Tubg2 (NM_134028) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207203
Tubg2 (Myc-DDK-tagged) - Mouse tubulin, gamma 2 (Tubg2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tubg2
Synonyms AI504772; Tubgl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207203 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCGGGAGATCATCACCCTGCAGCTGGGCCAGTGCGGCAACCAGATTGGGTTCGAGTTCTGGAAAC
AGCTGTGTGCTGAACATGGAATCAGCCCCGAGGGCATCGTAGAGGAGTTCGCCACCGAGGGCACTGACCG
AAAGGATGTCTTTTTCTACCAGGCAGATGATGAGCACTACATCCCCCGGGCTGTGCTGCTGGACCTGGAG
CCCCGGGTGATCCATTCCATCCTCAACTCCTCCTATGCTAAGCTCTACAACCCGGAGAACATCTACCTGT
CGGAGCATGGCGGAGGAGCTGGCAACAACTGGGGCAGAGGATTCTCCCAGGGTGAGAAAATTCACGAGGA
CATCTTTGACATTATAGACCGAGAAGCAGATGGAAGTGACAGTCTAGAGGGATTTGTGCTATGTCACTCC
ATTGCTGGGGGGACAGGTTCTGGTCTGGGCTCCTACCTCTTAGAGCGACTGAACGACAGGTACCCCAAGA
AATTGGTGCAGACATACTCAGTGTTTCCCAACCAGGACGAGATGAGTGATGTAGTGGTCCAGCCCTACAA
CTCCCTCCTCACACTAAAGAGGCTGACCCAGAATGCGGACTGCGTGGTGGTGCTGGACAACACAGCCCTG
AACCGGATCGCCACAGACCGCCTACACATCCAGAACCCATCCTTCTCCCAGATCAACCAGCTGGTGTCCA
CCATCATGTCAGCCAGCACCACCACCCTGCGCTACCCTGGATACATGAACAATGACCTCATCGGCCTCAT
CGCCTCGCTCATTCCCACCCCTCGGCTCCACTTCCTCATGACTGGCTACACCCCCCTCACCACGGACCAG
TCAGTGGCCAGTGTGAGGAAGACAACAGTCCTGGATGTCATGAGGCGCCTGCTACAGCCCAAGAATGTGA
TGGTGTCCACAGGTCGGGATCGTCAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGA
GGTAGACCCCACCCAGGTCCACAAGAGCCTGCAGAGGATCCGGGAAAGGAAACTGGCCAACTTCATTCCC
TGGGGCCCAGCCAGCATCCAGGTGGCCCTGTCAAGGAAGTCTCCCTACCTGCCCTCAGCCCACCGGGTCA
GCGGGCTCATGATGGCCAACCACACCAGTATCTCCTCGCTTTTTGAAAGTTCCTGCCAGCAGTATGACAA
ACTGTGGAAGCGGGGGGCCTTCCTGGAACAGTTTCGCAAGGAGGACATCTTCAAGGACAACTTTGAGGAG
ATGCACAGATCGAGGGAGGTGGTGCAGGAACTCATTGACGAGTACCACGCGGCCACCCGGCCGGACTACA
TCTCCTGGGGTACCCAGGAGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207203 protein sequence
Red=Cloning site Green=Tags(s)

MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLE
PRVIHSILNSSYAKLYNPENIYLSEHGGGAGNNWGRGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHS
IAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTAL
NRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQ
SVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIP
WGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFESSCQQYDKLWKRGAFLEQFRKEDIFKDNFEE
MHRSREVVQELIDEYHAATRPDYISWGTQEQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_134028
ORF Size 1353 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134028.2
RefSeq Size 1777 bp
RefSeq ORF 1356 bp
Locus ID 103768
UniProt ID Q8VCK3
Cytogenetics 11 D
MW 51.1 kDa
Summary Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_134028" in other vectors (4)
SKU Description Size Price
MC203136 Tubg2 (untagged) - Mouse tubulin, gamma 2 (Tubg2), (10ug) 10 ug
$457.00
MG207203 Tubg2 (tGFP-tagged) - Mouse tubulin, gamma 2 (Tubg2) 10 ug
$657.00
MR207203L3 Lenti ORF clone of Tubg2 (Myc-DDK-tagged) - Mouse tubulin, gamma 2 (Tubg2) 10 ug
$757.00
MR207203L4 Lenti ORF clone of Tubg2 (mGFP-tagged) - Mouse tubulin, gamma 2 (Tubg2) 10 ug
$757.00

Plasmid Map for MR207203

Download sequence for

Cloning scheme for MR207203

Circular map for MR207203

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