OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Rrp8 (NM_025897) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR207304
Rrp8 (Myc-DDK-tagged) - Mouse ribosomal RNA processing 8, methyltransferase, homolog (yeast) (Rrp8), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
4 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Rrp8 or NM_025897"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rrp8
Synonyms 1500003O22Rik; 2900001K19Rik; AW538116
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207304 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCGAAGAGCCCGAATGGGTGGAGGCGGCCCCAGCCATCGTGGGCCTAGGGGCCGCGACAGCACAGG
TTAGGCCGGCGACTGCCCCGCCAGTCAAGGGACGCAAGCGCCGCCATCTCTTGGCCACATTACGGGCTCT
GGAAGCAGCATCTCTCTCCCAACAAACCCCCAGCCTACCTGGCAGTGACTCTGAGGAGGAGGAGGAGGTA
GGAAGGAAGAAGAGACACCTCCAAAGGCCCTCACTTGCCAGCGTCTCAAAGGAAGTAGGGAAGAAAAGAA
AAGGGAAATGTCAGAAACAGGCGCCATCCATCAGTGACTCTGAGGGGAAAGAAATAAGAAGAAAATGCCA
CAGACAAGCTCCTCCTCTTGGTGGGGTCTCTGCTGGAGAAGAAAAAGGAAAGAGAAAATGCCAGGAATAT
TCCTCTTTACACCTAACCCAGCCCCTGGACAGTGTTGACCAAACAGTTCACAATTCCAGGACGAGTACTG
CTACAATTGACCCATCCAAGCCAAGCCCTGAGTCTATGTCACCTAACTCCTCACACACCCTGAGCCGCAA
GCAGTGGCGGAACCGGCAGAAAAATAAGCGGAGACACAAGAACAAATTTCGGCCACTCCAGACACCAGAG
CAGGCTCCTCCCAAGGCTTCCATAGAGGAGACTGAGGTGCCTCCTGTCCCAAAGTCAGATAGTCAAGAGT
CTAGAGCTGGAGCCCTGCGAGCACGCATGACACAACGCCTGGATGGGGCCCGATTTCGCTACCTTAATGA
ACAGTTGTACTCAGGGCCCAGCAGTGCTGCCCGACGCCTATTCCAGGAAGACCCTGAGGCTTTTCTCCTT
TATCACCGTGGCTTCCAGAGACAAGTAAAGAAGTGGCCACTGCACCCAGTGGACCGTATTGCCAAAGATC
TCCGCCAGAAGCCTGCATCCTTAGTGGTAGCTGACTTTGGCTGTGGAGATTGCCGCCTAGCTTCAAGTGT
CCGGAACCCTGTGCACTGTTTTGATTTGGCTTCTCTGGACCCCAGGGTCACAGTATGTGACATGGCCCAG
GTGCCTCTGGAGGATGAATCTGTAGATGTGGCTGTGTTTTGCCTTTCACTGATGGGAACTAACATCAGGG
ACTTCCTTGAGGAGGCAAATCGAGTGCTAAAAACAGGGGGTCTTCTCAAAGTAGCTGAAGTCAGCAGCCG
CTTTGAGGATATTCGGACCTTTTTGGGGGCTGTGACCAAACTCGGCTTTAAGATTATCTACAAGGACCTG
ACCAACAGCCACTTCTTCTTGTTTGACTTTGAAAAGACCGGACCTCCTCGAGTAGGACCCAAAGCCCAAC
TCTCAGGCCTTAAACTTCAGCCCTGTCTCTACAAGCGCAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207304 protein sequence
Red=Cloning site Green=Tags(s)

MFEEPEWVEAAPAIVGLGAATAQVRPATAPPVKGRKRRHLLATLRALEAASLSQQTPSLPGSDSEEEEEV
GRKKRHLQRPSLASVSKEVGKKRKGKCQKQAPSISDSEGKEIRRKCHRQAPPLGGVSAGEEKGKRKCQEY
SSLHLTQPLDSVDQTVHNSRTSTATIDPSKPSPESMSPNSSHTLSRKQWRNRQKNKRRHKNKFRPLQTPE
QAPPKASIEETEVPPVPKSDSQESRAGALRARMTQRLDGARFRYLNEQLYSGPSSAARRLFQEDPEAFLL
YHRGFQRQVKKWPLHPVDRIAKDLRQKPASLVVADFGCGDCRLASSVRNPVHCFDLASLDPRVTVCDMAQ
VPLEDESVDVAVFCLSLMGTNIRDFLEEANRVLKTGGLLKVAEVSSRFEDIRTFLGAVTKLGFKIIYKDL
TNSHFFLFDFEKTGPPRVGPKAQLSGLKLQPCLYKRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025897
ORF Size 1371 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025897.2
RefSeq Size 2856 bp
RefSeq ORF 1374 bp
Locus ID 101867
UniProt ID Q9DB85
Cytogenetics 7 E3
MW 51.1 kDa
Summary Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_025897" in other vectors (4)
SKU Description Size Price
MC202542 Rrp8 (untagged) - Mouse ribosomal RNA processing 8, methyltransferase, homolog (yeast) (Rrp8), transcript variant 2, (10ug) 10 ug
$457.00
MG207304 Rrp8 (tGFP-tagged) - Mouse RIKEN cDNA 1500003O22 gene (1500003O22Rik) 10 ug
$657.00
MR207304L3 Lenti ORF clone of Rrp8 (Myc-DDK-tagged) - Mouse ribosomal RNA processing 8, methyltransferase, homolog (yeast) (Rrp8), transcript variant 2 10 ug
$757.00
MR207304L4 Lenti ORF clone of Rrp8 (mGFP-tagged) - Mouse ribosomal RNA processing 8, methyltransferase, homolog (yeast) (Rrp8), transcript variant 2 10 ug
$757.00

Plasmid Map for MR207304

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207304
Circular map for MR207304

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products