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Sp100 (BC069183) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207411
Sp100 (Myc-DDK-tagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sp100
Synonyms A430075G10Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207411 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGGGAGCGACGGCAGCCCCAGGATGTCCACTGAACAGGAGAACACAGAGATGCATCTCATTGAAT
GTATGTTAAAGCACTTCAAGACACAGAAAGTGGCCATTTCTAATGCGATAAGAAGTACATTTCCTTTTCT
TGAGAGCCTCCGAGACCATGAATTCATCACTGGTAAAATGTATGAAGATTTGCTAGATTCCTGTAGATCG
CTGGTCCCTGTGGACAAAGTGATCTACAGAGCCCTGGAGGAACTGGAGAAGAAATTTGACATGACGGTAC
TGTGCGAATTGTTCAATGAGGTCAACATGGAGAAGTACCCCGATTTGAATCTCATTCGTAGAAGCTTTGG
ATGTGTGTTTCCCAATGAATTGTGTTTCCAAGGAATTGATGGAGGGAACCCAAACTCCCAGCTAAGTCTT
GAACAAGGACCTGGTGCCAGCTATTCTCAAGGAAGCCCGAATGGGTCATCCTTAGATCTCTCCGCATCTG
AAGGCTGGAGAAGTAATGATAGAAGAAATTCCAACCTCATGCAGGCAAACCAAACTGAGAATCATCAGCT
TGCCGAATCACCAGGTCACCTTGATTCCTGTGAACTCCAAGTCCAACTGAATAACAGAGATGCTACCCCA
GAGTCTTGCAGCCTATTGCCTCAGAATGAAGAAAGAGCTGTGCAACTCAACTATGAACTCCAGATTAATC
CCTGTTTTGTGCAACTGGTAGATGTAAAGAAGGAAAATTCATCATTTTCCTTGGCTGGTAACCAACAAAC
CCGAGCAAGGACCAACCAAAATGAGGACTCTGAGATAATAGAACTCAGCAGTGGGGATTCTGATAATGGA
GAGAACTTCTCAGAAGCTACAACCACAGTCCCCTCCCAGCCAGCACCTGCATATTCCAGAAAACCTCCAA
CACTGAGAAGAGACAGAGGAGGAGGGAAAAAGAGAGAGAGACTTGGAAGCTACTTAATACGGAACATCAA
AATCCCAATGAAACCAAGCTGGAAAACAGCTTTCCTAGCAAGAAGTGCCAATCCTTCTTCACAACGACGC
AGAAAAAGAGGTCCGAGAATCCCCAGAGAAGAAAATGCGGATTTTGGAGGAGCTGAGCTTCCTGTGGTAT
GTGGCAACGCTCAGGGCTTCCTGGATAAGGAGAAATTTAAGCAAGGCATCTATGTGAGGAGTATACGGGG
CAAGACAGGGCGGTTATTCACTCCCATGGACTTTGAAATCGAAGGAAACTGTGAAAAAGCCAAGAACTGG
CGGCAAAGCATCCGCTGCAAGGGATGGACCCTGAGAGAACTGATACAGAAAGGAGTACTACAAGACCCAC
CAAGGAAAAAGAAGGAAACTCCACGAAACCCAAGGCAAACAAGGAGACAGGTAAATGCCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207411 protein sequence
Red=Cloning site Green=Tags(s)

MEGSDGSPRMSTEQENTEMHLIECMLKHFKTQKVAISNAIRSTFPFLESLRDHEFITGKMYEDLLDSCRS
LVPVDKVIYRALEELEKKFDMTVLCELFNEVNMEKYPDLNLIRRSFGCVFPNELCFQGIDGGNPNSQLSL
EQGPGASYSQGSPNGSSLDLSASEGWRSNDRRNSNLMQANQTENHQLAESPGHLDSCELQVQLNNRDATP
ESCSLLPQNEERAVQLNYELQINPCFVQLVDVKKENSSFSLAGNQQTRARTNQNEDSEIIELSSGDSDNG
ENFSEATTTVPSQPAPAYSRKPPTLRRDRGGGKKRERLGSYLIRNIKIPMKPSWKTAFLARSANPSSQRR
RKRGPRIPREENADFGGAELPVVCGNAQGFLDKEKFKQGIYVRSIRGKTGRLFTPMDFEIEGNCEKAKNW
RQSIRCKGWTLRELIQKGVLQDPPRKKKETPRNPRQTRRQVNAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC069183
ORF Size 1392 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC069183, AAH69183
RefSeq Size 1665 bp
RefSeq ORF 1394 bp
Locus ID 20684
Cytogenetics 1 43.6 cM
MW 52.6 kDa
Summary Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. May also play a role in infection by viruses through mechanisms that may involve chromatin and/or transcriptional regulation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"BC069183" in other vectors (4)
SKU Description Size Price
MC217750 Sp100 (untagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025), (10ug) 10 ug
$503.00
MG207411 Sp100 (tGFP-tagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025) 10 ug
$657.00
MR207411L3 Lenti ORF clone of Sp100 (Myc-DDK-tagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025) 10 ug
$757.00
MR207411L4 Lenti ORF clone of Sp100 (mGFP-tagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025) 10 ug
$757.00

Plasmid Map for MR207411

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207411
Circular map for MR207411

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