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Zfp639 (NM_144519) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207772
Zfp639 (Myc-DDK-tagged) - Mouse zinc finger protein 639 (Zfp639), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zfp639
Synonyms 6230400O18Rik; ANC-2H01; ZASC1; Znf639
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207772 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGAATATCCTAAAAAAAGAAAAAGGAAGACTTTACACCCTTCTCGCTATTCAGATTCTTCTGGAA
TAAGCAGAATTGCAGATGGAGTCAGTGGGATTTTTTCTGATCACTGTTACAGTGTCTGCTCTATGAGACA
GCCAGACTTAAAATATTTTGACAACAAAGATGATGATTCTGATCCTGAGACAGCAAATGACTTGCCCAAA
TTTGCAGATGGAACCAAGGCCAGAAACAGGAATCAGAGCTACCTGGTTCCCAGCCCTGTGCTTAGGATTC
TAGACCACACTGTCTTTTCCACAGAAAAATCTACTGAAGTTGAGATCTGTGATGAAGAATGTGCCTCACC
TGAGTCAGTGCATCAGCATACTCAAGAGGAGAGCCCTATAGAAGTTCATACCTCAGAAGACGTCCCAATT
GCTGTAGAAGTTCATGCAATCTCTGAAGACTATGATATAGAGGCAGAGAACAATTCCTCTGAGAGCCTCC
AAGACCAGGCCGATGAAGAACCACCAGCTAAACTCTGTAAAATTCTTGACAAGGGCCAGGCTTTGAATGT
GACTGCCCAACAGAAGTGGCCTCTCCTGAGAGCCAACAGCAGTGGTCTCTACAAGTGTGAACTTTGTGAA
TTTAACAGTAAATACTTTTCTGACCTAAAGCAACATGTGATCCTGAAACACAAGCGCACTGACTCGAATG
TGTGTCGGGTGTGTAAGGAAAGCTTCTCTACCAACATGCTTCTCATTGAACACGCCAAACTCCATGAAGA
AGACCCCTACATCTGTAAGTACTGTGATTACAAGACTGTGATCTTTGAGAACCTCAGCCAGCACATTGCA
GACACGCACTTCAGCGACCACCTTTACTGGTGTGAGCAGTGTGACGTGCAGTTCTCCTCAAGCAGTGAGC
TCTACCTGCACTTCCAGGAGCATAGCCGTGATGAGCAGTACCTGTGCCAGTTCTGTGAGCACGAGACAGG
GGACCCTGAGGACTTGCACAGCCACGTTGTCAACGAGCACGCTCGCAGACTGATTGAGCTGAGTGACAAG
TGTGGCAGTGGTGGCCATGGGCAGTGCAGCCTTTTAAGCAAGATCACCTTTGACAAATGTAAAAATTTCT
TCGTGTGTCAAGTATGTGGCTTTCGGAGCAGACTTCACACAAATGTCAACAGACACGTGGCTATCGAGCA
TACTAAAATATTCCCTCATGTTTGTGATGACTGTGGGAAGGGCTTTTCCAGCATGTTGGAATATTGCAAG
CATCTAAATTCACATCTATCTGAAGGGATTTACTTATGCCAATATTGTGAATATTCAACAGGACAAATTG
ACGATCTTAAAATTCATCTAGATTTCAAGCATTCAGCTGACTTGCCTCATAAATGTAGTGAGTGCTTGAT
GAGGTTTGGGAACGAAAGGGACTTACTCGGTCACCTTCAAGTCCATGAGACTACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207772 protein sequence
Red=Cloning site Green=Tags(s)

MNEYPKKRKRKTLHPSRYSDSSGISRIADGVSGIFSDHCYSVCSMRQPDLKYFDNKDDDSDPETANDLPK
FADGTKARNRNQSYLVPSPVLRILDHTVFSTEKSTEVEICDEECASPESVHQHTQEESPIEVHTSEDVPI
AVEVHAISEDYDIEAENNSSESLQDQADEEPPAKLCKILDKGQALNVTAQQKWPLLRANSSGLYKCELCE
FNSKYFSDLKQHVILKHKRTDSNVCRVCKESFSTNMLLIEHAKLHEEDPYICKYCDYKTVIFENLSQHIA
DTHFSDHLYWCEQCDVQFSSSSELYLHFQEHSRDEQYLCQFCEHETGDPEDLHSHVVNEHARRLIELSDK
CGSGGHGQCSLLSKITFDKCKNFFVCQVCGFRSRLHTNVNRHVAIEHTKIFPHVCDDCGKGFSSMLEYCK
HLNSHLSEGIYLCQYCEYSTGQIDDLKIHLDFKHSADLPHKCSECLMRFGNERDLLGHLQVHETT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144519
ORF Size 1455 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144519.4, NP_653102.1
RefSeq Size 2038 bp
RefSeq ORF 1458 bp
Locus ID 67778
UniProt ID Q99KZ6
Cytogenetics 3 A3
MW 55.7 kDa
Summary Binds DNA and may function as a transcriptional repressor.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144519" in other vectors (4)
SKU Description Size Price
MC216600 Zfp639 (untagged) - Mouse zinc finger protein 639 (Zfp639), transcript variant 1, (10ug) 10 ug
$503.00
MG207772 Zfp639 (tGFP-tagged) - Mouse zinc finger protein 639 (Zfp639) 10 ug
$657.00
MR207772L3 Lenti ORF clone of Zfp639 (Myc-DDK-tagged) - Mouse zinc finger protein 639 (Zfp639), transcript variant 1 10 ug
$757.00
MR207772L4 Lenti ORF clone of Zfp639 (mGFP-tagged) - Mouse zinc finger protein 639 (Zfp639), transcript variant 1 10 ug
$757.00

Plasmid Map for MR207772

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207772
Circular map for MR207772

file-alt Citations

ZBTB2 reads unmethylated CpG island promoters and regulates embryonic stem cell differentiation
Karemaker, Vermeulen
EMBO Rep (2018) 19 (4) DOI: 10.15252/embr.201744993
Cited by 33
AI Summary: In this study, OriGene’s ZBTB2 expression plasmid (Cat# MR207779) and ZBTB2 shRNA plasmids (Cat# TL514060) were used to manipulate ZBTB2 levels in mouse embryonic stem cells, enabling the authors to investigate ZBTB2’s role as a reader of unmethylated CpG island promoters and its impact on stem cell differentiation.
Showing 1-1 of 1 papers.
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