Idh2 (NM_173011) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207208
Idh2 (Myc-DDK-tagged) - Mouse isocitrate dehydrogenase 2 (NADP+), mitochondrial (Idh2), nuclear gene encoding mitochondrial protein
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Idh2
Synonyms E430004F23; Idh-2; IDPm
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207208 representing NM_173011
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCTACCTGCGGGCTGTGAGCTCGCTCTGCAGAGCCTCGGGCTCAGCGCGGACCTGGGCACCGG
CAGCACTGACTGTCCCCAGCTGGCCGGAGCAGCCGCGGCGCCACTATGCTGAGAAGAGGATCAAGGTGGA
GAAGCCGGTAGTGGAGATGGACGGTGACGAGATGACCCGGATCATCTGGCAGTTCATCAAGGAGAAGCTC
ATCCTGCCTCACGTGGATGTTCAGCTCAAGTATTTTGACCTTGGGCTTCCAAACCGTGACCAGACCAATG
ACCAGGTCACCATTGACTCTGCTCTGGCCACCCAGAAGTACAGTGTGGCTGTCAAGTGTGCCACAATCAC
CCCTGATGAGGCCCGTGTGGAAGAGTTCAAGCTGAAGAAAATGTGGAAGAGCCCTAACGGAACGATCCGG
AACATCCTTGGGGGAACCGTCTTCAGAGAGCCAATCATCTGCAAAAACATCCCCCGCCTTGTCCCTGGCT
GGACCAAGCCCATCACCATTGGCAGGCACGCCCATGGCGACCAGTACAAGGCCACAGATTTTGTGGTAGA
TCGAGCTGGCACGTTCAAGTTGGTCTTCACCCCAAAGGATGGCAGCAGTGCCAAGGAGTGGGAGGTGTAT
AACTTCCCTGCCGGAGGCGTTGGGATGGGGATGTACAACACCGACGAGTCCATTTCGGGCTTCGCGCACA
GCTGCTTCCAGTACTCTATCCAGAAGAAATGGCCGCTCTACTTGAGCACCAAGAACACCATTCTGAAGGC
CTATGACGGGCGTTTCAAGGACATCTTCCAGGAGATCTTTGACAAGCACTATAAGACTGACTTCGACAAG
AATAAGATCTGGTATGAACATCGGCTCATCGACGACATGGTGGCCCAGGTGCTCAAGTCTTCCGGTGGCT
TTGTGTGGGCTTGCAAAAACTATGATGGAGACGTGCAGTCTGACATCCTGGCTCAAGGCTTTGGCTCCCT
CGGCCTGATGACATCTGTGCTGGTCTGCCCTGATGGGAAGACAATTGAGGCTGAGGCTGCTCATGGGACA
GTCACCCGCCATTACCGAGAACACCAGAAGGGCCGGCCCACCAGTACCAACCCTATTGCCAGCATCTTTG
CCTGGACACGGGGTCTGGAGCATCGTGGGAAGCTGGATGGGAACCAGGACCTTATCAGGTTTGCACAGAC
GCTGGAGAAGGTGTGCGTGCAGACTGTGGAGAGCGGAGCGATGACCAAGGACCTGGCTGGCTGTATCCAT
GGCCTCAGCAATGTGAAGCTGAACGAGCACTTCCTGAACACCACAGACTTCCTGGACACCATTAAGAGCA
ACCTGGACAGAGCTCTGGGCAAGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207208 representing NM_173011
Red=Cloning site Green=Tags(s)

MAGYLRAVSSLCRASGSARTWAPAALTVPSWPEQPRRHYAEKRIKVEKPVVEMDGDEMTRIIWQFIKEKL
ILPHVDVQLKYFDLGLPNRDQTNDQVTIDSALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIR
NILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVVDRAGTFKLVFTPKDGSSAKEWEVY
NFPAGGVGMGMYNTDESISGFAHSCFQYSIQKKWPLYLSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDK
NKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGT
VTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQTLEKVCVQTVESGAMTKDLAGCIH
GLSNVKLNEHFLNTTDFLDTIKSNLDRALGKQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173011
ORF Size 1356 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173011.2, NP_766599.2
RefSeq Size 1718 bp
RefSeq ORF 1359 bp
Locus ID 269951
UniProt ID P54071
Cytogenetics 7 45.43 cM
MW 51.4 kDa
Summary Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_173011" in other vectors (4)
SKU Description Size Price
MC216061 Idh2 (untagged) - Mouse isocitrate dehydrogenase 2 (NADP+), mitochondrial (Idh2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$732.00
MG207208 Idh2 (tGFP-tagged) - Mouse isocitrate dehydrogenase 2 (NADP+), mitochondrial (Idh2) 10 ug
$886.00
MR207208L3 Lenti ORF clone of Idh2 (Myc-DDK-tagged) - Mouse isocitrate dehydrogenase 2 (NADP+), mitochondrial (Idh2), nuclear gene encoding mitochondrial protein 10 ug
$986.00
MR207208L4 Lenti ORF clone of Idh2 (mGFP-tagged) - Mouse isocitrate dehydrogenase 2 (NADP+), mitochondrial (Idh2), nuclear gene encoding mitochondrial protein 10 ug
$986.00

Plasmid Map for MR207208

Download sequence for

Cloning scheme for MR207208

Circular map for MR207208

file-alt Citations

Adenylate cyclase 10 promotes brown adipose tissue thermogenesis
Das, Mund, Hagag et al
iScience (2025) 28 (2), 111833 DOI: 10.1016/j.isci.2025.111833
Cited by 5
AI Summary: OriGene products, including the Idh2 (MR207208), Idh3a (MR205632), and Adcy10 (MR217736) Mouse Tagged ORF Clones, were used in this study to investigate the roles of these genes in regulating mitochondrial membrane potential and thermogenesis in brown adipose tissue.
Snippet: Idh2 (NM_173011) Mouse Tagged ORF Clone Origene MR207208 Idh3a (NM_029573) Mouse Tagged ORF Clone Origene MR205632 Adcy10 (NM_173029) Mouse Tagged ORF Clone Origene MR217736
FigureFigureFigure
Showing 1-1 of 1 papers.
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