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Pde7a (NM_001122759) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207723
Pde7a (Myc-DDK-tagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 2
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pde7a
Synonyms AU015378; AW047537
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207723 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGTATGCTACCAGCTGCCGGTGCTGCCCCTGGACAGGCCGGTCCCCCAGCACGTCCTCAGCCGCC
GAGGAGCCATCAGCTTCAGCTCCAGCTCCGCGCTCTTCGGCTGCCCGCATCCCCGGCAGCTCTCGCAGAG
ACGTGGAGCTATTTCCTATGACAGTTCTGATCAGACTGCGTTATATATTCGTATGCTAGGAGATGTCCGC
GTGAGGAGCCGAGCAGGATTTGAAACAGAAAGAAGAGGTTCCCATCCGTACATTGACTTCCGTATTTTTC
ACTCTCAATCTGACATTGAAGCATCAGTCTCCGCCAGGAACATCAGAAGGTTACTAAGTTTCCAGCGATA
TCTCAGATCCTCACGAGTTTTTCGGGGTGCCACAGTTTGTAGTTCTCTAGACATTTTAGATGAGGATTAC
AATGGACAAGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATATCTTTCTGTTTGATAGAC
TAACAAATGGAAATAGTCTAGTAAGCCTAACCTTTCATTTATTTAGTCTTCATGGATTGATTGAGTACTT
CCATTTAGATATGGTGAAACTCCGTCGGTTTTTAGTTATGATTCAAGAAGATTACCACAGTCAAAATCCT
TACCACAATGCAGTCCATGCTGCAGACGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTG
CCAGTTCTGTCACTCCTTGGGATATCTTGCTGAGTTTAATTGCAGCCGCCACTCACGATCTGGATCACCC
AGGTGTTAATCAGCCGTTTCTTATTAAAACCAACCATTATCTAGCAACTTTATACAAGAATTCCTCAGTC
CTGGAGAATCACCACTGGAGATCTGCAGTGGGCTTGTTAAGAGAATCTGGTTTGTTCTCACACTTGCCAT
TGGAAAGCAGGCAAGAGATGGAGGCTCAGATAGGTGCTTTGATATTAGCCACGGATATCAGTCGCCAGAA
CGAGTACCTGTCATTGTTTAGATCTCACTTGGATAAAGGTGACTTACACCTTGACGATGGCGGACATAGG
CATTTGGTTCTACAGATGGCCTTGAAATGTGCTGATATTTGTAACCCATGTCGGAACTGGGAATTAAGCA
AGCAGTGGAGTGAAAAAGTAACGGAGGAATTCTTCCACCAAGGAGATATAGAAAAAAAGTACCATTTGGG
TGTGAGTCCACTTTGTGATCGTCAGACTGAGTCTATTGCCAACATCCAGATTGGTTTTATGACTTACCTA
GTGGAGCCTTTATTTACAGAGTGGGCCAGGTTTTCAGACACACGGCTGTCACAGACGATGCTTGGACATG
TGGGGCTGAATAAAGCCAGCTGGAAGGGACTGCAGAGACAACAGCCCAGCAGCGAGGATGCCAATGCTGC
ATTTGAGTTGAACTCACAGTTACTAACTCAGGAAAATCGGTTATCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207723 protein sequence
Red=Cloning site Green=Tags(s)

MEVCYQLPVLPLDRPVPQHVLSRRGAISFSSSSALFGCPHPRQLSQRRGAISYDSSDQTALYIRMLGDVR
VRSRAGFETERRGSHPYIDFRIFHSQSDIEASVSARNIRRLLSFQRYLRSSRVFRGATVCSSLDILDEDY
NGQAKCMLEKVGNWNFDIFLFDRLTNGNSLVSLTFHLFSLHGLIEYFHLDMVKLRRFLVMIQEDYHSQNP
YHNAVHAADVTQAMHCYLKEPKLASSVTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYKNSSV
LENHHWRSAVGLLRESGLFSHLPLESRQEMEAQIGALILATDISRQNEYLSLFRSHLDKGDLHLDDGGHR
HLVLQMALKCADICNPCRNWELSKQWSEKVTEEFFHQGDIEKKYHLGVSPLCDRQTESIANIQIGFMTYL
VEPLFTEWARFSDTRLSQTMLGHVGLNKASWKGLQRQQPSSEDANAAFELNSQLLTQENRLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001122759
ORF Size 1446 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001122759.1, NM_001122759.2, NP_001116231.1
RefSeq Size 6298 bp
RefSeq ORF 1449 bp
Locus ID 18583
UniProt ID P70453
Cytogenetics 3 5.36 cM
MW 55.2 kDa
Summary Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_001122759" in other vectors (3)
SKU Description Size Price
MC216639 Pde7a (untagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 2, (10ug) 10 ug
$503.00
MR207723L3 Lenti ORF clone of Pde7a (Myc-DDK-tagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 2 10 ug
$757.00
MR207723L4 Lenti ORF clone of Pde7a (mGFP-tagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 2 10 ug
$757.00

Plasmid Map for MR207723

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207723
Circular map for MR207723

file-alt Citations

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