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Rcc2 (NM_173867) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208331
Rcc2 (Myc-DDK-tagged) - Mouse regulator of chromosome condensation 2 (Rcc2)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $484.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rcc2
Synonyms 2610510H01Rik; 2610529N02Rik; AA536646; AA675016; mKIAA1470; Td60
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208331 representing NM_173867
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAGGAAGAAGGGGGCGGCCTGGGAGGAGCCGAGTTCGGGCAACGGCACGGCGCGCGCGGGGCCCC
GGCGACGCGGCGGCCCCGCGGGCAGGAAGCGCGAGCGGCCCGAGCGCTGCAGCAGCAGCAGCGGCGGCGG
CAGCAGCGGCGACGAGGACGGCCCGGAGCTGGACGGGGCCCCCGGCGGCGGCAAGCGCACAGCCAGGCCG
GCGACGGCGGGCAAGGCGGCCGGCGCGGCCGCGATCATCACGGAGCCCGAGCACACGAAGGAGCGCGTCA
AACTTGAAGGGTCTAAGTGCAAAGGGCAGCTTTTGATTTTTGGGGCAACCAACTGGGACTTGATTGGACG
GAAGGAAGTGCCTAAACAGCAAGCTGCGTACCGCAACCTTGGTCAGAATTTGTGGGGGCCCCATAGGTAT
GGGTGCCTGTCAGGAGTCCGGGTGCGAACCGTGGTTTCAGGCTCATGTGCTGCCCACAGCCTCCTCATCA
CCACAGAAGGGAAGCTGTGGAGCTGGGGTCGGAATGAGAAGGGGCAGCTGGGCCATGGTGATACCAAGAG
GGTGGAAGCCCCCAGACTCATCGAGGCCCTCAGCCATGAGGCAATCGTGCTGGCAGCCTGTGGGCGCAAC
CACACCCTGGCCTTGACAGATACTGGATCAGTGTTTGCCTTCGGAGAGAATAAAATGGGGCAGCTGGGTC
TCGGGAACCAGACAGATGCCGTCCCAAGTCCTGCTCAGATAATGTACAACGGGCAGCCAATCACCAAGAT
GGCCTGCGGGGCCGAGTTCAGCATGCTGATGGACTGTAAAGGAAACCTCTACTCCTTTGGGTGCCCTGAG
TATGGCCAGCTGGGGCACAACTCAGATGGCAAGTTCATCGCCCGGGCGCAGAGGATAGAATATGATTGTG
AGCTGGTGCCCCGCAGAGTGGCCATCTTCATCGAGAAGACCAAGGACGGGCAGATCTTACCTGTCCCCAA
CGTGGTGGTTCGAGATGTAGCCTGTGGCGCTAACCACACACTGGTCCTGGACTCTCAGAAGCGAGTGTTC
TCCTGGGGCTTTGGTGGCTATGGCCGGCTGGGCCATGCCGAGCAGAAGGATGAGATGGTGCCTCGCCTGG
TGAAGCTGTTCGACTTCCCGGGGCGTGGTGCAACCCAGATCTATGCCGGCTACACCTGCTCCTTCGCTGT
CAGTGAAGTGGGTGGCCTGTTTTTCTGGGGGGCCACCAATACCTCCCGAGAGTCTACCATGTACCCGAAG
GCAGTGCAGGACCTCTGCGGCTGGCGGATCCGGAGCCTTGCCTGCGGGAAGAGCAGCATCATCGTAGCTG
CGGACGAGAGTACTATCAGTTGGGGTCCATCGCCAACCTTCGGGGAATTGGGGTATGGGGACCACAAGCC
CAAGTCTTCTACTGCGGCCCAGGAGGTGAAGACTCTGGATGGCATCTTCTCCGAACAGGTGGCCATGGGC
TATTCACACTCCTTGGTGATAGCGCGAGACGAGAGCGAGGCTGAGAAGGAGAAGCTGCAGAGGCTACCTG
AGTACACCCCGCGCACCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208331 representing NM_173867
Red=Cloning site Green=Tags(s)

MPRKKGAAWEEPSSGNGTARAGPRRRGGPAGRKRERPERCSSSSGGGSSGDEDGPELDGAPGGGKRTARP
ATAGKAAGAAAIITEPEHTKERVKLEGSKCKGQLLIFGATNWDLIGRKEVPKQQAAYRNLGQNLWGPHRY
GCLSGVRVRTVVSGSCAAHSLLITTEGKLWSWGRNEKGQLGHGDTKRVEAPRLIEALSHEAIVLAACGRN
HTLALTDTGSVFAFGENKMGQLGLGNQTDAVPSPAQIMYNGQPITKMACGAEFSMLMDCKGNLYSFGCPE
YGQLGHNSDGKFIARAQRIEYDCELVPRRVAIFIEKTKDGQILPVPNVVVRDVACGANHTLVLDSQKRVF
SWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGATQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMYPK
AVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVAMG
YSHSLVIARDESEAEKEKLQRLPEYTPRTL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173867
ORF Size 1560 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173867.5, NP_776292.1
RefSeq Size 3790 bp
RefSeq ORF 1563 bp
Locus ID 108911
UniProt ID Q8BK67
Cytogenetics 4 D3
MW 56 kDa
Summary Multifunctional protein that may effect its functions by regulating the activity of small GTPases, such as RAC1 and RALA. Required for normal progress through the cell cycle, both during interphase and during mitosis. Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles. Required for normal organization of the microtubule cytoskeleton in interphase cells. Functions as guanine nucleotide exchange factor (GEF) for RALA. Interferes with the activation of RAC1 by guanine nucleotide exchange factors (By similarity). Prevents accumulation of active, GTP-bound RAC1, and suppresses RAC1-mediated reorganization of the actin cytoskeleton and formation of membrane protrusions (PubMed:25074804). Required for normal cellular responses to contacts with the extracellular matrix of adjacent cells, and for directional cell migration in response to a fibronectin gradient (in vitro) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_173867" in other vectors (3)
SKU Description Size Price
MC217502 Rcc2 (untagged) - Mouse regulator of chromosome condensation 2 (Rcc2), (10ug) 10 ug
$533.00
MR208331L3 Lenti ORF clone of Rcc2 (Myc-DDK-tagged) - Mouse regulator of chromosome condensation 2 (Rcc2) 10 ug
$784.00
MR208331L4 Lenti ORF clone of Rcc2 (mGFP-tagged) - Mouse regulator of chromosome condensation 2 (Rcc2) 10 ug
$784.00

Plasmid Map for MR208331

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208331
Circular map for MR208331

file-alt Citations

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