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Wasl (NM_028459) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208041
Wasl (Myc-DDK-tagged) - Mouse Wiskott-Aldrich syndrome-like (human) (Wasl), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $466.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wasl
Synonyms 2900021I12Rik; 3110031I02Rik; N-WASP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208041 representing NM_028459
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCGGGCCAGCAGCCCCCGCGGAGGGTCACCAACGTGGGCTCCCTGCTGCTCACCCCGCAAGAGA
ACGAGTCTCTCTTCTCCTTCCTCGGCAAGAAATGTGTGACTATGTCTTCAGCAGTGGTGCAGTTGTATGC
AGCAGATCGGAACTGTATGTGGGCAAAGAAGTGCAGTGGTGTCGCTTGTCTGGTTAAGGACAATCCTCAG
AGATCTTATTTTTTAAGAATATTTGACATTAAGGATGGGAAATTACTGTGGGAACAAGAGCTATACAATA
ACTTTGTATATAATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGTAGCTCTTAA
TTTTGCCAATGAAGAAGAAGCAAAAAAGTTCCGAAAAGCAGTTACAGACCTGTTGGGCCGACGACAAAGG
AAATCTGAAAAAAGACGAGATGCTCCAAATGGTCCCAATCTACCCATGGCTACAGTTGACATAAAAAATC
CAGAAATCACAACAAATAGATTTTATGGTTCACAAGTCAACAACATCTCCCACACCAAAGAAAAGAAGAA
AGGAAAAGCTAAAAAGAAGAGATTAACCAAGGCAGATATTGGAACACCAAGTAATTTCCAGCACATTGGG
CATGTTGGATGGGATCCAAATACAGGTTTTGATCTAAATAATTTGGATCCAGAATTAAAGAATCTTTTTG
ATATGTGTGGGATCTCTGAGGCCCAGCTTAAAGACAGAGAAACATCAAAAGTTATTTATGACTTTATTGA
AAAAACAGGAGGTGTAGAAGCTGTTAAAAACGAACTCCGAAGACAAGCACCACCACCACCTCCACCCTCG
AGAGGAGGACCTCCCCCTCCTCCTCCCCCTCCTCATAGCTCAGGTCCTCCTCCCCCTCCTGCCCGAGGAA
GGGGGGCTCCTCCTCCCCCACCTTCAAGAGCTCCTACTGCTGCACCTCCACCTCCACCTCCTTCTAGGCC
TGGTGTTGTTGTTCCTCCGCCCCCTCCAAACAGGATGTACCCTCCTCCACCACCAGCGCTGCCCTCTTCA
GCACCTTCAGGCCCTCCACCACCTCCACCTCCATCTATGGCAGGGTCCACAGCACCACCACCTCCTCCTC
CACCTCCGCCCCCACCAGGGCCACCACCTCCCCCTGGCCTGCCTTCTGATGGTGACCATCAAGTTCCAGC
TCCTTCAGGAAACAAAGCAGCTCTTTTGGATCAAATTAGAGAGGGTGCTCAGCTGAAAAAAGTGGAACAG
AATAGCCGGCCCGTGTCCTGCTCAGGAAGGGATGCGCTTTTAGACCAGATACGACAGGGCATTCAATTGA
AATCTGTGTCTGATGGCCAAGAGTCCACACCACCAACACCCGCACCCACTTCAGGAATTGTGGGTGCGCT
GATGGAAGTGATGCAGAAAAGGAGCAAAGCCATTCATTCCTCAGATGAAGATGAAGACGATGATGATGAA
GAAGATTTTGAGGATGATGATGAGTGGGAAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208041 representing NM_028459
Red=Cloning site Green=Tags(s)

MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQLYAADRNCMWAKKCSGVACLVKDNPQ
RSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQR
KSEKRRDAPNGPNLPMATVDIKNPEITTNRFYGSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIG
HVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPS
RGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPPPPNRMYPPPPPALPSS
APSGPPPPPPPSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPAPSGNKAALLDQIREGAQLKKVEQ
NSRPVSCSGRDALLDQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDE
EDFEDDDEWED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028459
ORF Size 1503 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028459.2, NP_082735.2
RefSeq Size 4348 bp
RefSeq ORF 1506 bp
Locus ID 73178
UniProt ID Q91YD9
Cytogenetics 6 A3.1
MW 54.3 kDa
Summary Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (By similarity). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (PubMed:12871950). Plays a role in dendrite spine morphogenesis (PubMed:25851601).UniProtKB/Swiss-Prot Function
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"NM_028459" in other vectors (3)
SKU Description Size Price
MG208041 Wasl (tGFP-tagged) - Mouse Wiskott-Aldrich syndrome-like (human) (Wasl) 10 ug
$665.00
MR208041L3 Lenti ORF clone of Wasl (Myc-DDK-tagged) - Mouse Wiskott-Aldrich syndrome-like (human) (Wasl), transcript variant 1 10 ug
$766.00
MR208041L4 Lenti ORF clone of Wasl (mGFP-tagged) - Mouse Wiskott-Aldrich syndrome-like (human) (Wasl), transcript variant 1 10 ug
$766.00

Plasmid Map for MR208041

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file-download TXT (FASTA format)

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Cloning scheme for MR208041
Circular map for MR208041

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