Tubb3 (NM_023279) Mouse Tagged ORF Clone
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Tubb3 |
| Synonyms | 3200002H15Rik; M(beta)3; M(beta)6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_023279 |
| ORF Size | 1350 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_023279.3 |
| RefSeq Size | 1758 bp |
| RefSeq ORF | 1353 bp |
| Locus ID | 22152 |
| UniProt ID | Q9ERD7 |
| Cytogenetics | 8 E1 |
| MW | 50.4 kDa |
| Summary | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_023279" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC201693 | Tubb3 (untagged) - Mouse tubulin, beta 3 (Tubb3), (10ug) | 10 ug |
$457.00
|
|
| MG207181 | Tubb3 (tGFP-tagged) - Mouse tubulin, beta 3 (Tubb3) | 10 ug |
$657.00
|
|
| MR207181L3 | Lenti ORF clone of Tubb3 (Myc-DDK-tagged) - Mouse tubulin, beta 3 (Tubb3) | 10 ug |
$757.00
|
|
| MR207181L4 | Lenti ORF clone of Tubb3 (mGFP-tagged) - Mouse tubulin, beta 3 (Tubb3) | 10 ug |
$757.00
|
|
Citations
iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications
Ou, Ball, Luan et al
Cell (2018)
Ou, Ball, Luan et al
Cell (2018)
Showing 1-1 of 1 papers.
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