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Ptbp3 (NM_144904) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208328
Ptbp3 (Myc-DDK-tagged) - Mouse ROD1 regulator of differentiation 1 (S. pombe) (Rod1), transcript variant 1
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $485.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ptbp3 or NM_144904"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptbp3
Synonyms 5830471K22Rik; AA407443; AI462022; AW107884; C86549; Rod1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208328 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAGCTCGACTTCTGCAGCTAATGGGAATGACAACAAGAAATTTAAAGGAGATAGACCTCCCTGTT
CGCCTTCCCGTGTTCTCCATCTTCGAAAAATTCCATGTGATGTCACCGAAGCAGAGGTCATATCATTAGG
TCTACCATTTGGCAAAGTAACTAATCTTTTGATGTTGAAAGGAAAAAGCCAGGCCTTTTTAGAAATGGCT
TCTGAAGAAGCTGCTGTTACTATGATAAATTATTACACTCCTGTTACTCCTCATCTTCGAAGTCAGCCTG
TTTATATCCAGTATTCCAATCACCGAGAACTTAAGACTGACAATCTGCCTAATCAGGCTAGAGCCCAAGC
TGCACTGCAGGCTGTCAGTGCAGTCCAGTCAGGAAACTTGTCCCTTCCTGGAGCTACTGCAAATGAAGGC
ACATTGCTACCTGGGCAGAGCCCTGTGCTCCGGATAATTATTGAAAACCTATTTTACCCTGTCACTCTGG
AAGTTCTCCATCAGATATTTTCTAAATTTGGCACGGTGTTGAAGATTATCACCTTTACAAAGAATAATCA
GTTTCAAGCCTTGCTTCAGTATGCCGACCCAGTGAATGCGCAGTATGCCAAAATGGCTCTGGATGGCCAG
AATATCTACAATGCGTGCTGCACTCTCCGTATTGACTTCTCCAAGCTCACCAGCCTTAATGTGAAATACA
ATAATGACAAAAGCAGAGACTTCACTCGCTTAGACCTTCCTACTGGTGACGGCCAGCCATCTCTTGAACC
TCCTATGGCTGCTGCTTTTGGTGCACCGGGTATAATGTCCTCTCCATATGCAGGGGCTGCTGGCTTTGCC
CCAGCCATCGCATTTCCTCAAGCAGCAGGTCTGTCTGTCCCAGCTGTTCCCGGAGCCCTTGGTCCTCTCA
CACTTACCTCCTCTGCTGTCAGTGGAAGGATGGCCATTCCTGGGGCAAGTGGTATGCCTGGGAATTCTGT
TCTGCTGGTCACCAACCTCAATCCTGACTTTATCACACCACATGGGCTTTTTATCCTATTTGGAGTGTAT
GGTGATGTACATCGAGTGAAGATTATGTTTAACAAGAAAGAAAACGCCTTGGTGCAGATGGCGGATGCGA
GTCAAGCCCAGCTAGCAATGAACCACCTAAGTGGTCAGAGGCTCTATGGAAAAGTACTTCGGGCTACACT
GTCCAAACATCAAGCCGTCCAGCTCCCTAGAGAGGGACAGGAAGACCAAGGTCTGACCAAGGACTTCAGC
AATAGTCCCCTGCATCGCTTTAAAAAGCCCGGCTCAAAGAATTTCCAAAATATCTTCCCTCCATCAGCCA
CACTTCATCTTTCCAACATTCCTCCTTCTGTCACAATGGATGACCTGAAGAACCTGTTCACAGAAGCTGG
ATGCTCAGTGAAGGCTTTTAAGTTCTTCCAGAAAGACCGTAAGATGGCTCTCATTCAGTTGGGATCTGTG
GAAGAAGCAATCCAGGCTCTTATTGAGCTTCACAATCATGACCTTGGAGAGAATCACCACCTCAGAGTTT
CCTTCTCAAAATCCACCATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208328 protein sequence
Red=Cloning site Green=Tags(s)

MNSSTSAANGNDNKKFKGDRPPCSPSRVLHLRKIPCDVTEAEVISLGLPFGKVTNLLMLKGKSQAFLEMA
SEEAAVTMINYYTPVTPHLRSQPVYIQYSNHRELKTDNLPNQARAQAALQAVSAVQSGNLSLPGATANEG
TLLPGQSPVLRIIIENLFYPVTLEVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVNAQYAKMALDGQ
NIYNACCTLRIDFSKLTSLNVKYNNDKSRDFTRLDLPTGDGQPSLEPPMAAAFGAPGIMSSPYAGAAGFA
PAIAFPQAAGLSVPAVPGALGPLTLTSSAVSGRMAIPGASGMPGNSVLLVTNLNPDFITPHGLFILFGVY
GDVHRVKIMFNKKENALVQMADASQAQLAMNHLSGQRLYGKVLRATLSKHQAVQLPREGQEDQGLTKDFS
NSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVTMDDLKNLFTEAGCSVKAFKFFQKDRKMALIQLGSV
EEAIQALIELHNHDLGENHHLRVSFSKSTI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144904
ORF Size 1560 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144904.1
RefSeq Size 6880 bp
RefSeq ORF 1656 bp
Locus ID 230257
UniProt ID Q8BHD7
Cytogenetics 4 B3
MW 56.4 kDa
Summary RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes Fas exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144904" in other vectors (3)
SKU Description Size Price
MC200715 Ptbp3 (untagged) - Mouse ROD1 regulator of differentiation 1 (S. pombe) (Rod1), transcript variant 1, (10ug) 10 ug
$485.00
MR208328L3 Lenti ORF clone of Ptbp3 (Myc-DDK-tagged) - Mouse ROD1 regulator of differentiation 1 (S. pombe) (Rod1), transcript variant 1 10 ug
$785.00
MR208328L4 Lenti ORF clone of Ptbp3 (mGFP-tagged) - Mouse ROD1 regulator of differentiation 1 (S. pombe) (Rod1), transcript variant 1 10 ug
$785.00

Plasmid Map for MR208328

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR208328
Circular map for MR208328

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