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Slc16a1 (NM_009196) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207904
Slc16a1 (Myc-DDK-tagged) - Mouse solute carrier family 16 (monocarboxylic acid transporters), member 1 (Slc16a1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc16a1
Synonyms AL022710; Mct1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207904 representing NM_009196
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCACCTGCGATTGGAGGGCCAGTGGGATACACCCCCCCAGATGGAGGCTGGGGGTGGGCAGTGTTAG
TCGGAGCCTTCATTTCTATTGGCTTCTCCTATGCATTTCCCAAATCCATCACTGTCTTCTTTAAAGAGAT
AGAAGTTATATTCAGTGCAACGACCAGTGAAGTATCATGGATATCATCTATAATGTTGGCTGTCATGTAT
GCTGGAGGTCCTATCAGCAGTATCTTGGTGAATAAATACGGCAGCCGTCCAGTAATGATCGCTGGTGGTT
GTCTGTCTGGTTGCGGCTTGATCGCAGCTTCTTTCTGTAACACAGTACAGGAACTTTACTTGTGCATTGG
TGTTATTGGAGGTCTTGGGCTTGCTTTCAACTTGAACCCAGCTCTGACTATGATTGGCAAGTATTTCTAC
AAGAAGCGACCACTGGCCAACGGACTGGCCATGGCAGGCAGCCCTGTGTTCCTCTCTACCCTGGCTCCAC
TTAATCAGGCTTTCTTTGATATTTTTGACTGGAGAGGAAGCTTCCTAATTCTTGGGGGCCTCCTCCTAAA
TTGTTGTGTAGCTGGATCCCTGATGAGACCAATAGGGCCTGAGCAAGTCAAGCTAGAAAAACTCAAGTCC
AAAGAATCTCTACAGGAAGCTGGAAAATCTGATGCAAATACAGATCTCATTGGAGGAAGTCCCAAAGGAG
AAAAGCTGTCCGTCTTCCAAACAATTAACAAATTCCTGGACTTGTCGCTGTTTACCCATAGGGGCTTTTT
ACTGTACCTGTCTGGAAATGTGGTCATGTTTTTTGGACTCTTTACCCCTTTGGTCTTTCTTAGTAGTTAT
GGTAAGAGTAAGGATTTTTCCAGTGAGAAATCAGCCTTCCTTCTTTCCATTTTGGCTTTTGTTGATATGG
TAGCCAGACCGTCCATGGGACTTGCAGCCAACACCAAGTGGATCAGACCTCGGATCCAGTACTTTTTTGC
TGCTTCTGTTGTTGCAAATGGAGTGTGCCATTTGCTTGCCCCTTTGTCTACAACCTACGTTGGGTTCTGT
GTCTACGCCGGAGTCTTTGGATTTGCCTTTGGTTGGCTCAGCTCTGTATTATTTGAAACATTGATGGACC
TCATTGGACCCCAGAGGTTCTCCAGTGCTGTGGGCTTGGTGACCATTGTGGAATGCTGCCCTGTCCTCCT
AGGGCCACCACTTTTAGGCCGCCTCAATGACATGTATGGAGACTACAAATACACGTACTGGGCTTGTGGC
GTGATCCTCATCATCGCGGGTATCTATCTCTTCATTGGCATGGGCATCAACTATCGACTTCTTGCCAAAG
AACAGAAAGCGGAGGAGAAGCAGAAAAGGGAAGGAAAAGAGGACGAGGCCAGCACCGATGTCGACGAGAA
GCCAAAGGAGACGATGAAAGCTGCACAGTCGCCGCAGCAGCACAGCTCCGGGGACCCCACAGAGGAGGAG
AGCCCTGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207904 representing NM_009196
Red=Cloning site Green=Tags(s)

MPPAIGGPVGYTPPDGGWGWAVLVGAFISIGFSYAFPKSITVFFKEIEVIFSATTSEVSWISSIMLAVMY
AGGPISSILVNKYGSRPVMIAGGCLSGCGLIAASFCNTVQELYLCIGVIGGLGLAFNLNPALTMIGKYFY
KKRPLANGLAMAGSPVFLSTLAPLNQAFFDIFDWRGSFLILGGLLLNCCVAGSLMRPIGPEQVKLEKLKS
KESLQEAGKSDANTDLIGGSPKGEKLSVFQTINKFLDLSLFTHRGFLLYLSGNVVMFFGLFTPLVFLSSY
GKSKDFSSEKSAFLLSILAFVDMVARPSMGLAANTKWIRPRIQYFFAASVVANGVCHLLAPLSTTYVGFC
VYAGVFGFAFGWLSSVLFETLMDLIGPQRFSSAVGLVTIVECCPVLLGPPLLGRLNDMYGDYKYTYWACG
VILIIAGIYLFIGMGINYRLLAKEQKAEEKQKREGKEDEASTDVDEKPKETMKAAQSPQQHSSGDPTEEE
SPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009196
ORF Size 1479 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009196.4
RefSeq Size 4428 bp
RefSeq ORF 1482 bp
Locus ID 20501
UniProt ID P53986
Cytogenetics 3 F2.2
MW 53.7 kDa
Summary Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009196" in other vectors (4)
SKU Description Size Price
MC203709 Slc16a1 (untagged) - Mouse solute carrier family 16 (monocarboxylic acid transporters), member 1 (Slc16a1), (10ug) 10 ug
$457.00
MG207904 Slc16a1 (tGFP-tagged) - Mouse solute carrier family 16 (monocarboxylic acid transporters), member 1 (Slc16a1) 10 ug
$489.00 $657.00
MR207904L3 Lenti ORF clone of Slc16a1 (Myc-DDK-tagged) - Mouse solute carrier family 16 (monocarboxylic acid transporters), member 1 (Slc16a1) 10 ug
$757.00
MR207904L4 Lenti ORF clone of Slc16a1 (mGFP-tagged) - Mouse solute carrier family 16 (monocarboxylic acid transporters), member 1 (Slc16a1) 10 ug
$757.00

Plasmid Map for MR207904

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207904
Circular map for MR207904

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