; ESTM57301; ESTM573010; TG; TGR; TR; Tr3; Trxr2; Trxrd2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013711
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013711.3
RefSeq Size 1922 bp
RefSeq ORF 1584 bp
Locus ID 26462
UniProt ID Q9JLT4
Cytogenetics 16 11.41 cM
Summary The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Jun 2017
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"NM_013711" in other vectors (1)
SKU Description Size Price
MG208458 Txnrd2 (GFP-tagged) - Mouse thioredoxin reductase 2 (Txnrd2), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) 10 ug
$744.00

Plasmid Map for MR208458

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Cloning scheme for MR208458
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