Cacnb1 (BC077713) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207526
Cacnb1 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, beta 1 subunit (cDNA clone MGC:69994 IMAGE:30288369)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cacnb1
Synonyms CAB1; Cchb1; Cchlb1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207526 representing BC077713
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCCAGAAGAGCGGCATGTCCCGGGGCCCTTACCCACCTTCCCAAGAGATCCCTATGGAGGTCTTCG
ACCCCAGCCCACAGGGCAAGTACAGCAAGAGGAAAGGGCGGTTCAAAAGGTCAGACGGGAGTACGTCCTC
GGATACAACATCCAACAGCTTCGTCCGCCAGGGCTCAGCAGAGTCCTACACGAGCCGACCATCAGACTCT
GATGTGTCTCTGGAGGAGGACCGGGAAGCCTTAAGGAAGGAGGCAGAGCGCCAGGCCTTAGCCCAGCTCG
AGAAAGCCAAGACCAAACCAGTGGCTTTTGCTGTTCGGACAAATGTTGGCTACAATCCGTCTCCAGGGGA
TGAGGTGCCTGTACAGGGAGTGGCCATCACCTTTGAGCCCAAGGACTTCCTACACATCAAGGAGAAGTAC
AATAATGACTGGTGGATTGGGCGGCTGGTGAAGGAAGGCTGCGAGGTTGGCTTCATCCCCAGCCCGGTCA
AACTGGACAGCCTTCGTCTGCTGCAGGAACAGACCCTGCGCCAGAACCGCCTCAGCTCCAGCAAGTCAGG
TGACAACTCCAGTTCCAGTCTGGGAGATGTGGTGACTGGCACCCGCCGCCCCACACCCCCTGCCAGTGAG
CACGTGCCCCCCTATGACGTGGTGCCTTCCATGAGGCCCATCATCCTGGTGGGACCGTCGCTCAAGGGCT
ATGAGGTGACAGACATGATGCAGAAAGCGTTGTTTGACTTCCTCAAGCATCGGTTTGATGGCAGGATTTC
CATCACCCGGGTAACAGCTGACATTTCCCTGGCCAAACGCTCCGTCCTCAACAACCCCAGCAAACACATC
ATCATTGAGCCCTCCAACACGCGCTCCAGCCTGGCTGAGGTACAGAGTGAAATTGAGAGGATCTTCGAGC
TGGCCCGGACCTTGCAGCTGGTCGCCTTGGACGCTGACACCATCAACCACCCAGCCCAGCTCTCTAAAAC
GTCGCTGGCCCCCATCATTGTTTACATCAAGATCACATCTCCCAAGGTACTGCAGAGGCTCATCAAATCC
CGAGGGAAGTCTCAATCCAAACACCTCAATGTCCAAATAGCAGCCTCGGAGAAGCTGGCACAGTGTCCCC
CCGAAATGTTCGACATAATCCTGGACGAGAACCAATTGGAAGATGCCTGCGAGCACCTGGCTGAGTACTT
GGAAGCCTACTGGAAGGCCACACATCCGCCTAGCAGCACGCCACCCAATCCGCTGCTGAACCGCACCATG
GCTACCGCAGCTCTGGCTGCCAGCCCTGCCCCCGTCTCCAACCTCCAGGTACAGGTGCTCACCTCGCTCA
GGAGAAATCTCAGCTTCTGGGGCGGGCTGGAGGCCTCACCGCGGGGAGGCGACGCGGTGGCCCAGCCTCA
GGAGCACGCCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207526 representing BC077713
Red=Cloning site Green=Tags(s)

MVQKSGMSRGPYPPSQEIPMEVFDPSPQGKYSKRKGRFKRSDGSTSSDTTSNSFVRQGSAESYTSRPSDS
DVSLEEDREALRKEAERQALAQLEKAKTKPVAFAVRTNVGYNPSPGDEVPVQGVAITFEPKDFLHIKEKY
NNDWWIGRLVKEGCEVGFIPSPVKLDSLRLLQEQTLRQNRLSSSKSGDNSSSSLGDVVTGTRRPTPPASE
HVPPYDVVPSMRPIILVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSKHI
IIEPSNTRSSLAEVQSEIERIFELARTLQLVALDADTINHPAQLSKTSLAPIIVYIKITSPKVLQRLIKS
RGKSQSKHLNVQIAASEKLAQCPPEMFDIILDENQLEDACEHLAEYLEAYWKATHPPSSTPPNPLLNRTM
ATAALAASPAPVSNLQVQVLTSLRRNLSFWGGLEASPRGGDAVAQPQEHAM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC077713
ORF Size 1413 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC077713.1
RefSeq Size 1725 bp
RefSeq ORF 1415 bp
Locus ID 12295
Cytogenetics 11 61.5 cM
MW 63.3 kDa
Summary Regulatory subunit of L-type calcium channels. Regulates the activity of L-type calcium channels that contain CACNA1A as pore-forming subunit (By similarity). Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit and increases the presence of the channel complex at the cell membrane. Required for functional expression L-type calcium channels that contain CACNA1D as pore-forming subunit. Regulates the activity of L-type calcium channels that contain CACNA1B as pore-forming subunit (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC077713" in other vectors (4)
SKU Description Size Price
MC218143 Cacnb1 (untagged) - Mouse calcium channel, voltage-dependent, beta 1 subunit (cDNA clone MGC:69994 IMAGE:30288369), (10ug) 10 ug
$732.00
MG207526 Cacnb1 (tGFP-tagged) - Mouse calcium channel, voltage-dependent, beta 1 subunit (cDNA clone MGC:69994 IMAGE:30288369) 10 ug
$886.00
MR207526L3 Lenti ORF clone of Cacnb1 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, beta 1 subunit (cDNA clone MGC:69994 IMAGE:30288369) 10 ug
$986.00
MR207526L4 Lenti ORF clone of Cacnb1 (mGFP-tagged) - Mouse calcium channel, voltage-dependent, beta 1 subunit (cDNA clone MGC:69994 IMAGE:30288369) 10 ug
$986.00

Plasmid Map for MR207526

Download sequence for

Cloning scheme for MR207526

Circular map for MR207526

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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