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Fsd1 (NM_183178) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207971
Fsd1 (Myc-DDK-tagged) - Mouse fibronectin type 3 and SPRY domain-containing protein (Fsd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Fsd1 or NM_183178"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fsd1
Synonyms MGC58341
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207971 representing NM_183178
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGACCAGAGGGAGGCGCTAAGAAAAATCATCACTACCCTGGCCATGAAGAATGAGGAGACGCAGA
CCTTCATCTACTCCCTGAAGCAGATGTTGCTGAATGTAGAGGCAAACTCAGCCAAAGTCCAGGAGGATCT
GGAAGCCGAGTTCCAGTCCCTCACGTCGGTTCTGGAGGAGCTGAAAGAGTCCATGCTGATGAAGATCAAA
CAGGACAGGGCCAGCCGCACCTACGAGCTGCAGAACCAGCTAGCTGCCTGCACACGAGCCCTGGAGAGCT
CCGAGGAGCTCCTGGAAACAGCCAACCAGACCCTGCAGGCTTCAGACAGCGAGGACTTCAGTCAGGCCGC
CAAGGAAATCAAAGATGGCATAACTATGGCCCCTGCCTTCCGACTATCGCTCAAAGCCAAGGTGAGCGAC
AATATGAGTCACCTCATGGTGGACTTCGCCCAGGAAAGGCAGATGCTACAGGCCCTCAAGTTCCTACCTG
TGCCCAGCGCCCCCACCATCGACTTGGCAGAGTCCCTCGTGTCAGATAACTGTGTGACGCTGGTATGGCA
CATGCCAGATGAGGACAGCAAGATCGACCACTATGTGCTGGAGTACCGCAAGACCAATTTCGAGGGCCCA
CCCCGCCTCAAGGAGGACCACCCCTGGATGGTTGTCGAGGGCATCCGGCAGACCGAGCACACCTTGACAG
GTCTCAAATTTGACATGAAATACATGAACATTCGTGTGAAAGCCTGTAACAAGGCGGTGGCTGGTGAATT
CTCCGAGCCGGTGACCTTAGAGACACCAGCATTCATGTTCCGCCTGGACGGGTCCACATCTCACCAGAAC
CTGCGAGTGGAAGATCTCTCTGCCGAGTGGGACGCCATGGGCGGGAAGGTCCAAGATATCAAGGCTCGAG
AGAAAGAGGGCAAGGGACGGACCGCATCCCCTGTAAACTCTCCAGCCAGAGGGACTCCATCTCCCAAGAG
GATGTCCTCAGGTCGTGGGGGCCGGGACCGGTTCACAGCTGAGTCTTACACGGTGCTGGGGGACACACTG
ATTGATGGTGGCGAGCACTACTGGGAGGTGCGCTTCGAACCAGACAGCAAGGCCTTTGGCTTGGGTGTGG
CCTACCGCAGCCTGGGCCGATTCGAGCAGCTGGGCAAGACGGCTGCATCATGGTGCCTGCATGCCAACAA
CTGGCTGCAGGCCAGCTTCACCGCCAAGCACGCCAACAAGGTCAAAGTGCTGGACAGCCCTGTGCCTGAC
TGCCTGGGTGTGCACTGCGACTTCCACCAAGGCCTCCTATCTTTCTACAATGCCCGCACCAAACAGCTCC
TGCACACCTTCAAGGCCAAGTTCACGCAGCCTCTGCTTCCAGCCTTCACCGTATGGTGTGGAAGCTTCCA
GGTGACAACAGGCCTGCAGGTGCCCAGTGCCGTGCGCTGCCTGCAGAAGCGGGGCAGTGCCACTAGCAGT
TCCAATACCAGCCTCACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207971 representing NM_183178
Red=Cloning site Green=Tags(s)

MEDQREALRKIITTLAMKNEETQTFIYSLKQMLLNVEANSAKVQEDLEAEFQSLTSVLEELKESMLMKIK
QDRASRTYELQNQLAACTRALESSEELLETANQTLQASDSEDFSQAAKEIKDGITMAPAFRLSLKAKVSD
NMSHLMVDFAQERQMLQALKFLPVPSAPTIDLAESLVSDNCVTLVWHMPDEDSKIDHYVLEYRKTNFEGP
PRLKEDHPWMVVEGIRQTEHTLTGLKFDMKYMNIRVKACNKAVAGEFSEPVTLETPAFMFRLDGSTSHQN
LRVEDLSAEWDAMGGKVQDIKAREKEGKGRTASPVNSPARGTPSPKRMSSGRGGRDRFTAESYTVLGDTL
IDGGEHYWEVRFEPDSKAFGLGVAYRSLGRFEQLGKTAASWCLHANNWLQASFTAKHANKVKVLDSPVPD
CLGVHCDFHQGLLSFYNARTKQLLHTFKAKFTQPLLPAFTVWCGSFQVTTGLQVPSAVRCLQKRGSATSS
SNTSLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183178
ORF Size 1488 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183178.2, NP_899001.1
RefSeq Size 1748 bp
RefSeq ORF 1491 bp
Locus ID 240121
UniProt ID Q7TPM6
Cytogenetics 17 D
MW 55.5 kDa
Summary May be involved in microtubule organization and stabilization.UniProtKB/Swiss-Prot Function
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Documents
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"NM_183178" in other vectors (4)
SKU Description Size Price
MC205121 Fsd1 (untagged) - Mouse fibronectin type 3 and SPRY domain-containing protein (Fsd1), (10ug) 10 ug
$457.00
MG207971 Fsd1 (tGFP-tagged) - Mouse fibronectin type 3 and SPRY domain-containing protein (Fsd1) 10 ug
$657.00
MR207971L3 Lenti ORF clone of Fsd1 (Myc-DDK-tagged) - Mouse fibronectin type 3 and SPRY domain-containing protein (Fsd1) 10 ug
$757.00
MR207971L4 Lenti ORF clone of Fsd1 (mGFP-tagged) - Mouse fibronectin type 3 and SPRY domain-containing protein (Fsd1) 10 ug
$757.00

Plasmid Map for MR207971

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207971
Circular map for MR207971

file-alt Citations

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