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Sec61a1 (NM_016906) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207628
Sec61a1 (Myc-DDK-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Sec61a1 or NM_016906"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sec61a1
Synonyms AA408394; AA410007; Sec61a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207628 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGATCAAATTTCTGGAAGTCATCAAGCCATTCTGTGTCATCTTGCCGGAAATTCAGAAGCCAGAGA
GAAAGATTCAGTTTAAGGAGAAAGTTCTGTGGACAGCCATCACCCTCTTCATCTTCTTAGTATGCTGTCA
GATCCCCCTGTTTGGCATCATGTCTTCAGACTCAGCTGACCCGTTCTACTGGATGCGAGTGATTCTGGCC
TCTAACAGAGGTACCTTAATGGAGCTGGGCATCTCTCCTATTGTCACCTCCGGTCTTATCATGCAGCTCT
TAGCTGGTGCCAAGATAATTGAAGTTGGGGACACCCCGAAAGACCGAGCTCTCTTCAATGGAGCCCAGAA
GTTGTTTGGAATGATCATTACTATCGGTCAGTCCATTGTGTATGTGATGACGGGCATGTACGGGGACCCT
TCGGAGATGGGTGCTGGAATCTGCCTGCTGATCACCATTCAGCTCTTTGTTGCTGGCTTAATTGTCCTAC
TTTTGGATGAACTTCTGCAAAAAGGGTACGGCTTGGGCTCTGGCATTTCTCTCTTCATTGCGACTAACAT
CTGTGAGACCATTGTGTGGAAGGCATTCAGCCCCACCACTGTCAACACTGGCCGAGGGATGGAATTCGAA
GGCGCCATCATAGCGCTATTCCACCTGCTGGCCACCCGCACAGACAAGGTCCGAGCCCTTCGAGAGGCAT
TCTACCGCCAGAACCTCCCCAACCTCATGAACCTGATTGCCACCATCTTCGTCTTTGCCGTTGTCATCTA
TTTCCAGGGCTTCCGAGTGGACCTTCCAATCAAGTCGGCGCGTTACCGAGGCCAGTACAACACCTATCCC
ATCAAGCTCTTCTACACCTCCAACATCCCCATCATCTTGCAGTCTGCTCTGGTGTCCAACCTGTATGTCA
TCTCCCAGATGCTGTCAGCACGCTTCAGTGGCAACCTGCTGGTTAGCCTGCTGGGCACCTGGTCGGATAC
ATCATCTGGGGGCCCAGCCCGAGCTTACCCCGTTGGTGGCCTGTGTTACTACCTGTCTCCTCCGGAATCG
TTCGGCTCAGTGCTGGAGGACCCTGTCCATGCGGTCGTGTACATCGTGTTCATGCTGGGCTCCTGTGCAT
TCTTCTCCAAAACATGGATCGAAGTCTCAGGTTCTTCTGCCAAAGATGTTGCAAAGCAGCTGAAGGAGCA
GCAGATGGTGATGAGGGGCCACAGGGAGACCTCCATGGTGCATGAGCTCAACCGGTACATCCCCACAGCC
GCAGCCTTTGGTGGGCTGTGCATTGGGGCCCTCTCTGTCCTGGCTGACTTCCTGGGTGCTATCGGGTCTG
GAACTGGGATCCTGCTTGCGGTCACCATCATCTACCAGTACTTTGAGATCTTCGTCAAGGAACAGAGCGA
GGTGGGCAGCATGGGAGCCCTTCTCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207628 protein sequence
Red=Cloning site Green=Tags(s)

MAIKFLEVIKPFCVILPEIQKPERKIQFKEKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFYWMRVILA
SNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQSIVYVMTGMYGDP
SEMGAGICLLITIQLFVAGLIVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVNTGRGMEFE
GAIIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATIFVFAVVIYFQGFRVDLPIKSARYRGQYNTYP
IKLFYTSNIPIILQSALVSNLYVISQMLSARFSGNLLVSLLGTWSDTSSGGPARAYPVGGLCYYLSPPES
FGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTA
AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQSEVGSMGALLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016906
ORF Size 1428 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016906.4, NP_058602.1
RefSeq Size 3039 bp
RefSeq ORF 1431 bp
Locus ID 53421
UniProt ID P61620
Cytogenetics 6 D1
MW 52.3 kDa
Summary Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins. Controls the passive efflux of calcium ions from the ER lumen to the cytosol through SEC61 channel, contributing to the maintenance of cellular calcium homeostasis (By similarity). Plays a critical role in nephrogenesis, specifically at pronephros stage (PubMed:27392076).UniProtKB/Swiss-Prot Function
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"NM_016906" in other vectors (4)
SKU Description Size Price
MC216417 Sec61a1 (untagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1), (10ug) 10 ug
$503.00
MG207628 Sec61a1 (tGFP-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1) 10 ug
$657.00
MR207628L3 Lenti ORF clone of Sec61a1 (Myc-DDK-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1) 10 ug
$757.00
MR207628L4 Lenti ORF clone of Sec61a1 (mGFP-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1) 10 ug
$757.00

Plasmid Map for MR207628

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207628
Circular map for MR207628

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