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Cdc14b (NM_172587) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207764
Cdc14b (Myc-DDK-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdc14b
Synonyms 2810432N10Rik; A530086E13Rik; AA472821; Cdc14B1; CDC14B3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207764 representing NM_172587
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCGTAAGAGCGAGCGGCGCTCGGCTTGGGCCACCGCGCCCCCCTGCTCACGCCGCAGCTCGTCCT
CCTCTCCCGGCGTGAAGAAGAGCCGCAGCTCCACGCCGCAGGAGCTGCACCGCCTGGAGCAGCAGGACGA
CCTGTACCTGGACATCACGGATCGCCTTTGTTTTGCCATTCTCTACAGCAGACCAAAGAGTGCAACAAAC
GAACATTATTTCAGCATAGATAACGAGCTAGAGTATGAGAACTTCTATGCGGATTTTGGACCACTCAATC
TGGCCATGGTTTACAGATATTGTTGCAAGATAAATAAGAAGCTCAAGTCCATTACAATGCTGAGGAAGAA
AATTATCCATTTTACTGGCACTGATCAGAGAAAGCAAGCGAATGCCGCCTTCCTTGTCGGGTGTTATATG
GTTATATATTTGGGGAGGACACCAGAAGACGCGTATAGAACATTAATCTTCGGAGATACAGCCTATATTC
CTTTCAGAGATGCTGCCTATGGAAGCTGCAGTTTCTATATTACACTTCTCGACTGTTTTCACGCAGTAAA
GAAGGCAATGCAGTATGGCTTCTTTAATTTCAACTCATTTAACCTTGACGAATATGAACACTATGAAAAA
GCAGAAAATGGAGATTTCAACTGGATAATACCAGAGCGATTTCTTGCCTTCTGTGGGCCCCATTCAAGAT
CCAGGCTGGAAAGTGGTTACCACCAACATTCTCCGGAGACTTATATTCCATATTTTAAGAATCACAATGT
AACTACCATTATTCGTCTGAATAAAAGGATGTATGATGCTAAGCGCTTTACGGATGCTGGATTCGATCAC
CATGATCTTTTCTTTCCGGATGGCAGCACCCCTGCTGAGTCAATTGTCCAAGAATTTCTGGATATTTGTG
AAAATGTCAAGGGTGCCATTGCTGTGCATTGCAAAGCTGGCCTTGGTCGAACAGGCACTCTGATAGGCTG
CTACCTCATGAAGCATTACAGGATGACAGCAGCTGAGAGCATTGCCTGGCTCAGAATCTGTAGACCCGGC
TCAGTAATTGGGCCCCAGCAACAGTTTCTGGTCATGAAACAATCAAGCCTCTGGCTAGAAGGCGACTATT
TCCGTCAAAAGTTAAGAGGGCAGGAGAATGGCCCCCTCAGAGAAGCCTTCTCCAAACACCTTTCGGATGC
TGATGACCTGTCCTTAAATGGGCTTGAGAATCAAGACAATCAAGAGCCTGAGCCGTATAGTGATGATGAT
GAAGTCAGTGGAATGACACAAGGAGACAGACTTCGGGCCCTGAAAAGCCGGAGACAGCCGAAAGCCAGCG
CTATCCCCCTCACATGTCCCCTAGCTGTGCTGACCTCTGCACTGTGTAGTGTTGCCATCTGGTGGATTGT
GTGTGACTACATTCTTCCCACCCTGCTGTTCTGTCTTGATGGCTTCAGAACACAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207764 representing NM_172587
Red=Cloning site Green=Tags(s)

MKRKSERRSAWATAPPCSRRSSSSSPGVKKSRSSTPQELHRLEQQDDLYLDITDRLCFAILYSRPKSATN
EHYFSIDNELEYENFYADFGPLNLAMVYRYCCKINKKLKSITMLRKKIIHFTGTDQRKQANAAFLVGCYM
VIYLGRTPEDAYRTLIFGDTAYIPFRDAAYGSCSFYITLLDCFHAVKKAMQYGFFNFNSFNLDEYEHYEK
AENGDFNWIIPERFLAFCGPHSRSRLESGYHQHSPETYIPYFKNHNVTTIIRLNKRMYDAKRFTDAGFDH
HDLFFPDGSTPAESIVQEFLDICENVKGAIAVHCKAGLGRTGTLIGCYLMKHYRMTAAESIAWLRICRPG
SVIGPQQQFLVMKQSSLWLEGDYFRQKLRGQENGPLREAFSKHLSDADDLSLNGLENQDNQEPEPYSDDD
EVSGMTQGDRLRALKSRRQPKASAIPLTCPLAVLTSALCSVAIWWIVCDYILPTLLFCLDGFRTQ

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_172587
ORF Size 1455 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172587.3, NP_766175.3
RefSeq Size 5859 bp
RefSeq ORF 1458 bp
Locus ID 218294
UniProt ID Q6PFY9
Cytogenetics 13 B3
MW 55.7 kDa
Summary Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172587" in other vectors (4)
SKU Description Size Price
MC216713 Cdc14b (untagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 1, (10ug) 10 ug
$503.00
MG207764 Cdc14b (tGFP-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b) 10 ug
$657.00
MR207764L3 Lenti ORF clone of Cdc14b (Myc-DDK-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 1 10 ug
$757.00
MR207764L4 Lenti ORF clone of Cdc14b (mGFP-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 1 10 ug
$757.00

Plasmid Map for MR207764

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207764
Circular map for MR207764

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