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Atp6v1b2 (NM_007509) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208205
Atp6v1b2 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B2 (Atp6v1b2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $477.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Atp6v1b2 or NM_007509"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atp6v1b2
Synonyms AI194269; AI790362; Atp6b2; HO57; R74844
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208205 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTGCGAGCGATGCGGGGAATCGTGAACGGGGCCGCACCCGAACTGCCCGTGCCCACCGGTGGGC
CGATGGCCGGAGCTCGGGAGCAGGCGCTGGCGGTGAGCCGGAACTACCTATCCCAGCCTCGTCTCACCTA
CAAGACTGTCTCTGGGGTGAATGGTCCACTAGTGATCCTAGATCATGTGAAGTTTCCCAGATACGCTGAG
ATTGTCCACTTGACATTACCAGATGGCACAAAGAGAAGTGGGCAAGTCCTAGAAGTTAGTGGCTCCAAAG
CAGTGGTTCAGGTATTTGAAGGGACATCTGGTATAGACGCCAAGAAAACATCCTGTGAGTTTACTGGAGA
TATTCTCCGAACACCAGTGTCTGAGGATATGCTTGGTCGAGTATTCAACGGATCAGGAAAACCCATTGAC
CGAGGCCCTGTGGTGCTGGCTGAAGACTTCCTTGACATCATGGGTCAGCCAATCAACCCTCAGTGTCGGA
TCTATCCAGAAGAGATGATTCAGACGGGCATTTCCGCCATCGATGGCATGAACAGTATTGCTAGGGGACA
GAAAATCCCCATCTTTTCTGCTGCTGGATTACCACACAACGAGATTGCAGCTCAGATCTGTCGCCAGGCT
GGTTTGGTAAAGAAATCCAAAGATGTAGTGGACTATAGTGAAGAAAATTTTGCCATTGTGTTTGCTGCTA
TGGGAGTAAACATGGAAACAGCCCGGTTCTTTAAATCTGACTTTGAAGAAAATGGCTCAATGGACAATGT
CTGCCTTTTTTTGAATCTGGCTAATGACCCAACCATTGAGCGGATCATCACTCCTCGCCTGGCTCTGACC
ACGGCCGAGTTTCTGGCATATCAGTGTGAGAAGCACGTGCTGGTGATCCTGACGGACATGAGCTCCTACG
CTGAAGCGCTGCGAGAGGTTTCAGCTGCCAGGGAAGAGGTCCCTGGTCGGCGAGGCTTCCCAGGCTACAT
GTATACCGACTTAGCCACAATCTATGAACGTGCCGGTCGAGTGGAAGGTAGAAACGGCTCTATTACCCAA
ATCCCTATTCTCACCATGCCCAATGACGATATCACTCATCCCATCCCTGACTTGACTGGGTACATTACTG
AGGGCCAGATCTATGTGGACAGACAGCTGCACAACAGACAGATTTACCCTCCTATTAATGTGCTGCCCTC
ACTCTCTCGGTTAATGAAGTCAGCTATCGGAGAAGGAATGACCAGAAAGGATCACGCTGATGTGTCTAAC
CAGTTGTATGCGTGCTATGCCATCGGTAAGGACGTGCAAGCCATGAAAGCCGTGGTGGGAGAGGAAGCCC
TGACCTCAGATGATCTCCTTTACCTGGAATTTCTGCAGAAGTTTGAGAAGAACTTCATTACTCAGGGTCC
CTATGAAAACCGAACTGTTTATGAGACTTTGGACATTGGCTGGCAGTTGCTTCGAATCTTCCCCAAAGAA
ATGCTGAAGAGAATCCCTCAGAGCACCCTGAGCGAATTTTACCCTCGAGACTCTGCAAAACAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208205 protein sequence
Red=Cloning site Green=Tags(s)

MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAE
IVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPID
RGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQA
GLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALT
TAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQ
IPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSN
QLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFEKNFITQGPYENRTVYETLDIGWQLLRIFPKE
MLKRIPQSTLSEFYPRDSAKH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007509
ORF Size 1533 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007509.3
RefSeq Size 2742 bp
RefSeq ORF 1536 bp
Locus ID 11966
UniProt ID P62814
Cytogenetics 8 B3.3
MW 56.6 kDa
Summary Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007509" in other vectors (4)
SKU Description Size Price
MC203853 Atp6v1b2 (untagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B2 (Atp6v1b2), (10ug) 10 ug
$477.00
MG208205 Atp6v1b2 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B2 (Atp6v1b2) 10 ug
$677.00
MR208205L3 Lenti ORF clone of Atp6v1b2 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B2 (Atp6v1b2) 10 ug
$777.00
MR208205L4 Lenti ORF clone of Atp6v1b2 (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B2 (Atp6v1b2) 10 ug
$777.00

Plasmid Map for MR208205

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208205
Circular map for MR208205

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