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Ighg1 (BC024405) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207509
Ighg1 (Myc-DDK-tagged) - Mouse immunoglobulin heavy constant gamma 1 (G1m marker) (cDNA clone MGC:36307 IMAGE:5039226)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ighg1
Synonyms IgG1, VH7183
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207509 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTTGTGGCTGAACTGGATTTTCCTTGTAACACTTTTAAATGGTATCCAGTGTGAGGTGAATCTGG
TGGAGTCTGGAGGAGGCTTGGTACAGCCTGGGGGTTCTCTGCGTCTCTCCTGTGCAGCTTCTGGATTCAC
CTTTACTGATTACTACATGAGCTGGGTCCGCCAGCCTCCAGGGAAGGCACTTGAGTGGTTGGGTTTTATT
AGAAACAAAGCTAATGGTTACACAACAGAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAG
ATAATTCCCAAAGCATCCTCTATCTTCAAATGAATGCCCTGAGAGCTGAGGACAGTGCCACTTATTACTG
TGCAAGAGATAGGAGGAGTTCTTATTACTACAGTGGTACCTCCTTTGCTTACTGGGGCCAAGGGACTCTG
GTCACTGTCTCTGCAGCCAAAACGACACCCCCATCTGTCTATCCACTGGCCCCTGGATCTGCTGCCCAAA
CTAACTCCATGGTGACCCTGGGATGCCTGGTCAAGGGCTATTTCCCTGAGCCAGTGACAGTGACCTGGAA
CTCTGGATCCCTGTCCAGCGGTGTGCACACCTTCCCAGCTGTCCTGCAGTCTGACCTCTACACTCTGAGC
AGCTCAGTGACTGTCCCCTCCAGCACCTGGCCCAGCCAGACCGTCACCTGCAACGTTGCCCACCCGGCCA
GCAGCACCAAGGTGGACAAGAAAATTGTGCCCAGGGATTGTGGTTGTAAGCCTTGCATATGTACAGTCCC
AGAAGTATCATCTGTCTTCATCTTCCCCCCAAAGCCCAAGGATGTGCTCACCATTACTCTGACTCCTAAG
GTCACGTGTGTTGTGGTAGACATCAGCAAGGATGATCCCGAGGTCCAGTTCAGCTGGTTTGTAGATGATG
TGGAGGTGCACACAGCTCAGACAAAACCCCGGGAGGAGCAGTTCAACAGCACTTTCCGTTCAGTCAGTGA
ACTTCCCATCATGCACCAGGACTGGCTCAATGGCAAGGAGTTCAAATGCAGGGTCAACAGTGCAGCTTTC
CCTGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGCAGACCGAAGGCTCCACAGGTGTACACCATTC
CACCTCCCAAGGAGCAGATGGCCAAGGATAAAGTCAGTCTGACCTGCATGATAACAGACTTCTTCCCTGA
AGACATTACTGTGGAGTGGCAGTGGAATGGGCAGCCAGCGGAGAACTACAAGAACACTCAGCCCATCATG
GACACAGATGGCTCTTACTTCGTCTACAGCAAGCTCAATGTGCAGAAGAGCAACTGGGAGGCAGGAAATA
CTTTCACCTGCTCTGTGTTACATGAGGGCCTGCACAACCACCATACTGAGAAGAGCCTCTCCCACTCTCC
TGGTAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207509 protein sequence
Red=Cloning site Green=Tags(s)

MKLWLNWIFLVTLLNGIQCEVNLVESGGGLVQPGGSLRLSCAASGFTFTDYYMSWVRQPPGKALEWLGFI
RNKANGYTTEYSASVKGRFTISRDNSQSILYLQMNALRAEDSATYYCARDRRSSYYYSGTSFAYWGQGTL
VTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLS
SSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPK
VTCVVVDISKDDPEVQFSWFVDDVEVHTAQTKPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAF
PAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIM
DTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC024405
ORF Size 1407 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC024405
RefSeq Size 1643 bp
RefSeq ORF 1409 bp
Locus ID 16017
Cytogenetics 12 62.1 cM
MW 52 kDa
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"BC024405" in other vectors (4)
SKU Description Size Price
MC217711 Ighg1 (untagged) - Mouse immunoglobulin heavy constant gamma 1 (G1m marker) (cDNA clone MGC:36307 IMAGE:5039226), (10ug) 10 ug
$503.00
MG207509 Ighg1 (tGFP-tagged) - Mouse immunoglobulin heavy constant gamma 1 (G1m marker) (cDNA clone MGC:36307 IMAGE:5039226) 10 ug
$657.00
MR207509L3 Lenti ORF clone of Ighg1 (Myc-DDK-tagged) - Mouse immunoglobulin heavy constant gamma 1 (G1m marker) (cDNA clone MGC:36307 IMAGE:5039226) 10 ug
$757.00
MR207509L4 Lenti ORF clone of Ighg1 (mGFP-tagged) - Mouse immunoglobulin heavy constant gamma 1 (G1m marker) (cDNA clone MGC:36307 IMAGE:5039226) 10 ug
$757.00

Plasmid Map for MR207509

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207509
Circular map for MR207509

file-alt Citations

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