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Adat1 (BC019976) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207245
Adat1 (Myc-DDK-tagged) - Mouse adenosine deaminase, tRNA-specific 1 (cDNA clone MGC:28961 IMAGE:4458235)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Adat1 or BC019976"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adat1
Synonyms mADAT1, MMADAT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207245 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGACCGCGGATGAGATCGCTCAGCTGTGCTATGCACACTATAACGTCAGACTGCCCAAGCAGGGGA
AGCCTGAGCCAAATCGTGAGTGGACCTTGCTGGCAGCTGTGGTGAAGATTCAGGCTTCAGCTAACCAAGC
TTGTGACATCCCTGAGAAGGAAGTGCAAGTGACAAAGGAAGTTGTCTCAATGGGAACAGGAACAAAATGC
ATAGGCCAGTCCAAGATGCGAGAGAGCGGGGACATCCTCAATGACAGCCATGCTGAGATCATAGCCAGAA
GGAGTTTCCAGAGATACCTTCTCCATCAGCTCCATTTGGCAGCCGTCCTGAAAGAGGATAGCATCTTTGT
TCCAGGGACCCAAAGAGGACTGTGGAGGCTCAGACCTGACCTGTCGTTTGTGTTTTTCTCTAGCCACACA
CCCTGTGGGGATGCCTCCATCATCCCGATGCTCGAGTTTGAAGAACAGCCTTGCTGTCCTGTCATCAGAA
GTTGGGCCAACAACTCACCTGTACAAGAGACTGAGAATCTAGAGGATTCTAAAGATAAAAGGAACTGTGA
AGATCCCGCCAGTCCTGTAGCCAAGAAGATGAGGCTGGGAACACCTGCCAGGTCACTCTCCAATTGTGTA
GCTCACCATGGGACCCAGGAAAGTGGTCCAGTCAAACCAGATGTCAGCAGCTCTGATCTCACCAAGGAGG
AACCAGATGCTGCCAATGGAATAGCTTCAGGTAGCTTCAGAGTGGTGGATGTGTACAGGACGGGAGCCAA
GTGTGTTCCTGGAGAAACTGGAGACTTGAGAGAGCCAGGTGCTGCCTACCACCAGGTGGGGCTACTTCGT
GTAAAACCTGGCAGGGGAGACAGGACATGCTCCATGTCCTGCAGTGACAAGATGGCAAGGTGGAATGTCC
TCGGATGCCAGGGCGCACTGCTGATGCACTTCCTGGAGAAGCCTATCTACCTGTCAGCTGTGGTCATCGG
GAAGTGCCCGTACAGCCAGGAAGCCATGAGGAGAGCATTGACTGGCAGGTGTGAGGAAACCTTGGTTTTA
CCCAGAGGCTTTGGAGTTCAAGAACTGGAAATACAGCAGTCGGGTTTACTGTTTGAACAGAGCCGCTGTG
CGGTGCACAGGAAAAGAGGTGACAGTCCAGGCCGACTCGTCCCCTGTGGGGCAGCTATCAGCTGGAGCGC
TGTCCCTCAGCAGCCCTTGGATGTTACAGCCAATGGTTTTCCACAGGGAACAACAAAGAAAGAGATTGGA
AGCCCTAGGGCCAGATCCCGAATCAGCAAGGTGGAACTCTTTAGATCATTCCAGAAGCTGCTGAGCAGCA
TTGCAGATGACGAGCAGCCAGACTCCATCAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207245 protein sequence
Red=Cloning site Green=Tags(s)

MWTADEIAQLCYAHYNVRLPKQGKPEPNREWTLLAAVVKIQASANQACDIPEKEVQVTKEVVSMGTGTKC
IGQSKMRESGDILNDSHAEIIARRSFQRYLLHQLHLAAVLKEDSIFVPGTQRGLWRLRPDLSFVFFSSHT
PCGDASIIPMLEFEEQPCCPVIRSWANNSPVQETENLEDSKDKRNCEDPASPVAKKMRLGTPARSLSNCV
AHHGTQESGPVKPDVSSSDLTKEEPDAANGIASGSFRVVDVYRTGAKCVPGETGDLREPGAAYHQVGLLR
VKPGRGDRTCSMSCSDKMARWNVLGCQGALLMHFLEKPIYLSAVVIGKCPYSQEAMRRALTGRCEETLVL
PRGFGVQELEIQQSGLLFEQSRCAVHRKRGDSPGRLVPCGAAISWSAVPQQPLDVTANGFPQGTTKKEIG
SPRARSRISKVELFRSFQKLLSSIADDEQPDSIR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC019976
ORF Size 1362 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019976, AAH19976
RefSeq Size 2036 bp
RefSeq ORF 1364 bp
Locus ID 30947
Cytogenetics 8 E1
MW 50 kDa
Summary Specifically deaminates adenosine-37 to inosine in tRNA-Ala.UniProtKB/Swiss-Prot Function
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"BC019976" in other vectors (4)
SKU Description Size Price
MC217786 Adat1 (untagged) - Mouse adenosine deaminase, tRNA-specific 1 (cDNA clone MGC:28961 IMAGE:4458235), (10ug) 10 ug
$503.00
MG207245 Adat1 (tGFP-tagged) - Mouse adenosine deaminase, tRNA-specific 1 (cDNA clone MGC:28961 IMAGE:4458235) 10 ug
$657.00
MR207245L3 Lenti ORF clone of Adat1 (Myc-DDK-tagged) - Mouse adenosine deaminase, tRNA-specific 1 (cDNA clone MGC:28961 IMAGE:4458235) 10 ug
$757.00
MR207245L4 Lenti ORF clone of Adat1 (mGFP-tagged) - Mouse adenosine deaminase, tRNA-specific 1 (cDNA clone MGC:28961 IMAGE:4458235) 10 ug
$757.00

Plasmid Map for MR207245

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207245
Circular map for MR207245

file-alt Citations

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