Gnal (NM_177137) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207159
Gnal (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha stimulating, olfactory type (Gnal), transcript variant 2
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gnal
Synonyms 2610011C15Rik; 9630020G10Rik; Galphaolf; Gna10; Golf
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207159 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCTATGCTACAGCCTGCGGCCGCTGCTCTTCGGGAGCCCAGAGGACACCCCGTGTGCGGCCTCGG
AACCCTGCGCAGAGGATGCTCAGCCCAGCGCCGCCCCGGCCCCTGCCTCGATCCCAGCCCCGGCTCCCGT
AGGGACCCTGCTCCGGCGTGGCGGCGGCCGGATCGTCGCGAACGCGCGGCCGCCAGGCGAGCTGCAGAGC
CGCCGGCGACAGGAGCAGCTACGAGCCGAGGAGCGCGAGGCGGCTAAAGAGGCGAGGAAAGTCAGCCGGG
GCATCGACCGCATGCTGCGCGAGCAGAAGCGGGACCTGCAGCAGACGCACCGGCTCCTGCTGCTGGGGGC
TGGTGAGTCCGGGAAAAGCACTATCGTCAAACAGATGAGGATCCTGCACGTCAATGGCTTCAACCCCGAG
GAAAAGAAGCAGAAAATTCTGGACATCAGGAAAAATGTCAAAGATGCGATCGTGACAATCGTTTCAGCAA
TGAGTACTATCATACCTCCAGTTCCACTGGCCAACCCTGAGAACCAGTTCCGGTCAGATTATATCAAGAG
CATAGCCCCTATCACTGACTTTGAATATTCCCAGGAGTTCTTTGACCATGTGAAGAAGCTGTGGGACGAT
GAAGGAGTGAAGGCCTGCTTTGAGAGATCCAACGAGTACCAGCTGATCGACTGTGCACAATACTTCCTGG
AAAGGATTGACAGTGTCAGTCTGGTTGACTACACACCCACAGACCAGGACCTGCTCAGATGCAGAGTGCT
GACATCAGGAATCTTTGAGACACGATTCCAAGTGGACAAAGTGAACTTTCACATGTTTGATGTTGGAGGC
CAGAGAGATGAGAGAAGAAAATGGATCCAGTGTTTTAATGATGTCACTGCGATCATTTACGTGGCGGCCT
GTAGTAGCTACAACATGGTGATCCGGGAAGATAACAATACCAACAGACTTCGGGAATCACTGGACCTGTT
TGAAAGCATCTGGAATAACAGGTGGTTGCGAACCATTTCTATCATCCTATTCTTGAACAAACAAGACATG
CTGGCAGAAAAAGTCTTGGCAGGGAAGTCAAAAATCGAAGACTATTTCCCGGAGTATGCCAATTATACTG
TCCCTGAAGATGCAACACCAGATGCGGGAGAAGATCCCAAAGTTACAAGAGCAAAGTTCTTTATCCGGGA
TCTGTTCTTGAGGATCAGCACAGCCACGGGTGATGGCAAACATTACTGCTACCCTCACTTCACCTGCGCC
GTGGACACAGAGAACATCCGCAGAGTGTTCAACGATTGCCGTGACATCATCCAGAGAATGCATCTCAAGC
AGTACGAACTCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207159 protein sequence
Red=Cloning site Green=Tags(s)

MGLCYSLRPLLFGSPEDTPCAASEPCAEDAQPSAAPAPASIPAPAPVGTLLRRGGGRIVANARPPGELQS
RRRQEQLRAEEREAAKEARKVSRGIDRMLREQKRDLQQTHRLLLLGAGESGKSTIVKQMRILHVNGFNPE
EKKQKILDIRKNVKDAIVTIVSAMSTIIPPVPLANPENQFRSDYIKSIAPITDFEYSQEFFDHVKKLWDD
EGVKACFERSNEYQLIDCAQYFLERIDSVSLVDYTPTDQDLLRCRVLTSGIFETRFQVDKVNFHMFDVGG
QRDERRKWIQCFNDVTAIIYVAACSSYNMVIREDNNTNRLRESLDLFESIWNNRWLRTISIILFLNKQDM
LAEKVLAGKSKIEDYFPEYANYTVPEDATPDAGEDPKVTRAKFFIRDLFLRISTATGDGKHYCYPHFTCA
VDTENIRRVFNDCRDIIQRMHLKQYELL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177137
ORF Size 1344 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177137.5, NP_796111.2
RefSeq Size 5654 bp
RefSeq ORF 1347 bp
Locus ID 14680
Cytogenetics 18 39.85 cM
MW 51.4 kDa
Summary Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177137" in other vectors (4)
SKU Description Size Price
MC215899 Gnal (untagged) - Mouse guanine nucleotide binding protein, alpha stimulating, olfactory type (Gnal), transcript variant 2, (10ug) 10 ug
$503.00
MG207159 Gnal (tGFP-tagged) - Mouse guanine nucleotide binding protein, alpha stimulating, olfactory type (Gnal), transcript variant 2 10 ug
$657.00
MR207159L3 Lenti ORF clone of Gnal (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha stimulating, olfactory type (Gnal), transcript variant 2 10 ug
$757.00
MR207159L4 Lenti ORF clone of Gnal (mGFP-tagged) - Mouse guanine nucleotide binding protein, alpha stimulating, olfactory type (Gnal), transcript variant 2 10 ug
$757.00

Plasmid Map for MR207159

Download sequence for

Cloning scheme for MR207159

Circular map for MR207159

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