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Pi4k2a (BC022127) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207674
Pi4k2a (Myc-DDK-tagged) - Mouse phosphatidylinositol 4-kinase type 2 alpha (cDNA clone MGC:37783 IMAGE:5097465)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pi4k2a
Synonyms MGC37783, Pi4k2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207674 representing BC022127
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGAGACGAGCCCGCTAGTGTCCCCCGAGCGGGCCCAACCCCCGGAGTACACCTTCCCGTCGGGCT
CCGGAGCTCACTTTCCGCAAGTACCGGGGGGCGCGGTCCGCGTGGCGGCGGCGGCCGGCTCCGGCCCGTC
ACCGCCGTGCTCGCCCGGCCACGACCGGGAGCGGCAGCCCCTGCTGGACCGGGCCCGGGGCGCGGCGGCG
CAGGGCCAGACCCACACGGTGGCGGTGCAGGCCCAGGCCCTGGCCGCCCAGGCGGCCGTGGCGGCGCACG
CCGTTCAGACCCACCGCGAGCGGAACGACTTCCCGGAGGACCCCGAGTTCGAGGTGGTGGTGCGGCAGGC
CGAGGTTGCCATCGAGTGCAGCATCTATCCCGAGCGCATCTACCAGGGCTCCAGTGGAAGCTACTTCGTC
AAGGACTCTCAGGGGAGAATCGTTGCTGTCTTCAAACCCAAGAATGAAGAGCCATATGGGCACCTTAACC
CTAAGTGGACCAAGTGGCTGCAGAAGCTATGCTGCCCCTGCTGCTTCGGCCGAGACTGCCTTGTTCTCAA
CCAGGGCTATCTCTCAGAGGCAGGGGCTAGCCTGGTGGACCAAAAACTGGAACTCAACATTGTACCACGT
ACAAAGGTAGTATACCTGGCCAGTGAAACCTTCAACTACAGTGCCATTGACCGAGTAAAGTCCAGGGGCA
AGCGGCTTGCACTAGAGAAAGTGCCAAAAGTTGGGCAGCGGTTTAACCGAATCGGCCTGCCACCAAAGGT
CGGGTCATTCCAGCTTTTCGTTGAAGGCTACAAAGATGCAGACTATTGGCTGCGGCGTTTTGAAGCAGAA
CCTCTCCCTGAGAACACGAACCGACAGCTGCTATTGCAGTTTGAGCGGTTGGTGGTCCTGGACTACATCA
TCCGCAACACTGACCGAGGCAATGACAACTGGTTGATCAAATATGACTGTCCGATGGATAATTCTAGCTG
TCGGGACACAGATTGGGTGATGGTGAGGGAGCCTGTTATCAAGGTGGCTGCCATAGACAACGGGCTAGCT
TTCCCACTGAAGCATCCTGACTCCTGGAGGGCATATCCTTTTTACTGGGCCTGGCTGCCTCAGGCGAAAG
TCCCGTTCTCTCAGGAGATCAAAGATTTGATTCTTCCAAAGATTTCAGACCCTAACTTCATCAAGGACTT
GGAGGAGGACCTATATGAACTCTTCAAGAGAGATCCTGGCTTCGACAGGGGCCAGTTCCATAAGCAGATT
GCTGTCATGAGAGGCCAGATCCTAAATTTGACCCAGGCCCTGAAAGACAATAAGAGCCCCCTGCACCTCG
TCCAGATGCCACCTGTGATTGTCGAGACGGCCCGCTCTCATCAGCGGTCTGCAAGCGAATCCTACACACA
GAGCTTTCAGAGTCGGAAGCCCTTCTTTTCATGGTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207674 representing BC022127
Red=Cloning site Green=Tags(s)

MDETSPLVSPERAQPPEYTFPSGSGAHFPQVPGGAVRVAAAAGSGPSPPCSPGHDRERQPLLDRARGAAA
QGQTHTVAVQAQALAAQAAVAAHAVQTHRERNDFPEDPEFEVVVRQAEVAIECSIYPERIYQGSSGSYFV
KDSQGRIVAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPR
TKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAE
PLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDNSSCRDTDWVMVREPVIKVAAIDNGLA
FPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFIKDLEEDLYELFKRDPGFDRGQFHKQI
AVMRGQILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSASESYTQSFQSRKPFFSWW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC022127
ORF Size 1437 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC022127
RefSeq Size 1960 bp
RefSeq ORF 1439 bp
Locus ID 84095
Cytogenetics 19 35.74 cM
MW 71.8 kDa
Summary Membrane-bound phosphatidylinositol-4 kinase (PI4-kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).UniProtKB/Swiss-Prot Function
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"BC022127" in other vectors (5)
SKU Description Size Price
MC206278 Pi4k2a (untagged) - Mouse phosphatidylinositol 4-kinase type 2 alpha (cDNA clone MGC:37783 IMAGE:5097465), (10ug) 10 ug
$457.00
MC218509 Pi4k2a (untagged) - Mouse phosphatidylinositol 4-kinase type 2 alpha (cDNA clone MGC:37783 IMAGE:5097465), (10ug) 10 ug
$503.00
MG207674 Pi4k2a (tGFP-tagged) - Mouse phosphatidylinositol 4-kinase type 2 alpha (cDNA clone MGC:37783 IMAGE:5097465) 10 ug
$657.00
MR207674L3 Lenti ORF clone of Pi4k2a (Myc-DDK-tagged) - Mouse phosphatidylinositol 4-kinase type 2 alpha (cDNA clone MGC:37783 IMAGE:5097465) 10 ug
$757.00
MR207674L4 Lenti ORF clone of Pi4k2a (mGFP-tagged) - Mouse phosphatidylinositol 4-kinase type 2 alpha (cDNA clone MGC:37783 IMAGE:5097465) 10 ug
$757.00

Plasmid Map for MR207674

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207674
Circular map for MR207674

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