Agfg2 (NM_178162) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207679
Agfg2 (Myc-DDK-tagged) - Mouse ArfGAP with FG repeats 2 (Agfg2), transcript variant 2
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Agfg2
Synonyms A630095P14Rik; Hr; Hrbl; RAB-R; RABR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207679 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGATGGCGGCTAAGAAGGGCCCGGGCCCGGGTGGCGGGGTCGGAGGGAGCAAAGCGGAGGCTGAAG
CCGCTTCGGAGGTGTGGTGCCGCCGAGTGCGGGAGCTGGGCGGCTGCAGCCAGGCCGGGAACCGCCACTG
TTTCGAGTGCGCCCAGCGCGGGGTCACGTATGTGGACATCACCGTGGGCAGCTTCGTCTGCACCACCTGC
TCCGGCCTCCTGAGAGGCCTGAACCCCCCTCATCGAGTCAAGTCAATCTCCATGACAACTTTCACTGAGC
CTGAAGTCCTGTTCCTCCAATCTCGTGGAAATGAGGTCTGTCGGAAAATCTGGCTCGGTCTTTTTGATGC
TCGGACATCGTTGATACCAGATTCCAGGGATCCTCAGAAGGTGAAGGAGTTTCTCCAAGAAAAATATGAG
AAAAAAAGATGGTACGTCCCCCCAGAGCAAGTCAAGGGGCCCTCTTACAGCAAAGGCAGTGTCTCTGCTA
CCCCTGTCCAGGGCTCTGTCCCAGAAGGGAAACCCATTCGGACACTTCTGGGAGACCCTGTGCCATCTCT
CTCTGATCCTGCTTCCACTTCAAGCCAGCCTGGGAGCCAGTCGCAGGCACGCAGCAGCTCGCAGGCCAGG
AGCTCCCAGCCTCCTTCCCATTCATCCACCAAGAAAGCCAGCACTGACCTGCTGGCGGATATCGGGGGAG
ACCCCTTTGCTGCTCCCCAGGTGGTGCCAGCCTTTGCCTCATTCCCAGGCTTTGGAGTAGGCCAGACTCC
TGCCCATGGAGGCTTTGCCAACTTCGATGCCTTCAGCAGCAGCCCTAGCTCTTCCACCTTCGGAAGCCTC
CCTCCATCCGTCCAAGCGCCATTCCAGGCCCAGCCGACCCCTGCAGGAAGTGGCCAGATGTCTGCGTTTG
GTGTGGCACCCCTTGCAGCTGCCAGTCAACCCAACAACCTTGCAGATGTGGGCGGCCTCCTGGGTCCCAG
GATGGCTGCTGGAGGTCTCCCTGGCAGTGTTTTTGGGATGCCGAGCCAGGTTCCTGCCCTGCAGTCGGCC
GTGCCAGGTGTTAGCGGCAGTGGAGGGCTCCCCTTTGGAGCCTACACCAACCCCTTCGCCACCCCTGCCC
AAGCCCAGCTGCCTTCTACCAACCCATTCCAACCCAATGGTCTAGCCTCAGGGCCTGGCTTTGGGATGAG
CAGTGTTCGGCCTGGCCTTCTCCAGCCAGTGCCACCCTCCGGGGCCTTTGCCAGTCCCTTCTCTGCACCC
GTGTTCCCCACACAGGCTGGACTGGCCGACCAGCAGAATGGATCTTCCTTTGGCGACTTGGGGACCTCTA
AGCTGGGGCAGAGGCCACTGAGCCAGCCGGCTGGGATCTCTACCAATCCTTTCATGACTGGATCCTCAGC
GTTTGCCTCCAAACCTCCAACCACAAACCCATTCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207679 protein sequence
Red=Cloning site Green=Tags(s)

MVMAAKKGPGPGGGVGGSKAEAEAASEVWCRRVRELGGCSQAGNRHCFECAQRGVTYVDITVGSFVCTTC
SGLLRGLNPPHRVKSISMTTFTEPEVLFLQSRGNEVCRKIWLGLFDARTSLIPDSRDPQKVKEFLQEKYE
KKRWYVPPEQVKGPSYSKGSVSATPVQGSVPEGKPIRTLLGDPVPSLSDPASTSSQPGSQSQARSSSQAR
SSQPPSHSSTKKASTDLLADIGGDPFAAPQVVPAFASFPGFGVGQTPAHGGFANFDAFSSSPSSSTFGSL
PPSVQAPFQAQPTPAGSGQMSAFGVAPLAAASQPNNLADVGGLLGPRMAAGGLPGSVFGMPSQVPALQSA
VPGVSGSGGLPFGAYTNPFATPAQAQLPSTNPFQPNGLASGPGFGMSSVRPGLLQPVPPSGAFASPFSAP
VFPTQAGLADQQNGSSFGDLGTSKLGQRPLSQPAGISTNPFMTGSSAFASKPPTTNPFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178162
ORF Size 1437 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178162.3, NP_835456.1
RefSeq Size 2409 bp
RefSeq ORF 1440 bp
Locus ID 231801
UniProt ID Q80WC7
Cytogenetics 5 G2
MW 49 kDa
Summary This gene encodes a paralog of the HIV-1 Rev binding proteins that serve as cellular co-factors for HIV-1 Rev protein in shuttling viral pre-mRNAs from the nucleus to the cytoplasm. The encoded protein contains an ADP-ribosylation factor GTPase activating protein (Arf-GAP) zinc finger domain, several phenylalanine-glycine (FG) motifs and asparagine-proline-phenylalanine (NPF) motifs. Alternate splicing of this gene results in multiple transcript variants. provided by RefSeq, Dec 2014
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Documents
Resources
"NM_178162" in other vectors (4)
SKU Description Size Price
MC216605 Agfg2 (untagged) - Mouse ArfGAP with FG repeats 2 (Agfg2), transcript variant 2, (10ug) 10 ug
$503.00
MG207679 Agfg2 (tGFP-tagged) - Mouse HIV-1 Rev binding protein-like (Hrbl), transcript variant 2 10 ug
$657.00
MR207679L3 Lenti ORF clone of Agfg2 (Myc-DDK-tagged) - Mouse ArfGAP with FG repeats 2 (Agfg2), transcript variant 2 10 ug
$757.00
MR207679L4 Lenti ORF clone of Agfg2 (mGFP-tagged) - Mouse ArfGAP with FG repeats 2 (Agfg2), transcript variant 2 10 ug
$757.00

Plasmid Map for MR207679

Download sequence for

Cloning scheme for MR207679

Circular map for MR207679

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