Psmd12 (NM_025894) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207290
Psmd12 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (Psmd12)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Psmd12
Synonyms 1500002F15Rik; AI480719; P55
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207290 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACGGTGGCTCGGAGCGGGCCGATGGGCGCATTGTGAAGATGGAAGTGGACTACAGCGCCACGG
TGGACCAGCGCCTGCCTGAGTGCGAGAAGCTGGCCAAGGAAGGGCGGCTTCAAGAAGTCATCGAAACCCT
TCTCTCTCTAGAAAAGCAGACCCGGACTGCCTCTGACATGGTGTCCACGTCCCGCATCTTGGTTGCAGTA
GTGAAAATGTGCTATGAGGCTAAAGAATGGGACTTACTTAATGAAAATATTATGCTTCTGTCAAAACGAC
GGAGTCAGTTAAAACAAGCAGTTGCAAAAATGGTTCAGCAGTGCTGTACTTACGTGGAGGAGATCACAGA
CCTGCCAGTCAAGCTGCGATTGATTGATACTCTGCGGATGGTTACAGAAGGAAAGATTTATGTTGAAATT
GAGCGTGCTCGGCTGACTAAAACCTTAGCAACTATTAAAGAGCAAAATGGCGATGTGAAAGAGGCTGCCT
CCATCCTTCAAGAGTTACAGGTGGAAACCTATGGGTCCATGGAGAAGAAGGAGCGAGTGGAATTCATTCT
GGAGCAGATGAGACTCTGCCTAGCTGTGAAGGATTACATTCGCACACAGATCATTAGCAAAAAAATTAAT
ACCAAATTCTTCCAAGAAGAAAACACAGAGAACTTGAAGTTGAAGTACTATAACTTAATGATTCAGCTGG
ATCAGCACGAGGGGTCCTATCTGTCCATCTGCAAACACTACAGAGCCATCTATGACACTCCCTGCATTCA
GGCAGAAAGTGACAAGTGGCAGCAGGCTCTGAAAAGTGTTGTTCTCTATGTTATCCTGGCTCCTTTTGAC
AATGAGCAATCAGATTTGGTTCACCGGATAAGCAGTGACAAGAAGCTGGAAGAAATTCCCAAATACAAGG
ATCTGCTGAAGCTTTTTACCACCATGGAGCTGATGCGCTGGTCCACTCTTGTTGAGGACTATGGGGTGGA
GCTGCGAAAAGGCTCCTCAGAGACCCCGGCGACTGACGTTTTCAGCTCTACAGAAGAAGGGGAAAAGAGG
TGGAAAGACTTGAAGAGCAGAGTGGTGGAGCACAACATCAGAATTATGGCCAAGTACTACACTCGGATAA
CAATGAAAAGGATGGCACAACTTCTGGATCTCTCTGTGGATGAGTCAGAGGCTTTCCTCTCGAATCTAGT
CGTTAACAAGACCATCTTTGCTAAAGTAGACAGGTTGGCTGGAGTCATCAACTTCCAGAGACCCAAGGAT
CCAAATAATTTATTAAATGACTGGTCTCAGAAACTGAACTCGCTGATGTCTCTGGTTAACAAAACGACAC
ACCTCATAGCCAAAGAGGAGATGATACATAATCTACAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207290 protein sequence
Red=Cloning site Green=Tags(s)

MADGGSERADGRIVKMEVDYSATVDQRLPECEKLAKEGRLQEVIETLLSLEKQTRTASDMVSTSRILVAV
VKMCYEAKEWDLLNENIMLLSKRRSQLKQAVAKMVQQCCTYVEEITDLPVKLRLIDTLRMVTEGKIYVEI
ERARLTKTLATIKEQNGDVKEAASILQELQVETYGSMEKKERVEFILEQMRLCLAVKDYIRTQIISKKIN
TKFFQEENTENLKLKYYNLMIQLDQHEGSYLSICKHYRAIYDTPCIQAESDKWQQALKSVVLYVILAPFD
NEQSDLVHRISSDKKLEEIPKYKDLLKLFTTMELMRWSTLVEDYGVELRKGSSETPATDVFSSTEEGEKR
WKDLKSRVVEHNIRIMAKYYTRITMKRMAQLLDLSVDESEAFLSNLVVNKTIFAKVDRLAGVINFQRPKD
PNNLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025894
ORF Size 1368 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025894.2
RefSeq Size 1723 bp
RefSeq ORF 1371 bp
Locus ID 66997
UniProt ID Q9D8W5
Cytogenetics 11 E1
MW 52.9 kDa
Summary Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025894" in other vectors (4)
SKU Description Size Price
MC200189 Psmd12 (untagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (Psmd12), (10ug) 10 ug
$457.00
MG207290 Psmd12 (tGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (Psmd12) 10 ug
$657.00
MR207290L3 Lenti ORF clone of Psmd12 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (Psmd12) 10 ug
$757.00
MR207290L4 Lenti ORF clone of Psmd12 (mGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (Psmd12) 10 ug
$757.00

Plasmid Map for MR207290

Download sequence for

Cloning scheme for MR207290

Circular map for MR207290

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