Fkrp (NM_173430) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207921
Fkrp (Myc-DDK-tagged) - Mouse fukutin related protein (Fkrp)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fkrp
Synonyms A830029B19Rik; AI842067; AI847300; LGMD1I; MDC1C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207921 representing NM_173430
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCTCACCCGCTGCTGGGCTGCCCTGGCAGCCGCCATCATCCTCAACCTCCTAGTCTTCTTCTATG
TGTCATGGCTACAACACCAGCCCAGAAACTCCCGGGCCCGGGGTCCCCGCCGGACTTCTGCCATTGGCCC
CCGAGTCACCGTCCTGATTCGGGAATTTGAGGCTTTTGACAACGCGGTGCCAGAGCTAGTGGATTCCTTT
TTGCAGCAGGACCCAGCCCAGCCCGTGGTAGTGGCGGCCGACACACTCCCTTACCCACCCCTGGCCTTGC
CTCGCATCCCCAACGTTCGCCTGGCTCTGCTCCAGCCAGCCCTGGACCGGCCAGCGGCGGCCTCGCGCCC
GGAGACCTACGTAGCCACCGAGTTTGTGGCTCTAGTGCCTGATGGAGCGCGGGCCGAGTCACCAGGCCAC
CTGGAGCGAATGGTGGAGGCGCTCCGAGGGAGCAGCGCGCGCCTAGTGGCCGCCCCGGTCGCCACCGCCA
ACCCAGCGCGGTGCCTAGCTCTGAACGTCAGCCTGCGGGAGTGGACTGCGCGCTACGACCCAGCTCCCAG
CGCGCCCCGCTGCGACGCTTTGGATGGCGACGCTGTGCTGCTGATGCGCTCCCGCGACCTCTTCAACCTC
TCGGTGCCCCTGGCGCGGCCGCTGGCCACCAGCCTCTTCCTACAGACCGCCCTGCGCGGCTGGGCAGTGC
AGCTGCTGGACTTGACCTTCGCCGCGGCGCGCCAGCCACCGCTGGCCACCGCCCACGCGCGCTGGAAGGC
GGAACGTGAGGGGCGCTCACGGAGAGCGGCGCTGCTGCGCTCGTTGGGAATCCGTCTGGTGAGCTGGGAA
GGCGGGCGGCTAGAGTGGTTTGGCTGCAGCAAGGAGAGCGCGCGCTGCTTCGGTACGGTGGCGGGCGACA
CACCCGCCTACCTGTATGAGGGCCGCTGGACCCCACCTTGTTGCCTGCGCGCGCTGCGCGAGACTGCGCG
CTACGTGGTGGGCGTGCTGGAGGCGGCGGGCGTGCGCTACTGGTTGGAGGGCGGCTCGCTGCTGGGTGCA
GCTCGCCACGGCGACATCATCCCTTGGGACTACGACGTAGATCTGGGCATCTACCTGGAGGACGTGGGCA
ACTGCGAGCAGTTGCGGGGTGCCGAAGCTGGCTCGGTAGTGGATGAACGCGGCTTTGTGTGGGAGAAGGC
GGTGGAGGGCGACTTCTTCCGAGTACAGTACAGTGAGAACAACCACCTGCACGTGGACCTGTGGCCCTTT
TACCCCCGCAATGGGGTTATGACCAAGGACACGTGGCTGGACCACCGGCAGGATGTTGAGTTCCCAGAGC
ACTTCCTGCAGCCACTTGTCCCCCTGCCCTTTGCGGGTTTCATGGCACAGGCCCCTAACAACTACCGCCG
CTTCCTGGAGCTGAAGTTTGGGCCTGGGGTCATCGAGAACCCGGAGTACCCCAACCCCGCACTCTTAAGC
TTGACAGGCGGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207921 representing NM_173430
Red=Cloning site Green=Tags(s)

MRLTRCWAALAAAIILNLLVFFYVSWLQHQPRNSRARGPRRTSAIGPRVTVLIREFEAFDNAVPELVDSF
LQQDPAQPVVVAADTLPYPPLALPRIPNVRLALLQPALDRPAAASRPETYVATEFVALVPDGARAESPGH
LERMVEALRGSSARLVAAPVATANPARCLALNVSLREWTARYDPAPSAPRCDALDGDAVLLMRSRDLFNL
SVPLARPLATSLFLQTALRGWAVQLLDLTFAAARQPPLATAHARWKAEREGRSRRAALLRSLGIRLVSWE
GGRLEWFGCSKESARCFGTVAGDTPAYLYEGRWTPPCCLRALRETARYVVGVLEAAGVRYWLEGGSLLGA
ARHGDIIPWDYDVDLGIYLEDVGNCEQLRGAEAGSVVDERGFVWEKAVEGDFFRVQYSENNHLHVDLWPF
YPRNGVMTKDTWLDHRQDVEFPEHFLQPLVPLPFAGFMAQAPNNYRRFLELKFGPGVIENPEYPNPALLS
LTGG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173430
ORF Size 1482 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173430.2, NP_775606.1
RefSeq Size 2817 bp
RefSeq ORF 1485 bp
Locus ID 243853
UniProt ID Q8CG64
Cytogenetics 7 A2
MW 55.3 kDa
Summary Catalyzes the transfer of CDP-ribitol to ribitol 5-phosphate previously attached by FKTN/fukutin of to the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (By similarity). This constitutes the second step in the formation of the ribose 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_173430" in other vectors (4)
SKU Description Size Price
MC216904 Fkrp (untagged) - Mouse fukutin related protein (Fkrp), (10ug) 10 ug
$457.00
MG207921 Fkrp (tGFP-tagged) - Mouse fukutin related protein (Fkrp) 10 ug
$657.00
MR207921L3 Lenti ORF clone of Fkrp (Myc-DDK-tagged) - Mouse fukutin related protein (Fkrp) 10 ug
$757.00
MR207921L4 Lenti ORF clone of Fkrp (mGFP-tagged) - Mouse fukutin related protein (Fkrp) 10 ug
$757.00

Plasmid Map for MR207921

Download sequence for

Cloning scheme for MR207921

Circular map for MR207921

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