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Pigv (NM_178698) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207911
Pigv (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class V (Pigv), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Pigv or NM_178698"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pigv
Synonyms B330013B03; D430024F16Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207911 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACTCCTGGACCCATCCCAGAAAGAGGTGCTGAGATTCGCAGTAAACTGCCGCATCCTGACTCTAG
TGCTACAGGCACTCTTCAACCTCATCATCCCAGATCACCACGCAGATGCCTTTTGCCCTCCCCGCCTCGC
CCCCTCGGGCTCTGCGGATCAGCTTGTGGAAGGTCTTCTGGGTGGTCTGTCTCGATGGGATGCTGAACAC
TTCCTGTTTATCGCTGAGCACGGCTACCTTTATGAGCACAACTTTGCCTTCTTCCCTGGCTTTCCCTTGG
CCCTGCTGATGGGGACTGAGCTGCTGAGACCCTTGCAGGGCCTCTTGAGCCAGCGCAGTTGCCTGCTGGT
CTCAGTAGCACTTCTCAACTTGCTGTTCTCCGTGCTGGCTGCAGTGGCACTCCATGACTTGGGTTGTCTG
GTTCTGCACTGTCCTCGGCAGGCCTTGTGTGCAGCGCTGCTCTTCTGCATCAGTCCTGCCAACGTTTTCC
TGGCAGCCGGCTACTCAGAGGCTTTGTTTGCCTTCCTGACGTTCAGCGCCATGGGGCAGCTGGAGAGGGG
CCGTGGCTGGGCTAGTGGGCTGCTCTTTGCTCTTGCTGCTGGGGTGCGCTCCAATGGGCTGGTGAGCCTT
GGCTTCCTCCTGCACTCCCAGTGCAGAGGCTTTTGTTCCTCTCTGGCGGTGCTGAGCCCCTGGAAGCCCC
TCGTGAAGCTGATGGCCTCTGTGTGCTTGTCGGTGCTCATAGTCAGCCTTCCCTTCGCCCTCTTTCAGTA
TCGTGCCTACATCCAGTTCTGCTCACCAGGCTCAGCCCCCTCCATCCCTGAGCCCTTGCTGCAGCTAGCT
GCCGACAAAGGCTACCGGCTTGCAGGAGAAAATGCACCCCCATGGTGCTCCTGGGACCTTCCTCTAATAT
ACAACTATATTCAGGATGTCTACTGGAATGTTGGTCTTCTGCGATACTATGAGCTCAAACAGGTGCCCAA
TTTCCTACTAGCTACACCAGTGACAGTACTGGTTGTGTGGGCAACCTGGACATATGTGACCACCCACCCT
TGGCTCTGCCTTACACTTGGGCTGCAAAGAACCAAGGACAGGGAGAACCCGGAGAAGCCCCACCGTGGCT
TCCTGAGTCCGAAGGTGTTTGTGTACCTGGTCCACGCTGCAGCACTGCTGGTCTTTGGAGGTCTGTGCAT
GCATGTCCAGGTTCTCACGCGGTTTTTGGCCTCTTCCACTCCTATCATGTACTGGTTTCCAGCTCACCTG
CTTCAGGATCAAGAACCGCTGCTGAGGTGTGTGGACACAGAGCCTGGGAAGCTTCCTCAGGAGAAGTCCC
CACCAGGACAGAAGGCCCCCAGAAACTGTCTCATGAAACTCTTCTACAATTGGAAGAGATGCTCTCCAGT
CACAAGATGCGTTCTGGTCTACTTCCTGACCTACTGGCTTCTGGGACTAATTCTCCATTGCAACTTCCTG
CCTTGGACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207911 protein sequence
Red=Cloning site Green=Tags(s)

MGLLDPSQKEVLRFAVNCRILTLVLQALFNLIIPDHHADAFCPPRLAPSGSADQLVEGLLGGLSRWDAEH
FLFIAEHGYLYEHNFAFFPGFPLALLMGTELLRPLQGLLSQRSCLLVSVALLNLLFSVLAAVALHDLGCL
VLHCPRQALCAALLFCISPANVFLAAGYSEALFAFLTFSAMGQLERGRGWASGLLFALAAGVRSNGLVSL
GFLLHSQCRGFCSSLAVLSPWKPLVKLMASVCLSVLIVSLPFALFQYRAYIQFCSPGSAPSIPEPLLQLA
ADKGYRLAGENAPPWCSWDLPLIYNYIQDVYWNVGLLRYYELKQVPNFLLATPVTVLVVWATWTYVTTHP
WLCLTLGLQRTKDRENPEKPHRGFLSPKVFVYLVHAAALLVFGGLCMHVQVLTRFLASSTPIMYWFPAHL
LQDQEPLLRCVDTEPGKLPQEKSPPGQKAPRNCLMKLFYNWKRCSPVTRCVLVYFLTYWLLGLILHCNFL
PWT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178698
ORF Size 1479 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178698.1, NM_178698.2, NM_178698.3, NM_178698.4, NM_178698.5, NP_848813.2
RefSeq Size 2941 bp
RefSeq ORF 1482 bp
Locus ID 230801
UniProt ID Q7TPN3
Cytogenetics 4 D2.3
MW 55 kDa
Summary Alpha-1,6-mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178698" in other vectors (4)
SKU Description Size Price
MC205891 Pigv (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class V (Pigv), transcript variant 1, (10ug) 10 ug
$457.00
MG207911 Pigv (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class V (Pigv) 10 ug
$657.00
MR207911L3 Lenti ORF clone of Pigv (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class V (Pigv), transcript variant 1 10 ug
$757.00
MR207911L4 Lenti ORF clone of Pigv (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class V (Pigv), transcript variant 1 10 ug
$757.00

Plasmid Map for MR207911

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207911
Circular map for MR207911

file-alt Citations

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