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Mef2a (NM_001033713) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207994
Mef2a (Myc-DDK-tagged) - Mouse myocyte enhancer factor 2A (Mef2a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mef2a
Synonyms A430079H05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207994 representing NM_001033713
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCGAAAGAAGATACAAATCACACGCATAATGGATGAGAGGAACCGACAGGTTACTTTTACAAAGC
GAAAGTTTGGATTGATGAAGAAAGCCTATGAACTCAGTGTGCTCTGTGACTGTGAAATAGCACTCATCAT
TTTCAACAGCTCTAATAAGCTGTTTCAGTACGCTAGCACTGACATGGACAAAGTCCTTCTCAAATACACT
GAGTATAACGAGCCTCATGAAAGCAGGACGAACTCGGATATCGTTGAGACGTTAAGAAAGAAAGGCCTTA
ATGGTTGTGAGAGCCCTGATGCTGACGATTACTTTGAGCACAGTCCACTCTCGGAGGACAGATTCAGCAA
ACTAAATGAAGATAGTGATTTTATTTTCAAACGAGGCCCTCCTGGCTTGCCACCTCAGAACTTCTCAATG
TCTGTCACAGTCCCTGTGACCAGCCCTAATGCTTTGTCGTACACTAACCCAGGGAGTTCACTCGTGTCAC
CGTCTTTGGCAGCCAGCTCAACATTAGCAGATTCGAGCATGCTGTCTCCACCCCCAGCCACGCTACATAG
AAATGTGTCCCCAGGAGCTCCGCAGAGACCACCAAGTACCGGCAGTGCAAGTGGGATGTTGAGCACTACA
GACCTCACGGTGCCAAATGGAGCTGGAAATAGTCCTGTGGGGAATGGATTTGTAAACTCAAGGGCCTCTC
CAAATTTGATTGGAAATACTGGTGCAAATAGTTTAGGCAAAGTCATGCCTACAAAGTCTCCCCCGCCACC
AGGTGGTGGCAGTCTTGGAATGAACAGTCGGAAACCAGATCTACGAGTTGTCATTCCCCCATCAAGCAAG
GGCATGATGCCCCCACTTTCGGAGGAAGAGGAATTGGAGTTGAATGCCCAAAGGATAAGCAGTTCTCAAG
CCACTCAACCTCTTGCCACGCCTGTAGTGTCTGTGACAACTCCGAGCTTGCCTCCACAGGGACTAGTGTA
CTCAGCAATGCCGACAGCCTACAACACTGATTACTCACTGACCAGCGCTGACCTGTCTGCCCTGCAAGGC
TTTACTTCCCCTGGAATGCTGTCTCTGGGGCAGGCTTCAGCCTGGCAGCAGCACCATCTAGGACAAGCAG
CCCTCAGCTCTCTGGTGGCTGGAGGGCAGTTATCTCAGGGTTCAAATTTGTCCATTAACACCAACCAGAA
CATCAACATTAAGTCAGAGCCAATTTCACCTCCCCGTGATAGAATGACCCCATCAGGCTTCCAACAGCAG
CAGCAGCAGCCACAACAGCAGCCACCGCCACAACCTCCACAGCCCCAACCCCGGCAGGAAATGGGGCGCT
CCCCTGTGGACAGTCTGAGCAGCTCCAGCAGCTCCTATGATGGCAGTGACCGGGAGGATCCACGGGGTGA
CTTCCATTCTCCAATTGTGCTTGGCCGACCTCCAAATACTGAGGACAGAGAAAGCCCTTCTGTAAAACGC
ATGAGGATGGACACATGGGTGACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207994 representing NM_001033713
Red=Cloning site Green=Tags(s)

MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSSNKLFQYASTDMDKVLLKYT
EYNEPHESRTNSDIVETLRKKGLNGCESPDADDYFEHSPLSEDRFSKLNEDSDFIFKRGPPGLPPQNFSM
SVTVPVTSPNALSYTNPGSSLVSPSLAASSTLADSSMLSPPPATLHRNVSPGAPQRPPSTGSASGMLSTT
DLTVPNGAGNSPVGNGFVNSRASPNLIGNTGANSLGKVMPTKSPPPPGGGSLGMNSRKPDLRVVIPPSSK
GMMPPLSEEEELELNAQRISSSQATQPLATPVVSVTTPSLPPQGLVYSAMPTAYNTDYSLTSADLSALQG
FTSPGMLSLGQASAWQQHHLGQAALSSLVAGGQLSQGSNLSINTNQNINIKSEPISPPRDRMTPSGFQQQ
QQQPQQQPPPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRESPSVKR
MRMDTWVT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033713
ORF Size 1494 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033713.2, NP_001028885.1
RefSeq Size 2898 bp
RefSeq ORF 1497 bp
Locus ID 17258
UniProt ID Q60929
Cytogenetics 7 36.72 cM
MW 54 kDa
Summary Transcriptional activator which binds specifically to the MEF2 element, 5'-YTAAT(4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001033713" in other vectors (6)
SKU Description Size Price
MC216964 Mef2a (untagged) - Mouse myocyte enhancer factor 2A (Mef2a), (10ug) 10 ug
$732.00
MG207994 Mef2a (tGFP-tagged) - Mouse myocyte enhancer factor 2A (Mef2a) 10 ug
$886.00
MR207994L1 Lenti ORF clone of Mef2a (Myc-DDK-tagged) - Mouse myocyte enhancer factor 2A (Mef2a) 10 ug
$986.00
MR207994L2 Lenti ORF clone of Mef2a (mGFP-tagged) - Mouse myocyte enhancer factor 2A (Mef2a) 10 ug
$986.00
MR207994L3 Lenti ORF clone of Mef2a (Myc-DDK-tagged) - Mouse myocyte enhancer factor 2A (Mef2a) 10 ug
$986.00
MR207994L4 Lenti ORF clone of Mef2a (mGFP-tagged) - Mouse myocyte enhancer factor 2A (Mef2a) 10 ug
$986.00

Plasmid Map for MR207994

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207994
Circular map for MR207994

file-alt Citations

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