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Glyctk (NM_174846) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208380
Glyctk (Myc-DDK-tagged) - Mouse glycerate kinase (Glyctk), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $488.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Glyctk or NM_174846"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Glyctk
Synonyms 6230410P16Rik; C430006L12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208380 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGCCCTACAGGTCTTGCCCTGTCTCCTCCGAGCCCCGTCCCGTCCACTCCTCTGGGGGCCCC
CAGTGGCCCGGATGACCAGTGGTATGGCTTTGGCAGAACAGGCACGGCAGCTGTTTGATAGTGCAGTGGG
TGCTGTGCAGCCAGGCCCCATGCTGCAGAGGACACTGTCCCTGGATCCTAGTGGCAGACAGCTGAAGGTG
CGGGACCGGACCTTCCAGCTGCGGGAAAACCTCTACCTAGTGGGCTTCGGCAAGGCCGTACTGGGCATGG
CAGCTGCTGCCGAGGAGCTGCTGGCTCAGCATCTTGTGCAGGGTGTGATCAGCGTACCCAAGGGGATCCG
AGCCGCTATGGAACATGCTGGAAAGAAAGAGATGCTGCTGAAGCCACACAGCCGCGTCCAGGTGTTCGAG
GGCGCCGAGGACAACTTACCGGATCGGGATGCACTCCGGGCCGCGCTGACCATCCAGCAGCTGGCAGAGG
GGCTCACTGCAGATGACCTGCTGCTGGTGCTCATCTCAGGTGGGGGCTCAGCCCTGCTGCCTGCTCCAAT
CCCACCTGTCACACTGGAGGAGAAACAGATGCTCACCAAGCTGCTGGCAGCCCGAGGAGCCACGATCCAG
GAGCTGAACACCATCCGCAAGGCCCTGTCGCAGCTGAAGGGTGGAGGGCTGGCTCAGGCTGCCTATCCTG
CTCAGGTGATAAGCCTCATCTTGTCAGATGTGATTGGGGACCCTTTGGAAGTGATTGCCAGTGGCCCTAC
GGTGGCCAGCGCCCACAGTGTGCAAGACTGCCTGCATATTCTCAACCACTATGGCCTCCGTGCTGCTCTT
CCCCGCTCTGTGAAGACTGTGCTCTCCCGAGCTGACTCTGACCCCCATGGACCACACACCTGTGGCCACG
TCCTCAATGTGATCATCGGCTCTAACTCTCTGGCGCTGGCTGAGGCTCAGAGGCAAGCTGAGGTGCTGGG
CTACCATGCCATGGTGTTGAGCACAGCCATGCAGGGTGATGTAAAAAGAGTGGCTCGGTTCTACGGGCTG
CTAGCCCGGGTGGCTGCAGCCCACCTCACTCCATCTTTGGCTGAGCGTCCCTTGGAGGAGGAGGCGGAAC
TCCATCAGCTGGCAGCTGAGCTCCAGCTCCCGGACCTGCAGCTGGAGGAGGCTCTGGAAGCGGTGGCAAA
GGCTAAAGGGCCGGTCTGCCTCCTGGCTGGTGGTGAGCCCACGGTGCAGCTGCAGGGATCTGGCAAGGGC
GGTCGGAACCAAGAACTGGCCTTGCACGTGGGAGTGGAGCTGGGAAGGCAGCCACTGGGACCTATTGATG
TGCTATTTTTGAGTGGAGGCACTGATGGGCAGGATGGGCCTACAAAGGTGGCCGGGGCCTGGGTCATGTC
TGATCTCATCAGCCAGGCTTCTGCTGAGAGCCTGGACATCGCCACCTCCCTCACCAACAACGACTCATAC
ACTTTCTTTTGCCGCTTCCGGGGAGGAACACATTTGCTGCACACAGGGTTGACAGGGACCAATGTTATGG
ATGTCCACCTCCTGATTCTTCATCCTCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208380 protein sequence
Red=Cloning site Green=Tags(s)

MAAALQVLPCLLRAPSRPLLWGPPVARMTSGMALAEQARQLFDSAVGAVQPGPMLQRTLSLDPSGRQLKV
RDRTFQLRENLYLVGFGKAVLGMAAAAEELLAQHLVQGVISVPKGIRAAMEHAGKKEMLLKPHSRVQVFE
GAEDNLPDRDALRAALTIQQLAEGLTADDLLLVLISGGGSALLPAPIPPVTLEEKQMLTKLLAARGATIQ
ELNTIRKALSQLKGGGLAQAAYPAQVISLILSDVIGDPLEVIASGPTVASAHSVQDCLHILNHYGLRAAL
PRSVKTVLSRADSDPHGPHTCGHVLNVIIGSNSLALAEAQRQAEVLGYHAMVLSTAMQGDVKRVARFYGL
LARVAAAHLTPSLAERPLEEEAELHQLAAELQLPDLQLEEALEAVAKAKGPVCLLAGGEPTVQLQGSGKG
GRNQELALHVGVELGRQPLGPIDVLFLSGGTDGQDGPTKVAGAWVMSDLISQASAESLDIATSLTNNDSY
TFFCRFRGGTHLLHTGLTGTNVMDVHLLILHPQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_174846
ORF Size 1569 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174846.3
RefSeq Size 4226 bp
RefSeq ORF 1572 bp
Locus ID 235582
UniProt ID Q8QZY2
Cytogenetics 9 F1
MW 55.3 kDa
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Documents
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"NM_174846" in other vectors (4)
SKU Description Size Price
MC204143 Glyctk (untagged) - Mouse glycerate kinase (Glyctk), transcript variant 1, (10ug) 10 ug
$488.00
MG208380 Glyctk (tGFP-tagged) - Mouse RIKEN cDNA 6230410P16 gene (6230410P16Rik), transcript variant 1 10 ug
$688.00
MR208380L3 Lenti ORF clone of Glyctk (Myc-DDK-tagged) - Mouse glycerate kinase (Glyctk), transcript variant 1 10 ug
$788.00
MR208380L4 Lenti ORF clone of Glyctk (mGFP-tagged) - Mouse glycerate kinase (Glyctk), transcript variant 1 10 ug
$788.00

Plasmid Map for MR208380

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208380
Circular map for MR208380

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