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Gpr176 (NM_201367) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208272
Gpr176 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 176 (Gpr176)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $479.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr176
Synonyms Gm1012
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208272 representing NM_201367
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCACAACAGCAGCTGGGTGTCACCGAACACCAGCCACCCGCGCAACACGTCGGGCGCCGAGGCTG
GCGCCAACCTCAGCGCGTTCGGGGAGCTGAGCGAGGCTCAGCTGTACCGCCAGTTCACCACCACCGTGCA
GGTCGTCATCTTCATTGGCTCGCTACTGGGAAACTTCATGGTGTTATGGTCAACTTGCCGCACAACAGTG
TTCAAATCTGTCACCAACAGGTTCATTAAAAACCTGGCCTGCTCCGGGATTTGTGCCAGTGTGGTCTGTG
TGCCCTTCGACATCATCCTGAGCAGCAGCCCTCACTGTTGCTGGTGGATCTATACGATGCTCTTCTGCAA
GGTGCTCAAATTCCTACACAAGGTGTTCTGCTCTGTGACTGTCCTCAGTTTTCCTGCTATTGCCCTGGAC
AGGTACTATTCAGTCCTTTATCCTCTGGAGAGAAAAATATCTGATGCCAAGTCTCGGGAGCTAGTGATGT
ACATCTGGGCTCATGCAGTGGTGGCCAGTGTCCCTGTGTTTGCTGTGACCAATGTCGCTGACATCTATGC
TATGTCCACCTGCACGGAAGTCTGGAGCAACTCCTTGGGCCACCTGGTGTATGTTCTGATCTACAATGTC
ACCACAGTCATTGTGCCCGTGGCTGTGGTATTTCTCTTTCTCATCCTGATCCGGAGGGCCCTGAGTGCCA
GCCAGAAGAAGAAAGTCATAATTGCTGCACTGAGGACCCCACAGAACACCATCTCTATCCCCTATGCTTC
CCAGCGAGAGGCAGAGCTACATGCCACCCTGCTCTCCATGGTGACAGTTTTCATTCTCTGCAGTGTACCC
TATGCCACCCTGGTTGTCTACCAGACTGTGCTCAATGTACCTAACACCTCTGTCTTCTTGCTTCTCACGG
CCATTTGGCTGCCCAAAGTCTCTCTGTTGGCCAACCCTGTGCTCTTCTTAACTGTGAACAAATCGGTACG
CAAGTGCTTGGTGGGGACCCTGGTGCAGTTGCACCACCGGTACAGTCGCCGCAATGTGGTTAGTACAGGG
AGTGGGGTGGCTGAGCCCAGCCTTGAGCCCAGCATGCGCTCTGGTAGCCAGCTCTTGGAGATGTTCCACA
TTGGGCAGCAACAGATCTTTAAGCCCTCGGAGGATGAGGAAGAAAGTGAGGCCAAGTATCTCGGCTCAGC
CGATTTCCAGGCAAAGGAGGTGCTCACTTCCTGCCCAGAGGGCGAGCAGGAGCCACCACAGCTTGCACCG
TCTGTGCCACCTCCAGGCACAGTGGACTCTGAACCTCGGGTGTCCCCAGTAGCTCCCATGGAATCTGGGA
TATTTCCTGATAAGTATTCCCTGCAGTTTGGCTTTGGGCCTTTTGAGTTACCCCCTCAGTGGCTCTCTGA
GACCCGAAATAGCAAGAAGAGGCTGCTTCCCCCACTGGGCAATACCCCAGAAGAGCTGATCCAGACAAAG
GTGCCCAGAGTGAACAGGGTGGAACGGAAGATGAGCAGAAACAATAAAGTGAGCATTTTTCCAAAGGTAG
ACTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208272 representing NM_201367
Red=Cloning site Green=Tags(s)

MGHNSSWVSPNTSHPRNTSGAEAGANLSAFGELSEAQLYRQFTTTVQVVIFIGSLLGNFMVLWSTCRTTV
FKSVTNRFIKNLACSGICASVVCVPFDIILSSSPHCCWWIYTMLFCKVLKFLHKVFCSVTVLSFPAIALD
RYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYAMSTCTEVWSNSLGHLVYVLIYNV
TTVIVPVAVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVTVFILCSVP
YATLVVYQTVLNVPNTSVFLLLTAIWLPKVSLLANPVLFLTVNKSVRKCLVGTLVQLHHRYSRRNVVSTG
SGVAEPSLEPSMRSGSQLLEMFHIGQQQIFKPSEDEEESEAKYLGSADFQAKEVLTSCPEGEQEPPQLAP
SVPPPGTVDSEPRVSPVAPMESGIFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTK
VPRVNRVERKMSRNNKVSIFPKVDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201367
ORF Size 1545 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201367.3
RefSeq Size 3791 bp
RefSeq ORF 1548 bp
Locus ID 381413
UniProt ID Q80WT4
Cytogenetics 2 E5
MW 57.6 kDa
Summary Orphan receptor involved in normal circadian rhythm behavior (PubMed:26882873). Acts through the G-protein subclass G(z)-alpha and has an agonist-independent basal activity to repress cAMP production (PubMed:26882873).UniProtKB/Swiss-Prot Function
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"NM_201367" in other vectors (4)
SKU Description Size Price
MC203588 Gpr176 (untagged) - Mouse G protein-coupled receptor 176 (Gpr176), (10ug) 10 ug
$480.00
MG208272 Gpr176 (tGFP-tagged) - Mouse G protein-coupled receptor 176 (Gpr176) 10 ug
$489.00 $679.00
MR208272L3 Lenti ORF clone of Gpr176 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 176 (Gpr176) 10 ug
$779.00
MR208272L4 Lenti ORF clone of Gpr176 (mGFP-tagged) - Mouse G protein-coupled receptor 176 (Gpr176) 10 ug
$779.00

Plasmid Map for MR208272

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208272
Circular map for MR208272

file-alt Citations

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