Vim (NM_011701) Mouse Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Vim |
| Synonyms | MGC102095 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_011701 |
| ORF Size | 1398 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011701.4 |
| RefSeq Size | 1834 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 22352 |
| UniProt ID | P20152 |
| Cytogenetics | 2 10.04 cM |
| MW | 53.7 kDa |
| Summary | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_011701" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC216239 | Vim (untagged) - Mouse vimentin (Vim), (10ug) | 10 ug |
$457.00
|
|
| MG207446 | Vim (tGFP-tagged) - Mouse vimentin (Vim) | 10 ug |
$886.00
|
|
| MR207446L3 | Lenti ORF clone of Vim (Myc-DDK-tagged) - Mouse vimentin (Vim) | 10 ug |
$986.00
|
|
| MR207446L4 | Lenti ORF clone of Vim (mGFP-tagged) - Mouse vimentin (Vim) | 10 ug |
$986.00
|
|
Citations
RNAi-mediated suppression of vimentin or glial fibrillary acidic protein prevents the establishment of Müller glial cell hypertrophy in progressive retinal degeneration
Hippert, Graca, Basche et al
Glia (2021)
Hippert, Graca, Basche et al
Glia (2021)
Cited by 30
AI Summary: OriGene contributed to this study by providing the Vimentin expression plasmid (pVimentin, MR207446), which was used to investigate the role of vimentin in Müller glial cell hypertrophy during retinal degeneration.
Snippet: Vimentin simultaneously. Gfap expression plasmid (pGfap) was generated by using pCR4-Gfap (Source Bioscience, Cambridge, UK) Vimentin expression plasmid (pVimentin) was purchased from Origene (MR207446). The;- Vimentin simultaneously. Gfap expression plasmid (pGfap) was generated by using pCR4-Gfap (Source Bioscience, Cambridge, UK) Vimentin expression plasmid (pVimentin) was purchased from Origene (MR207446). …
Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells
Wang, Sun, Wang et al
J. Neurovirol. (2019) 26 (1), 84-94 DOI: 10.1007/s13365-019-00800-8
Wang, Sun, Wang et al
J. Neurovirol. (2019) 26 (1), 84-94 DOI: 10.1007/s13365-019-00800-8
AI Summary: OriGene products, specifically the mouse vimentin cDNA plasmid (MR207446) and the control blank plasmid (PS100001), were used in this study to manipulate vimentin expression in brain endothelial cells, helping to elucidate the role of vimentin in blood-brain barrier permeability during Enterovirus A71 infection.
Vimentin Phosphorylation Underlies Myofibroblast Sensitivity to Withaferin A In Vitro and during Corneal Fibrosis
Bargagna-Mohan, Lei, Thompson et al
PLoS ONE (2015) 10 (7), e0133399 DOI: 10.1371/journal.pone.0133399
Bargagna-Mohan, Lei, Thompson et al
PLoS ONE (2015) 10 (7), e0133399 DOI: 10.1371/journal.pone.0133399
Cited by 35
AI Summary: OriGene contributed to this paper by providing the vimentin cDNA plasmid, which was used to study the role of vimentin in myofibroblast sensitivity to withaferin A during corneal fibrosis.
Snippet: The vimentin cDNA plasmid was purchased from Origene (Rockville, MD, USA), and antibodies for immunohistochemistry and western blot analysis were purchased from several companies and detail information are provided in each method section. ... Cite SaveSaving
Showing 1-3 of 3 papers.
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