Slc52a2 (NM_029643) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207176
Slc52a2 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 172B (Gpr172b)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc52a2
Synonyms 2010003P03Rik; D15Ertd747e; GPCR42; Gpr172b; mRFT1; PAR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207176 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCACCTCCGCTGGGCCGACTGGTGCTGACCCATCTGCTGGTGGCCCTTTTCGGCATGGGCTCCT
GGGCTGCTGTCAACGGGATCTGGGTAGAGCTGCCGGTAGTGGTGAAAGAGCTCCCGGAGGGTTGGAGCCT
CCCTTCCTACCTCTCTGTGCTTGTGGCGCTGGGAAACCTGGGTCTGCTGCTGGTGACTCTGTGGAGGCGT
CTGGCTCGCGGCAAGGGCGAGCAAGTCCCCATCCGAGTGGTTCAGGGGCTAGGCATAGTGGGCACAGGCC
TACTGGCCTCTCTGTGGAACCACGTGGCCCCAGTGGCAGGAAAGCCGTACTCAGTGGCCTTCCTAACGCT
GGCGTTTGTGCTGGCATTGGCCTGCTGTGCTTCTAATGTCACTTTCCTGCCCTTCTTGAGCCACCTCCCA
CCCCCCTTTTTACGGTCTTTCTTCCTGGGTCAGGGCCTGAGTGCCCTCCTGCCCTGTGTGCTAGCCCTGG
GTCAAGGGGTGGGCCGCCTTGAGTGCCTGCATGTGCCTGCCAACAGGACCACTGGGCCTCCGATCGAGGT
GTCTCCCATTAATTTCCCTGAACGGTTTTCTGCCACCACGTTCTTCTGGGTGTTGACTGCCCTTCTGGGC
ACTTCAGCAGCTGCCTTCCAAGGCCTCCTGTTACTACTGCCATCCCCGACATCTGAACCCACAACAGGTA
CAGGGCTTCGGGTAGAAACACCAGGAACAGAGGAGGAAGAGGAAGAGGAAGAGGCCTCACCTTTGCAGGA
GCCACCAGGCCAGGTGGCAGGCATTGTTTCCAGCCCAGACCCTAAGGCCCATCAGCTGTTCTCTTCCCGA
AGTGCCTGTCTACTGGGCCTGCTGGCCATCACCAATGCTCTGACCAATGGCGTGCTGCCCGCTGTACAGA
GCTTTTCCTGCCTGCCCTATGGGCGCCTGGCCTACCACTTGGCTGTCGTACTGGGTAGCTGTGCCAACCC
CCTTGCCTGCTTTCTGGCCATGGCAGTGCTGTGCAGGTCTCTGGCAGGGCTGTGTGGTCTGTCTCTGCTG
GGCATGCTCTTGGGCTCCTACCTGATGACACTGGCAGCCTTAAGTCCCTGTCCTCCTCTGGTGGGCACCT
CTGCAGGTGTGGTCCTCGTGGTACTCTCATGGGTCCTGTGCGCAGGCACGTTCTCGTATATCAAGGTGGC
GATCAGTTCCATGTTACACAGCGGAGGCCGGCCAGCACTGCTCGCTGCTGGCGTGGCTATTCAGGTGGGC
TCTCTGCTGGGTGCGGTCGCCATGTTCCCTCCTACCAGCATCTACCGAGTATTTCGCAGCGGAAAGGACT
GTGTGGACCAGTGTGGCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207176 protein sequence
Red=Cloning site Green=Tags(s)

MAAPPLGRLVLTHLLVALFGMGSWAAVNGIWVELPVVVKELPEGWSLPSYLSVLVALGNLGLLLVTLWRR
LARGKGEQVPIRVVQGLGIVGTGLLASLWNHVAPVAGKPYSVAFLTLAFVLALACCASNVTFLPFLSHLP
PPFLRSFFLGQGLSALLPCVLALGQGVGRLECLHVPANRTTGPPIEVSPINFPERFSATTFFWVLTALLG
TSAAAFQGLLLLLPSPTSEPTTGTGLRVETPGTEEEEEEEEASPLQEPPGQVAGIVSSPDPKAHQLFSSR
SACLLGLLAITNALTNGVLPAVQSFSCLPYGRLAYHLAVVLGSCANPLACFLAMAVLCRSLAGLCGLSLL
GMLLGSYLMTLAALSPCPPLVGTSAGVVLVVLSWVLCAGTFSYIKVAISSMLHSGGRPALLAAGVAIQVG
SLLGAVAMFPPTSIYRVFRSGKDCVDQCGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029643
ORF Size 1350 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029643.4
RefSeq Size 2700 bp
RefSeq ORF 1353 bp
Locus ID 52710
UniProt ID Q9D8F3
Cytogenetics 15 36.04 cM
MW 46.9 kDa
Summary Riboflavin transporter. Also acts as a receptor for 4-hydroxybutyrate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029643" in other vectors (4)
SKU Description Size Price
MC201310 Slc52a2 (untagged) - Mouse G protein-coupled receptor 172B (Gpr172b), (10ug) 10 ug
$686.00
MG207176 Slc52a2 (tGFP-tagged) - Mouse G protein-coupled receptor 172B (Gpr172b) 10 ug
$886.00
MR207176L3 Lenti ORF clone of Slc52a2 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 172B (Gpr172b) 10 ug
$986.00
MR207176L4 Lenti ORF clone of Slc52a2 (mGFP-tagged) - Mouse G protein-coupled receptor 172B (Gpr172b) 10 ug
$986.00

Plasmid Map for MR207176

Download sequence for

Cloning scheme for MR207176

Circular map for MR207176

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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