Hsf2 (NM_008297) Mouse Tagged ORF Clone
$289.00
$482.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Hsf2 |
| Synonyms | AI661205 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008297 |
| ORF Size | 1551 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008297.1, NM_008297.2, NM_008297.3, NP_032323.2 |
| RefSeq Size | 2623 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 15500 |
| UniProt ID | P38533 |
| Cytogenetics | 10 B4 |
| MW | 58.2 kDa |
| Summary | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008297" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG208286 | Hsf2 (tGFP-tagged) - Mouse heat shock factor 2 (Hsf2) | 10 ug |
$682.00
|
|
| MR208286L3 | Lenti ORF clone of Hsf2 (Myc-DDK-tagged) - Mouse heat shock factor 2 (Hsf2) | 10 ug |
$782.00
|
|
| MR208286L4 | Lenti ORF clone of Hsf2 (mGFP-tagged) - Mouse heat shock factor 2 (Hsf2) | 10 ug |
$782.00
|
|
Citations
Decreased ZO1 expression causes loss of time-dependent tight junction function in the liver of ob/ob mice
Tsurudome, Morita, Horiguchi et al
Research Square (2021)
Tsurudome, Morita, Horiguchi et al
Research Square (2021)
Showing 1-1 of 1 papers.
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