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Slc1a1 (NM_009199) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208367
Slc1a1 (Myc-DDK-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $487.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc1a1
Synonyms D130048G10Rik; EAAC1; EAAC2; EAAT3; MEAAC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208367 representing NM_009199
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGCCCACGAGCTCGGGATGTGACTGGCGCCGCTTCCTACGGAATCACTGGCTGCTGCTCTCCA
CCGTGGCCGCCGTGGTACTAGGAATTGTCTTAGGAGTCGTGGTTCGAGGACACAGTGAGCTCTCAAATCT
GGATAAATTCTACTTTGCTTTTCCTGGGGAAATTCTGATGAGGATGCTGAAGCTGGTCATTTTGCCGCTG
ATCGTATCCAGCATGATCACAGGTGTCGCTGCACTGGATTCCAATGTGTCTGGGAAGATTGGTCTGCGCG
CTGTAGTATATTATTTCTCCACCACCGTCATTGCTGTAATCCTAGGTATTGTGTTAGTTGTGAGTATCAA
GCCTGGTGTCACTCAGAAAGTGAATGACATCAACAGGACGGGTAAAACCCCTGAAGTCAGCACCATGGAT
GCCATGTTGGACCTGATCAGGAACATGTTCCCTGAGAATCTGGTCCAAGCCTGTTTTCAGCAGTACAAAA
CCAAGCGGGAAGAGGTGAAGCCTGTGGGCGATCCTGGGGGGAACGCAACGGAGGTGTCTGTCACCACAGC
CATGACAACAATGTCTGAGAACAAGACAAAGGAATACAAGATCGTGGGCCTGTACTCAGACGGCATCAAT
GTCCTGGGCTTGATTATCTTCTGCCTCGTCTTTGGCCTTGTCATTGGGAAAATGGGAGAAAAGGGGCAGA
TTCTGGTGGACTTCTTCAATGCCTTGAGTGACGCCACCATGAAAATCGTCCAGATCATCATGTGCTACAT
GCCGATTGGCATTTTGTTCCTAATTGCTGGGAAGATCATAGAAGTTGAAGACTGGGAAATATTCCGCAAG
CTGGGCCTTTACATGGCCACTGTCCTGAGCGGGCTTGCAATCCACTCCCTCATAGTTCTGCCCCTGCTCT
ATTTCATAGTTGTGCGGAAGAACCCTTTCCGCTTTGCCTTGGGTATGGCGCAGGCTCTCCTGACAGCTCT
CATGATCTCGTCCAGTTCGGCAACCCTGCCAGTTACATTCCGCTGTGCGGAAGAAAAGAACCAGGTAGAC
AAGAGGATCACGAGATTTGTGCTGCCTGTTGGTGCCACCATCAACATGGACGGCACTGCGCTCTACGAAG
CTGTGGCAGCCGTGTTTATTGCGCAACTGAATGGCTTGGACCTAAGCATTGGGCAGATCGTCACCATCAG
CATTACAGCCACCGCTGCCAGCATTGGAGCTGCTGGGGTGCCCCAGGCTGGCCTGGTGACCATGGTGATC
GTGCTGAGTGCTGTGGGGCTGCCTGCCGAGGACGTCACCCTGATCATTGCTGTTGACTGGCTCCTGGACC
GGTTCAGGACCATGGTGAACGTCCTGGGTGATGCGTTTGGGACGGGCATCGTAGAGAAGCTCTCGAAGAA
GGAGCTGGAGCAGATGGATGTTTCGTCTGAAGTCAACATCGTGAACCCCTTTGCCTTGGAACCCACAACC
CTCGATAACGAAGACTCAGATACCAAGAAGTCTTATGTCAATGGGGGCTTCGCGGTAGACAAATCTGACA
CCATCTCGTTCACTCAGACCTCACAGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208367 representing NM_009199
Red=Cloning site Green=Tags(s)

MGKPTSSGCDWRRFLRNHWLLLSTVAAVVLGIVLGVVVRGHSELSNLDKFYFAFPGEILMRMLKLVILPL
IVSSMITGVAALDSNVSGKIGLRAVVYYFSTTVIAVILGIVLVVSIKPGVTQKVNDINRTGKTPEVSTMD
AMLDLIRNMFPENLVQACFQQYKTKREEVKPVGDPGGNATEVSVTTAMTTMSENKTKEYKIVGLYSDGIN
VLGLIIFCLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPIGILFLIAGKIIEVEDWEIFRK
LGLYMATVLSGLAIHSLIVLPLLYFIVVRKNPFRFALGMAQALLTALMISSSSATLPVTFRCAEEKNQVD
KRITRFVLPVGATINMDGTALYEAVAAVFIAQLNGLDLSIGQIVTISITATAASIGAAGVPQAGLVTMVI
VLSAVGLPAEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALEPTT
LDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009199
ORF Size 1569 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009199.3
RefSeq Size 3708 bp
RefSeq ORF 1572 bp
Locus ID 20510
UniProt ID P51906
Cytogenetics 19 C1
MW 57.1 kDa
Summary Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:12119102, PubMed:18684713). Can also transport L-cysteine (PubMed:30840898). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:9233792). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (PubMed:9233792). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (PubMed:30840898). Negatively regulated by ARL6IP5 (PubMed:12119102).UniProtKB/Swiss-Prot Function
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"NM_009199" in other vectors (4)
SKU Description Size Price
MC205239 Slc1a1 (untagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1), (10ug) 10 ug
$488.00
MG208367 Slc1a1 (tGFP-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) 10 ug
$489.00 $687.00
MR208367L3 Lenti ORF clone of Slc1a1 (Myc-DDK-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) 10 ug
$787.00
MR208367L4 Lenti ORF clone of Slc1a1 (mGFP-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) 10 ug
$787.00

Plasmid Map for MR208367

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208367
Circular map for MR208367

file-alt Citations

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