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Itpka (NM_146125) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207337
Itpka (Myc-DDK-tagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase A (Itpka)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Itpka
Synonyms MGC28924
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207337 representing NM_146125
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGCCCGGGCGCCCGACGGGCATGGCGCGGCCACGGGGCGCGGGGCCCTGCAGCCCCGGGCTGG
AGAGGGCTCCGCGCCGGAGCGTCGGGGAGCTGCGCCTGCTCTTCGAAGCGCGCTGCGCCGCAGTCGCCGC
TGCAGCAGCCGCAGGGGAGCCCCGGGCCCGCGGGGCCAAGCGGCGTGGGGGACAAGTGCCCAACGGGCTC
CCGCGTGCTGCCCCTGCCCCGGTGATCCCTCAGCTCACTGTGACAAGCGAGGAGGATGTGACCCCGGCCA
GCCCAGGGCCGCCAGACCAGGAGGGGAACTGGCTCCCAGCTGCGGGGTCGCACCTGCAGCAGCCACGCCG
CCTCTCCACCTCGTCCCTCTCCTCCACCGGCTCCTCGTCGCTGCTCGAGGACTCGGAGGACGATCTGCTG
AGCGACAGTGAGAGCAGGAGCCGCGGCAACGTGCAGCTGGAAACCAGCGAGGACGTGGGGCAGAAAAGCC
ACTGGCAGAAGATCCGTACCATGGTCAATCTGCCTGTCATGAGTCCTTTCAGAAAGCGCTACTCCTGGGT
GCAGTTAGCAGGGCACACAGGGAGTTTCAAAGCTGCCGGCACCAGTGGCCTGATCCTGAAACGCAGCTCG
GAGCCTGAACACTACTGCCTGGTGCGGCTGATGGCTGACGTGCTGCGTGGGTGTGTGCCGGCCTTCCATG
GCATAGTCGAGCGGGATGGTGAAAGCTACTTGCAGTTACAGGACCTGCTCGATGGCTTCGATGGGCCTTG
TGTGCTTGACTGCAAGATGGGTGTCAGAACTTACCTGGAAGAGGAGCTGACCAAAGCCCGAGAACGACCC
AAGCTGCGGAAGGACATGTATAAGAAGATGCTGGCAGTGGACCCTGAGGCACCTACTGAGGAGGAGCATG
CGCAGCGCGCCGTCACCAAACCACGCTACATGCAATGGCGCGAAGGCATCAGCTCCAGCACTACACTCGG
CTTTCGCATTGAGGGCATCAAGAAAGCCGATGGATCTTGCAGTACTGATTTCAAAACTACACGAAGCCGA
GAGCAAGTGACCCGTGTCTTTGAGGAGTTCATGCAAGGAGATGCAGAAGTGCTGAGGAGGTATCTGAACC
GCCTACAGCAGATCCGGGATACCCTGGAGATCTCTGATTTCTTTAGACGGCACGAGGTGATTGGCAGCTC
ACTCCTCTTCGTGCATGACCATTGCCATCGTGCTGGTGTGTGGCTCATCGATTTTGGCAAGACCACGCCT
CTCCCCGATGGCCAGATCCTGGATCATCGGAGGCCCTGGGAGGAGGGCAACCGTGAGGACGGCTATTTGC
TGGGGCTGGACAATCTCATTGGCATCTTGGCCAGCCTGGCTGAGAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207337 representing NM_146125
Red=Cloning site Green=Tags(s)

MTLPGRPTGMARPRGAGPCSPGLERAPRRSVGELRLLFEARCAAVAAAAAAGEPRARGAKRRGGQVPNGL
PRAAPAPVIPQLTVTSEEDVTPASPGPPDQEGNWLPAAGSHLQQPRRLSTSSLSSTGSSSLLEDSEDDLL
SDSESRSRGNVQLETSEDVGQKSHWQKIRTMVNLPVMSPFRKRYSWVQLAGHTGSFKAAGTSGLILKRSS
EPEHYCLVRLMADVLRGCVPAFHGIVERDGESYLQLQDLLDGFDGPCVLDCKMGVRTYLEEELTKARERP
KLRKDMYKKMLAVDPEAPTEEEHAQRAVTKPRYMQWREGISSSTTLGFRIEGIKKADGSCSTDFKTTRSR
EQVTRVFEEFMQGDAEVLRRYLNRLQQIRDTLEISDFFRRHEVIGSSLLFVHDHCHRAGVWLIDFGKTTP
LPDGQILDHRRPWEEGNREDGYLLGLDNLIGILASLAER

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146125
ORF Size 1377 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146125.2, NP_666237.1
RefSeq Size 1808 bp
RefSeq ORF 1380 bp
Locus ID 228550
UniProt ID Q8R071
Cytogenetics 2 E5
MW 50.9 kDa
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Documents
Resources
"NM_146125" in other vectors (3)
SKU Description Size Price
MC201507 Itpka (untagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase A (Itpka), (10ug) 10 ug
$457.00
MR207337L3 Lenti ORF clone of Itpka (Myc-DDK-tagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase A (Itpka) 10 ug
$757.00
MR207337L4 Lenti ORF clone of Itpka (mGFP-tagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase A (Itpka) 10 ug
$757.00

Plasmid Map for MR207337

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207337
Circular map for MR207337

file-alt Citations

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